In VitroAmoebicidal Activity ofSalvia stamineaandSalvia caespitosaonAcanthamoeba castellaniiand Their Cytotoxic Potentials on Corneal Cells

Göze, İsmihan; Alim, Ahmet; Dağ, Şeker; Tepe, Bektaş; Polat, Zübeyde Akın

doi:10.1089/jop.2008.0132

Cited by 23 publications

(13 citation statements)

References 39 publications

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“…Moreover, some antiamebic drugs show only an amebistatic effect, molecules used for keratitis treatment are toxic for the host cells, and some eye drop preparations are not well tolerated when used for prolonged periods (Fiori et al 2006). More recently, some natural agents from plant origin showing amebicidal effect on Acanthamoeba trophozoites have been described (Goze et al 2009;Ródio et al 2008;Polat et al 2007). …”

Section: Resultsmentioning

confidence: 99%

“…Although drug therapy for Acanthamoeba keratitis has been revolutionized with the introduction of combination chemotherapy (Martinez et al 2000;Kumar and Lloyd 2002;Mills 2003), failures can still occasionally occur. Thus, further research is needed to exploit new therapeutic agents and strategies (Schuster and Visvesvara 1998;Ródio et al 2008;Goze et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Bacteriocin-like substance from Bacillus amyloliquefaciens shows remarkable inhibition of Acanthamoeba polyphaga

et al. 2010

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The effectiveness of a bacteriocin-like substance (BLS) produced by Bacillus amyloliquefaciens was tested against Acanthamoeba polyphaga strains, and its cytotoxic potential on Vero cells was investigated. Amebicidal activity of the purified BLS was tested by plate bioassays with concentrations ranging from 12.5 to 6,400 AU mL(-1). Damage to A. pholyphaga cells was monitored using an inverted microscope and counted in a Fuchs-Rosenthal chamber after 24, 48, and 72 h. According to the results obtained, the BLS showed remarkable amebicidal and amebostatic effect on A. polyphaga and showed no cytotoxicity on the Vero cells. These results may have great relevance in the development of new acanthamoebicidal compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacteriocin-like substance from Bacillus amyloliquefaciens shows remarkable inhibition of Acanthamoeba polyphaga

et al. 2010

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“…With continued medical treatment, the iris cells die, the lens cells die, and cataract develops (Ehlers and Hjortdal 2004). Medicinal plants as Thymus (Polat et al 2007a), Salvia staminea (Goze et al 2009), Ipomoea sp., Kaempferia galanga, Cananga odorata (Chu et al 1998), Teucrium polium, Teucrium chamaedrys (Tepe et al 2011a, b), Pastinaca armenea, Inula oculus-christi , *p<0.05, statistically significant difference in comparison to non-treated control in the same time interval; **p<0.001, statistically highly significant difference in comparison to non-treated control in the same time interval; ***p<0.05, statistically significant difference in comparison to drug control in the same time interval *p<0.05, statistically significant difference in comparison to non-treated control in the same time interval; **p<0.001, statistically highly significant difference in comparison to non-treated control in the same time interval; ***p<0.05, statistically significant difference in comparison to drug control in the same time interval…”

Section: Discussionmentioning

confidence: 99%

“…Antiparasitic properties of many new natural product groups have been identified with their surprising efficacy and selectivity such as plant-derived alkaloids, terpenes, and phenolics (Kayser et al 2003). Several substances obtained from plants have been studied for the amoebicidal activity against Acanthamoeba, and many of these compounds have proven to be more effective than the currently used therapy (Polat et al 2007a(Polat et al , 2008Goze et al 2009). …”

Section: Introductionmentioning

confidence: 99%

In vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L., Curcuma longa L. and Pancratium maritimum L. on Acanthamoeba castellanii cysts

et al. 2011

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Acanthamoeba castellanii causes amoebic keratitis which is a painful sight-threatening disease of the eyes. Its eradication is difficult because the amoebas encyst making it highly resistant to anti-amoebic drugs, but several medicinal plants have proven to be more effective than the usual therapy. This study aimed to evaluate the in vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L. (peanut), Curcuma longa L. (turmeric), and Pancratium maritimum L. (sea daffodil) on A. castellanii cysts. Acanthamoeba were isolated from keratitic patients, cultivated on 1.5% non-nutrient agar, and then incubated with different concentrations of plant extracts which were further evaluated for their cysticidal activity. The results showed that all extracts had significant inhibitory effect on the multiplication of Acanthamoeba cysts as compared to the drug control (chlorhexidine) and non-treated control, and the inhibition was time and dose dependent. The ethanol extract of A. hypogaea had a remarkable cysticidal effect with minimal inhibitory concentration (MIC) of 100 mg/ml in all incubation periods, while the concentrations of 10 and 1 mg/ml were able to completely inhibit growth after 48 and 72 h, respectively. The concentrations 0.1 and 0.01 mg/ml failed to completely inhibit the cyst growth, but showed growth reduction by 64.4-82.6% in all incubation periods. C. longa had a MIC of 1 g and 100 mg/ml after 48 and 72 h, respectively, while the concentrations 10, 1, and 0.1 mg/ml caused growth reduction by 60-90.3% in all incubation periods. P. maritimum had a MIC of 200 mg/ml after 72 h, while the 20-, 2-, 0.2-, and 0.02-mg/ml concentrations showed growth reduction by 34-94.3% in all incubation periods. All extracts seemed to be more effective than chlorhexidine which caused only growth reduction by 55.3-80.2% in all incubation periods and failed to completely inhibit the cyst growth. In conclusion, ethanol extracts of A. hypogaea, C. longa, and P. maritimum could be considered a new natural agent against the Acanthamoeba cyst.

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“…The Lamiaceae plant family extracts were investigated for their parasitic properties: the methanolic extract of Salvia staminea and Salvia caespitosa (Goze et al 2009); the aqueous and hydro-alcoholic extract of Plectranthus punctatus (Tadesse et al 2009) and Plectranthus amboinicus (Periyanayagam et al 2008); the essential oils of Ocimum suave and Ocimum kilimandscharicum (Kweka et al 2008a, b); the crude leaves carbon-tetrachloride, methanol, and petroleum ether extracts of Ajuga remota (Sharma et al 2004); and hot-water extract Origanum onites (Erler et al 2009) were evaluated against Acanthamoeba castellanii, Haemonchus contortus, Plasmodium berghei yoelii, Anopheles arabiensis, Anopheles gambiae, Anopheles stephensi, Culex quinquefasciatus, and Megaselia halterata.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of botanical extracts against Haemaphysalis bispinosa Neumann and Hippobosca maculata Leach

Zahir¹,

Rahuman²,

Bagavan³

et al. 2010

Parasitol Res

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In the current study, in vitro evaluation of crude hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Anisomeles malabarica (L.) R. Br., Gloriosa superba L., Psidium guajava L., Ricinus communis L., and Solanum trilobatum L. exhibited acaricidal and insecticidal activities against the adult of Haemaphysalis bispinosa Neumann (Acarina: Ixodidae) and hematophagous fly Hippobosca maculata Leach (Diptera: Hippoboscidae). All plant extracts showed moderate toxic effect on parasites after 24 h of exposure; the complete inhibition (100%) at the maximum concentration tested (3,000 ppm) was obtained in acetone, methanol, hexane, and chloroform extracts of A. malabarica; methanol, chloroform, and ethyl acetate extracts of G. superba; acetone extract of P. guajava; methanol extract of R. communis; and leaf hexane extract of S. trilobatum; and the lowest inhibition (38%) was recorded for the seed hexane extract of S. trilobatum. The highest parasite dead was found in leaf acetone and methanol extracts of A. malabarica, seed methanol of G. superba, leaf methanol of R. communis against H. bispinosa (LC(50) = 466.15, 719.78, 476.06, and 243.87 ppm; LC(90) = 1,837.96, 2,014.47, 1,904.36, and 2,692.15 ppm), leaf hexane and chloroform extracts of A. malabarica, seed chloroform and ethyl acetate of G. superba, leaf acetone of P. guajava, leaf methanol of R. communis, and leaf hexane extract of S. trilobatum against H. maculata (LC(50) = 495.61, 414.81, 360.02, 479.37, 646.30, 506.13, and 432.77 ppm; LC(90) = 1,914.84, 1,956.59, 1,598.54, 1,636.41, 2,192.73, 1,982.66, and 1,872.33 ppm), respectively. These results suggest that the leaf methanol of R. communis, chloroform extracts of A. malabarica, and chloroform extract of G. superba have the potential to be used as an ideal eco-friendly approach for the control of the H. bispinosa and H. maculata. Therefore, this study provides first report on the parasitic activities of plant extracts from Southern India.

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In VitroAmoebicidal Activity ofSalvia stamineaandSalvia caespitosaonAcanthamoeba castellaniiand Their Cytotoxic Potentials on Corneal Cells

Abstract: Methanolic extract of S. staminea could be considered a new natural agent against Acanthamoeba. However, further evaluation by in vivo testing is needed to confirm the efficiency of its biological effect.

Cited by 23 publications

References 39 publications

Bacteriocin-like substance from Bacillus amyloliquefaciens shows remarkable inhibition of Acanthamoeba polyphaga

Bacteriocin-like substance from Bacillus amyloliquefaciens shows remarkable inhibition of Acanthamoeba polyphaga

In vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L., Curcuma longa L. and Pancratium maritimum L. on Acanthamoeba castellanii cysts

Evaluation of botanical extracts against Haemaphysalis bispinosa Neumann and Hippobosca maculata Leach

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