<i>In Vitro</i>
            Activities of Daptomycin-, Vancomycin-, and Teicoplanin-Loaded Polymethylmethacrylate against Methicillin-Susceptible, Methicillin-Resistant, and Vancomycin-Intermediate Strains of Staphylococcus aureus

Chang, Yu‐Han; Chen, Wen-Chien; Hsieh, Pang‐Hsin; Chen, Dave W.; Lee, Mel S.; Shih, Hsin-Nung; Ueng, Steve Wen–Neng

doi:10.1128/aac.05312-11

Cited by 67 publications

(50 citation statements)

References 22 publications

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“…The antibiotic elution characteristics of antibiotic-loaded bone cements have been widely investigated. However, the majority of these studies have been based on in vitro models (3,15). In vitro studies of specimens prepared under laboratory conditions do not necessarily address actual clinical circumstances.…”

Section: Discussionmentioning

confidence: 99%

“…Vancomycin, a glycopeptide antibiotic, remains the standard therapeutic agent used in most MRSA infections. Both oxacillin (16) and vancomycin (3) have been shown to be stable in PMMA and are released in a microbiologically active form. Therefore, the use of oxacillin-or vancomycin-loaded bone cement may be an effective method to prevent S. aureus infection in TKA.…”

mentioning

confidence: 99%

“…Staphylococcus aureus is the most common organism implicated in the infection of the skeletal system (3,8,11). Oxacillin, a penicillinase-resistant ␤-lactam similar to methicillin, is widely used clinically to treat penicillin-resistant S. aureus infection (2).…”

mentioning

confidence: 99%

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Antibacterial Activity of Joint Fluid in Cemented Total-Knee Arthroplasty: an In Vivo Comparative Study of Polymethylmethacrylate with and without Antibiotic Loading

Ueng

Hsieh

Shih

et al. 2012

Antimicrob Agents Chemother

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The objective of this study was to evaluate the antibacterial activities of joint fluids of patients undergoing total-knee arthroplasty (TKA). Thirty patients who were scheduled for primary cemented TKA were enrolled in the study. The patients were grouped on the basis of whether the cement was without antibiotic loading (control group) or loaded with oxacillin (oxacillin group) or vancomycin (vancomycin group). Cefazolin was administered to every patient as the perioperative prophylactic antibiotic. Samples of joint fluids were collected from the knee joints at 8, 16, 24, 32, 40, and 48 h after prosthesis implantation. We assessed the bioactivities of the joint fluids against methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA). The antibiotic contents of the joint fluid samples were further evaluated by using high-performance liquid chromatography. Against MSSA, all joint fluid samples exhibited at least 24 h of bacterial inhibition activity. The oxacillin (43.2 h ؎ 2 h) and vancomycin (40.8 h ؎ 1.8 h) groups exhibited significantly longer durations of antibacterial activities than the control group (28 h ؎ 1.3 h; P < 0.05). However, antibacterial activity against MRSA was observed only in the vancomycin group. In conclusion, cefazolin, which was administered as a prophylactic antibiotic in TKA, exhibited good ability for knee joint penetration and was sufficient to inhibit MSSA during its administration. The use of antibiotic-loaded cement can prolong the antibacterial activity of joint fluid in TKA. Further, vancomycin-loaded cement had antibacterial activity against MRSA superior to that of cement loaded with oxacillin or without antibiotic loading.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Antibacterial Activity of Joint Fluid in Cemented Total-Knee Arthroplasty: an In Vivo Comparative Study of Polymethylmethacrylate with and without Antibiotic Loading

Ueng

Hsieh

Shih

et al. 2012

Antimicrob Agents Chemother

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“…[10][11][12] S. aureus is the most common organism implicated in skeletal system infections. 4,20 Approximately 20% of S. aureus isolates collected in Europe are reported to be methicillinresistant S. aureus, and, in US hospitals, the prevalence ranges from 33% to 55%. 21 Vancomycin, a glycopeptide antibiotic, remains the standard therapeutic agent for the treatment of Gram-positive pathogens, including resistant and nonresistant strains.…”

mentioning

confidence: 99%

“…21 Vancomycin, a glycopeptide antibiotic, remains the standard therapeutic agent for the treatment of Gram-positive pathogens, including resistant and nonresistant strains. 20 Common Gram-negative bacteria involved in osteomyelitis include Pseudomonas aeruginosa, E. coli, and Klebsiella pneumoniae.…”

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confidence: 99%

Biodegradable drug-eluting nanofiber-enveloped implants for sustained release of high bactericidal concentrations of vancomycin and ceftazidime: in vitro and in vivo studies

Hsu¹,

Chen²,

Tai³

et al. 2014

IJN

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We developed biodegradable drug-eluting nanofiber-enveloped implants that provided sustained release of vancomycin and ceftazidime. To prepare the biodegradable nanofibrous membranes, poly(D,L)-lactide-co-glycolide and the antibiotics were first dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol. They were electrospun into biodegradable drug-eluting membranes, which were then enveloped on the surface of stainless plates. An elution method and a high-performance liquid chromatography assay were employed to characterize the in vivo and in vitro release rates of the antibiotics from the nanofiber-enveloped plates. The results showed that the biodegradable nanofiber-enveloped plates released high concentrations of vancomycin and ceftazidime (well above the minimum inhibitory concentration) for more than 3 and 8 weeks in vitro and in vivo, respectively. A bacterial inhibition test was carried out to determine the relative activity of the released antibiotics. The bioactivity ranged from 25% to 100%. In addition, the serum creatinine level remained within the normal range, suggesting that the high vancomycin concentration did not affect renal function. By adopting the electrospinning technique, we will be able to manufacture biodegradable drug-eluting implants for the long-term drug delivery of different antibiotics.

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Polymeric Prosthetic Systems for Site‐Specific Drug Administration: Physical and Chemical Properties

Parisi

Manzano

Flor

et al. 2015

Handbook of Polymers for Pharmaceutical Technologies

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In Vitro Activities of Daptomycin-, Vancomycin-, and Teicoplanin-Loaded Polymethylmethacrylate against Methicillin-Susceptible, Methicillin-Resistant, and Vancomycin-Intermediate Strains of Staphylococcus aureus

Cited by 67 publications

References 22 publications

Antibacterial Activity of Joint Fluid in Cemented Total-Knee Arthroplasty: an In Vivo Comparative Study of Polymethylmethacrylate with and without Antibiotic Loading

Antibacterial Activity of Joint Fluid in Cemented Total-Knee Arthroplasty: an In Vivo Comparative Study of Polymethylmethacrylate with and without Antibiotic Loading

Biodegradable drug-eluting nanofiber-enveloped implants for sustained release of high bactericidal concentrations of vancomycin and ceftazidime: in vitro and in vivo studies

Polymeric Prosthetic Systems for Site‐Specific Drug Administration: Physical and Chemical Properties

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