<i>In vitro</i>activities of antimicrobial cationic peptides; melittin and nisin, alone or in combination with antibiotics against Gram-positive bacteria

Döşler, Sibel; Gerçeker, A. Alev

doi:10.1179/1973947812y.0000000007

Cited by 57 publications

(47 citation statements)

References 22 publications

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“…The MICs of nisin against 13 S. aureus isolates and ATCC 29213 ranged from 16 to 32 mg/mL, and the MIC values of CA ranged from 0.25 to 0.5 mg/mL. Nisin has been shown to inhibit S. aureus, and its MIC range was determined to be 2e32 mg/mL (Dosler & Gerceker, 2012), which was consistent with the value calculated in the present study. For CA, the MIC values against S. aureus were 0.31 mg/ mL (Shen et al, 2015) and 1.56 ml/mL (~0.26 mg/mL) (Sanla-Ead et al, 2012), which were similar to that calculated in the present study.…”

Section: Determination Of Alone and Synergistic Effectssupporting

confidence: 56%

Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk

et al. 2017

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Section: Determination Of Alone and Synergistic Effectssupporting

confidence: 56%

Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk

et al. 2017

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“…Environmental cues from within an established biofilm can activate dispersal mechanisms, in which some bacteria revert to the planktonic state and are shed from the biofilm to cause acute infections, including sepsis, or form new biofilms at secondary sites leading to chronic infection. Staphylococci, particularly MRSA stains, are a major cause of nosocomial infections due to resistance to numerous current antibiotics21, which is exacerbated by biofilm formation22. Antibiotics or combinational therapies are commonly used for the treatment of Staphylococcal biofilms.…”

Section: Discussionmentioning

confidence: 99%

Histones from Avian Erythrocytes Exhibit Antibiofilm activity against methicillin-sensitive and methicillin-resistant Staphylococcus aureus

Rose-Martel

Kulshreshtha

Berhane

et al. 2017

Sci Rep

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Staphylococcus aureus, a human pathogen associated with many illnesses and post-surgical infections, can resist treatment due to the emergence of antibiotic-resistant strains and through biofilm formation. The current treatments for chronic biofilm infections are antibiotics and/or surgical removal of the contaminated medical device. Due to higher morbidity and mortality rates associated with overuse/misuse of antibiotics, alternate treatments are essential. This study reports the antibiofilm activity of avian erythrocyte histones against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Fluorescence and scanning electron microscopy revealed membrane damage to bacteria in histone-treated biofilms. Histones and indolicidin (positive control) increased the expression of apsS and apsR, which are associated with the Antimicrobial Peptide (AMP) sensor/regulator system in S. aureus. The expression of dltB, and vraF, associated with AMP resistance mechanisms, were under histone inducible control in the biofilm-embedded bacterial cells. The time kill kinetics for histones against S. aureus revealed a rapid biocidal activity (<5 min). Purified erythrocyte-specific histone H5 possessed 3–4 fold enhanced antimicrobial activity against planktonic cells compared to the histone mixture (H1, H2A, H2B, H3, H4, H5). These results demonstrate the promise of histones and histone-like derivatives as novel antibiotics against pathogens in their planktonic and biofilm forms.

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“…From an evolutionary ecological perspective, however, the interaction between different AMPs produced by the host itself is of prime interest. A number of studies, including transcriptomic data on AMPs, show that native AMPs are typically expressed in combination [44][45][46][47][48][49]. Often these expression patterns are assumed to support synergism between co-expressed AMPs-even though experimental evidence by knockouts is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Perspectives on the evolutionary ecology of arthropod antimicrobial peptides

Rolff

Schmid‐Hempel

2016

Phil. Trans. R. Soc. B

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One contribution of 13 to a theme issue 'Evolutionary ecology of arthropod antimicrobial peptides'. Antimicrobial peptides (AMPs) are important elements of the innate immune defence in multicellular organisms that target and kill microbes. Here, we reflect on the various points that are raised by the authors of the 11 contributions to a special issue of Philosophical Transactions on the 'evolutionary ecology of arthropod antimicrobial peptides'. We see five interesting topics emerging. (i) AMP genes in insects, and perhaps in arthropods more generally, evolve much slower than most other immune genes. One explanation refers to the constraints set by AMPs being part of a finely tuned defence system. A new view argues that AMPs are under strong stabilizing selection. Regardless, this striking observation still invites many more questions than have been answered so far. (ii) AMPs almost always are expressed in combinations and sometimes show expression patterns that are dependent on the infectious agent. While it is often assumed that this can be explained by synergistic interactions, such interactions have rarely been demonstrated and need to be studied further. Moreover, how to define synergy in the first place remains difficult and needs to be addressed. (iii) AMPs play a very important role in mediating the interaction between a host and its mutualistic or commensal microbes. This has only been studied in a very small number of (insect) species. It has become clear that the very same AMPs play different roles in different situations and hence are under concurrent selection. (iv) Different environments shape the physiology of organisms; especially the host-associated microbial communities should impact on the evolution host AMPs. Studies in social insects and some organisms from extreme environments seem to support this notion, but, overall, the evidence for adaptation of AMPs to a given environment is scant. (v) AMPs are considered or already developed as new drugs in medicine. However, bacteria can evolve resistance to AMPs. Therefore, in the light of our limited understanding of AMP evolution in the natural context, and also the very limited understanding of the evolution of resistance against AMPs in bacteria in particular, caution is recommended. What is clear though is that study of the ecology and evolution of AMPs in natural systems could inform many of these outstanding questions, including those related to medical applications and pathogen control.This article is part of the themed issue 'Evolutionary ecology of arthropod antimicrobial peptides'.

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In vitroactivities of antimicrobial cationic peptides; melittin and nisin, alone or in combination with antibiotics against Gram-positive bacteria

Cited by 57 publications

References 22 publications

Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk

Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk

Histones from Avian Erythrocytes Exhibit Antibiofilm activity against methicillin-sensitive and methicillin-resistant Staphylococcus aureus

Perspectives on the evolutionary ecology of arthropod antimicrobial peptides

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