Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk

Shi, Ce; Zhang, Xiaowei; Zhao, Xingchen; Meng, Rizeng; Liu, Zonghui; Chen, Xiangrong; Guo, Na

doi:10.1016/j.foodcont.2016.06.020

Cited by 89 publications

(64 citation statements)

References 49 publications

(44 reference statements)

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“…Synergistic effects are a positive interaction occurring when two antimicrobials are combined to exert an inhibiting effect, resulting in a higher activity than the sum of the individual effects (Kumar et al, 2014). Nis is known to act synergistically with several antimicrobials, for instance, chelators, small substances from plants, reuterin, proteins such as lactoferrin, milk-derived peptides, lysozyme (Shi et al, 2017) and lipids with anionic characteristics, such as rhamnolipids (Magalhaes and Nitschke, 2013). However, little information is available with respect to the antibacterial activity and mechanisms of Nis in combination with Cin or Car against gram-negative or even gram-positive bacteria.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Effects of Nisin Combined with Plant-derived Antimicrobials on Pathogenic Bacteria and the Interaction with Complex Food Systems

Klangpetch

Noma

2018

FSTR

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This study aimed to evaluate the synergistic effects of nisin combined with cinnamaldehyde (Nis+Cin) and nisin combined with carvacrol (Nis+Car) on food-borne bacteria. The minimum inhibitory concentrations for Nis, Cin and Car ranged from 2,500 to 10,000 IU/mL, 0.78-6.25 mg/mL and 0.78-3.13 mg/mL, respectively. Nis+Cin displayed a total synergism against all test bacteria, showing a marked release of bacterial intracellular constituents. In a food model, high protein concentration, low starch and oil concentration as well as low pH, positively influenced the antimicrobial activity. Moreover, in sandwich spread, Nis+Cin enhanced the inhibition of S. typhimurium at the greatest level by reducing cell numbers by more than 4 log CFU/g from 6 days of storage. These results suggest that the dose used for each antimicrobial compound can be minimized by combination, thereby decreasing the possibility of antimicrobial resistance and also reducing food processing costs.

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Section: Resultsmentioning

confidence: 99%

Inhibitory Effects of Nisin Combined with Plant-derived Antimicrobials on Pathogenic Bacteria and the Interaction with Complex Food Systems

Klangpetch

Noma

2018

FSTR

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“…According to preliminary checkerboard assays, the positive interaction between EDTA and the N8-r-lecCI supernatant against L. monocytogenes ATCC 19115 was chosen for time–kill assay in pasteurized milk. The growth curve-based time–kill assay was determined according to a previous method with minor modification ( Garcia et al, 2010 ; Shi et al, 2017 ). The experimental tubes containing pasteurized milk initially inoculated with 10 5 CFU/mL pathogens were added with either single antibacterial agent or the combination of antibacterial agents.…”

Section: Methodsmentioning

confidence: 99%

Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk

Qiao

et al. 2018

Front. Microbiol.

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Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the combined use of them could be a potential strategy for effective inhibition of foodborne pathogens. In this study, L. lactis N8-r-lecCI (N8 harboring lecCI gene) coexpressing nisin–leucocin C was constructed based on the food-grade carrier L. lactis N8. Production of both bacteriocins was stably maintained. Antimicrobial measurements showed that the recombinant strain is effectively against Listeria monocytogenes and Staphylococcus aureus and moderately against Salmonella enterica serovar Enteritidis and Escherichia coli because of its stronger antibacterial activity than the parental strain, this result first demonstrated that the co-expression of nisin and leucocin C results in highly efficient antimicrobial activity. The checkerboard assay showed that the antibacterial activity of L. lactis N8-r-lecCI supernatant was enhanced in the presence of low concentration of EDTA. Analysis of the scanning electron microscope image showed the biggest cellular morphology change in L. monocytogenes treated with a mixture of EDTA and L. lactis N8-r-lecCI supernatant. The practical effect was verified in pasteurized milk through time-kill assay. The L. lactis N8-r-lecCI strain expressing both nisin and leucocin C has a promising application prospect in pasteurized milk processing and preservation because of its strong antibacterial activity.

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“…19 The synergistic antibacterial activity of cinnamaldehyde and nisin has been reported to reduce the problem of Staphylococcus aureus resistance. 20 Although cinnamaldehyde and citral have been used against P. expansum, most previous works about their direct antifungal activities focused on the control or application efficacy in food packaging. 6,[21][22][23][24] The knowledge about their action modes on P. expansum is still incomplete.…”

Section: Introductionmentioning

confidence: 99%

Antifungal mechanism of cinnamaldehyde and citral combination against Penicillium expansum based on FT-IR fingerprint, plasma membrane, oxidative stress and volatile profile

et al. 2018

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Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk

Cited by 89 publications

References 49 publications

Inhibitory Effects of Nisin Combined with Plant-derived Antimicrobials on Pathogenic Bacteria and the Interaction with Complex Food Systems

Inhibitory Effects of Nisin Combined with Plant-derived Antimicrobials on Pathogenic Bacteria and the Interaction with Complex Food Systems

Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk

Antifungal mechanism of cinnamaldehyde and citral combination against Penicillium expansum based on FT-IR fingerprint, plasma membrane, oxidative stress and volatile profile

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