In vitroacetylcholinesterase activity of peptide derivatives isolated from two species of Leguminosae

Abstract

Context: Cratylia mollis Martius ex Benth. and Cenostigma macrophyllum Tul. (Leguminosae) are both endemic Brazilian plants and they are used by the natives as medicinal plants, and the leaves of C. mollis are also employed as forage for cattle during the dry season of region. Objective: Isolation of the compounds responsible for the acetylcholinesterase (AChE) inhibition from the CHCl 3 active extract. Materials and methods: Two peptidic compounds were isolated by chromatographic techniques from the CHCl 3 extract of the leaves of C. mollis and C. macrophyllum. They were identified by spectrometric data analysis (MS and NMR) and they were subjected to AChE inhibition employing Ellman's test.

Evaluation of anti-inflammatory, antinociceptive and biological activities of Cenostigma macrophyllum standardized extracts and determination and quantification of the main metabolites

“…This result shows excellent activity of this extract. Previously, from leaves of C. macrophyllum were isolated two peptides inhibitors of AChE 12 but the compounds present in the extracts did not show AChE inhibition.…”

“…The in vitro inhibition of AChE was determined by the spectrophotometric method based on the methodology previously described. 12 Solutions of 10 mg mL −1 of extracts and positive control were prepared in HPLC grade ethanol. To perform the test, 140 μL of phosphate buffer (0.1 M) pH 7.5 with 0.1% bovine serum albumin, 20 μL of the extracts, standard (physostigmine, Sigma ≥99%, was used as positive control) or ethanol (blank) and 20 μL of AChE 5.0 U mL −1 , were added on a microplate reader, all the experiments were carried out in triplicate.…”

“…[8][9][10] Phytochemical studies with C. macrophyllum have led to the isolation and identication of avonoids, biavonoids, benzoic acid derivatives, peptide derivatives, triterpenoids, steroids, vitamin A and E, fatty acids, coumarin and volatile esters. 3,11,12 However, there are no reports in the literature on the development of a validated chromatographic method for the quantication of active principles present in this species. Thus, in the present study, a HPLC-DAD method was developed to simultaneously detect and quantify ve bioactive compounds ( Fig.…”

Melipona scutellaris Geopropolis: Chemical Composition and Bioactivity

“…Free radical scavenging is one of the methodologies frequently used to assess antioxidant capacity [20]. DPPH free radical scavenging measures the ability of the analyzed substances to donate hydrogen ions [49]. The reducing power of a given product indicates its antioxidant potential [20].…”