In situ reactive extraction of lactic acid from fermentation media

Abstract

Extractive lactic acid fermentation was investigated in the presence of sun¯ower oil and Alamine-336 (with oleyl alcohol as the diluent solvent). Lactic acid was produced in various media at 37°C using Lactobacillus delbrueckii (NRRL-B 445). First, the effects of oleyl alcohol (33.3%, v/v), immobilisation, and immobilisation in the presence of sun¯ower oil (5, 10, 15%, v/v) on lactic acid production were investigated. It was found that oleyl alcohol did not affect production while addition of sun¯ower oil increased lactic acid production from 10.22 to 16.46 gdm À3 . On the other hand, a toxic effect was observed for oleyl alcohol solutions containing 15±50% (v/v) Alamine-336. A maximum total lactic acid concentration of 25.59 gdm À3 was obtained when an oleyl alcohol solution containing 15%

Mass Transfer Investigation of Organic Acid Exatraction with Trioctylamine and Aliquat 336 Dissolved in Various Solvents

“…a pH of 4.5 for the synthetic fermentation broth (compared to pH 2.4 for pure lactic acid) and presence of salts did not significantly affect the extraction efficiency. These results compare well with those in the literature (Tik, et al, 2001;Yankov, et al, 2005).…”

“…The extractive fermentation, in situ application of the solvent extraction technique, keeps the product concentration in the broth at a low level and suppresses the product inhibition by continuously removing them from a fermentation broth [Siebold, et al, 1995;Yankov et al, 2005;Frieling and Schugerl, 1999]. Various methods for the extraction of lactic acid have been reported such as precipitation, ion exchange process, adsorption, diffusion dialysis, microcapsules, esterification and hydrolysis, reactive extraction as well as a simulated moving bed process (Hong, et al, 2002;Tik, et al, 2001;Tong, et al, 1999;Ju, and Verma, 1994;Gong, et al, 2006;Sun et al, 2006). These methods have several disadvantages including high cost, and they produce large volumes of waste, require multiple steps, and operate with low efficiency under practical conditions.…”

Ca-Alginate Liquid Core Capsule for Lactobacili Fermentation

“…Besides that, the liquid core capsules could be used to counteract the effect caused by the harsh conditions. The issue of low pH and present of inhibitor like lactate could be addressed by introducing encapsulated neutralizing agents in liquid core capsule in the fermentation media (Lee et al 2013;Tik et al 2001). The tested neutralizing agents are Alamine-336 in oleyl alcohol and calcium carbonate (Lee et al 2013;Tik et al 2001).…”

“…The issue of low pH and present of inhibitor like lactate could be addressed by introducing encapsulated neutralizing agents in liquid core capsule in the fermentation media (Lee et al 2013;Tik et al 2001). The tested neutralizing agents are Alamine-336 in oleyl alcohol and calcium carbonate (Lee et al 2013;Tik et al 2001). Since the capsules have low mass transfer resistance, the solutes/ions could be freely diffused between the inner liquid core and the fermentation media through the capsular membrane to react with the inhibitors (Lee et al 2013;Whelehan et al 2010;Marison et al 2004;Koyama and Seki 2004b;Chai et al 2004;Wyss et al 2004;Stark et al 2003).…”

Extraction‐Fermentation Hybrid (Extractive Fermentation)

“…Reactive extraction with long-chain aliphatic amines such as tricaprylyl amine (Alamine 336) and ditridecyl amine (Adogen 283), which have low solubility in water and high distribution coefficients for carboxylic acids, has therefore been more commonly used to recover carboxylic acids from dilute solutions (Yang et al ., 1991;King 1992;Eyal and Canari, 1995;Tik et al ., 2001). Depending on the carboxylic acid and amine extractant, the distribution coefficient usually ranges from ∼3 to over 20.…”