In situmicroextraction method to determine the viscosity of biofluid in threadlike structures on the surfaces of mammalian organs

Abstract

We report a method to measure the viscosity of microL volumes of biofluid obtained from threadlike structures (NTSs) on the surfaces of mammalian (rabbit) internal organs. The fluid was mechanically microextracted in situ from NTSs on the organ surfaces by a glass capillary connected to an extractor. From the Brownian motion of the 0.8+/-0.1microm diameter granules in the extracted fluid, the fluid viscosity was determined to be 1.4+/-0.1mPa s at room temperature. This viscosity is comparable to the viscosity of rabbit blood plasma.

Characterization of the Primo-Vascular System in the Abdominal Cavity of Lung Cancer Mouse Model and Its Differences from the Lymphatic System

“…Furthermore, some evidence for a fluid-conducting function of the primo-vascular system has been provided by measurements of the flow speed [7] and the fluid viscosity [8].…”

A cytological observation of the fluid in the primo-nodes and vessels on the surfaces of mammalian internal organs

“…Very recently, some morphological features of primomicrocells have been studied (Ogay et al 2006;Kwon et al 2007;Baik et al 2009), providing evidence for the circulatory function of the primo-vessels (Shin et al 2005;Lee et al 2007;Sung et al 2008Sung et al , 2009.…”

Evidence for an Additional Metastatic Route: In Vivo Imaging of Cancer Cells in the Primo-Vascular System Around Tumors and Organs

“…Although the entire primo-vascular circulatory network has not yet been elucidated, evidence for a fluid-conducting function of the primo-vascular system was observed via an electron microscopic examination of the threadlike structures which contain both fluid and very small cells of 1∼2 μm in diameter [9]. Measurement of flow speed using microinjection of dye (0.3±0.1 mm/s) [10] and measurement of fluid viscosity using a microextraction method (1.4±0.1 mPa s at RT) have also been reported [11].…”