A cytological observation of the fluid in the primo-nodes and vessels on the surfaces of mammalian internal organs

Abstract

Abstract:We report on the preliminary cytological observation of fluid in the primo-nodes and vessels on the surfaces of the internal organs of mammals. With some microsurgical procedures, we observed many cells and microcells that spread out of the nodes on the organ surfaces of rats and rabbits. These cells generally showed the following morphologies: (1) round or oval cells, 10 µm in size, with predominantly little cytoplasm; (2) cells with nuclei that exhibited a collapsed shape; (3) binucleated cells, 20 µm in size; (4) spherical granules, ranging 0.5-2.0 µm in size (primo-microcells); and (5) aggregations of such granules. These findings on the existence of cells with diverse morphologies in the fluid of primo-nodes and vessels could be evidence supporting the hypothesis that the anatomical basis of acupuncture meridians (i.e., primo-vascular system) may be a migration channel for various kind of cells.

Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining

“…Our overall findings were similar to those of recent studies using H&E staining and electron microscopy that showed the presence of numerous immune cells in the PVS, such as MCs, macrophages, and neutrophils [14–17]. We attempted to observe the PVS cells in tissue as well as the single cells in isolation from PVS tissue for more decisive observation of PVS cells.…”

“…In this study, the immune cells, such as MCs, neutrophils, eosinophils, and lymphocytes, accounted for the vast majority of the whole PVS cell population (~94%). The results strongly support that PVS may have a crucial role in the initiation and/or maintenance of immunological functions [14–17]. We found the degranulation of putative MCs in the sinuses of PVs, indicating that the MCs were preferentially activated in the sinuses in PVs rather than in PNs (Figure 2(b4)).…”

“…At the cellular level, the PVS contains various types of immune cells, such as macrophages, mast cells (MCs), and eosinophils, which implies the system's potential role in immune responses [14–17]. In addition, PVS cells have been categorized into four major types based on current-voltage (I-V) relations recorded from the cells in PN slices [7].…”

Network of Endocardial Vessels

“…Essential characteristic features of the primordial vascular system (endocardial circulatory system) described by Kim [1] were the thickness of the primordial vessels (endocardial vessels), the presence of the rod-shaped nuclei in a longitudinally aligned form, and the many cells in the primordial nodes (expansions), all of which are consistent with our data, as shown in figure 2a–e. The cell types inside the primordial nodes (expansions) have recently been studied morphologically [22]. In particular, the bundle-like structure observed in the distribution of the rod-shaped nuclei in figure 2e is also consistent with the claims of Kim [1].…”

Современные Взгляды На субстрат И механизмы Действия Акупунктуры

“…За [6,15]. В експериментах на щурах, в умовах штучно викликаної ішемії мозку шляхом перев'язки правої загальної сонної артерії та системної гіпоксії (8% O 2 протягом 20 хв) після ін'єкції в хвостову вену стовбурових клітин, виділених з первинних судин, розмір ішемізованої тканини істотно зменшувався.…”