In situ Hybridization for the Demonstration of Bovine Leukemia Virus Transcripts in Lymphosarcoma Cells Using Biotinylated Probes

Abstract

Bovine leukemia virus (BLV) RNA was demonstrated in peripheral blood lymphocytes treated with concanavalin A or phytohemagglutinin from a BLV-infected ox, and in lymphosarcoma cellscultured without mitogen from an enzootic bovine leukosis cow by in situ hybridization using biotinylated D N A probes. U.S.