Expression and quantification of IgG and IgM molecules on the surface of lymphocytes of cattle infected with bovine leukaemia virus

Levkut, M.; Ponti, W.; Soligo, Davide; Quirici, N.; Rocchi, Mara; Deliliers, G Lambertenghi

doi:10.1016/0034-5288(95)90029-2

Cited by 6 publications

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“…The reduced expression of L-selectin was also observed on large cells, supposed as blasts, that possessed a high level of sIgM. Cells with lymphoblastoid shape, larger than normal B cells, and with the high IgM expression were observed in BLV + PL + animals by electron scanning microscopy (Levkut et al, 1995). The expression of L-selectin in EBL has not been reported, but several studies indicate changes of expression of other cell surface molecules (MHC class I and II, CD4, CD8, CD11, CD14, CD25, CD32) (Taylor et al, 1992;Stone et al, 1995Stone et al, , 1997Werling et al, 1998).…”

Section: Discussionmentioning

confidence: 86%

“…The reduced expression of L‐selectin was also observed on large cells, supposed as blasts, that possessed a high level of sIgM. Cells with lymphoblastoid shape, larger than normal B cells, and with the high IgM expression were observed in BLV + PL + animals by electron scanning microscopy (Levkut et al . 1995).…”

Section: Discussionmentioning

confidence: 97%

“…Cows which developed PL underwent proliferation of B lymphocytes which expressed immunoglobulins and CD5 antigens (Depelchin et al, 1989;Meirom et al, 1993). Changes in the expression of cell surface molecules in BLV-infected cows have been reported (Taylor et al, 1992;Stone et al, 1995Stone et al, , 1997Levkut et al, 1995;Werling et al, 1998).…”

Section: Introductionmentioning

confidence: 97%

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Decreased expression of L‐selectin (CD62L) on lymphocytes in enzootic bovine leukaemia

Dušinský

Bardotti

Ponti

2000

Journal of Veterinary Medicine, Series B

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Expression of L-selectin was determined by single- and two-colour immunofluorescence on granulocytes, peripheral blood mononuclear cells (PBMC) and blasts of bovine origin by means of a monoclonal antibody IVA94 which recognizes bovine L-selectin (CD62L). Cells were separated from peripheral blood of healthy cattle and colleagues infected with bovine leukaemia virus (BLV). BLV-infected animals comprised lymphocytotic and non-lymphocytotic cows. L-selectin was expressed on 90-98% of granulocytes in all tested animals. The percentage of PBMC expressing L-selectin was lower in cattle with persistent lymphocytosis than in non-lymphocytotic or BLV-free cattle, and inversely correlated with lymphocyte counts. The ratio of B lymphocytes stained for L-selectin was significantly decreased from 60.2 +/- 1.9% in BLV-free cattle to 43.8 +/- 3.6 and 22.5 +/- 5.7% in non-lymphocytotic and lymphocytotic cattle, respectively. B-lymphocytes stained for L-selectin exhibited about 50% reduction in L-selectin expression in BLV-infected cattle compared with BLV-free cattle, as judged by the mean fluorescence intensity (MFI). The percentage of L-selectin-positive PBMC not bearing surface immunoglobulin M (predominantly T lymphocytes) was comparable in BLV-free and BLV-infected cattle. However, L-selectin expression on T lymphocytes was reduced (about 50%) in BLV-infected cattle, as judged by the MFI. We suppose that BLV infection results in a decreased L-selectin expression on lymphocytes, and accordingly, it may contribute to deregulation of the host immune system.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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Decreased expression of L‐selectin (CD62L) on lymphocytes in enzootic bovine leukaemia

Dušinský

Bardotti

Ponti

2000

Journal of Veterinary Medicine, Series B

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“…O VLB infecta, principalmente, linfócitos B , expressando, em sua superfície, o complexo principal de histocompatibilidade de classe II (MHC-II + ), imunoglobulina M de superfície (sIgM + ) (LEVY et al, 1987;MIRSKY et al, 1996), assim como os marcadores celulares (ou grupamentos de diferenciação -CD) CD5 e CD11b, com algumas flutuações para os últimos três marcadores nos estágios terminais da enfermidade (GILLET et al, 2007). Por sua vez, o HTLV-1 claramente infecta outros tipos celulares:…”

Section: Introductionunclassified

“…Posto que a utilização de vacinas para o controle da LEB ainda não apresenta resultados consistentes SUHRBIER, 2001;USUI et al, 2003;ALTANEROVA et al, 2004), entende-se que a detecção e a identificação dos bovinos sororreagentes positivos aos antígenos do VLB e sua retirada dos rebanhos ainda seja o princípio fundamental para se evitar a disseminação da enfermidade (MOLLOY et al, 1994). LEWIN, 1989;XU et al, 1993;ZANOTTI et al, 1996) e ovino (OLA) (KONNAI et al, 2003a;DUKKIPATI et al, 2006) LEVKUT et al, 1995).…”

Section: Introductionunclassified

Influência do vírus da lecucose bovina na resposta imunitária de animais naturalmente infectados

Azedo¹

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No início de 2003, resolvi retornar a São Paulo para ter a oportunidade de ficar mais próximo de meus pais, à época, já com idade. A experiência dos 13 anos na Amazônia como, principalmente, buiatra, não seria inerente na busca por uma recolocação nesta cidade... e eu estava ciente disto. Mas, em mim, já havia surgido a vocação para a docência... uma possibilidade. No entanto, seria necessária a busca por aperfeiçoamento, aprender a ensinar... a responder e a perguntar. Seriam prementes Mestrado e Doutorado. Finda mais esta etapa, certo é que, a cada dia, ainda há muito que aprender, mas a vocação que surgira tornou-se paixão... Muitos foram fundamentais para que estes últimos anos fossem agradáveis e produtivos. Em especial, agradeço: À minha querida Amiga, Irmã e O Or ri ie en nt ta ad do or ra a, , P Pr ro of f a a D Dr r a a A Al li ic ce e M Ma ar ri ia a M Me el lv vi il ll le e P Pa ai iv va a D De el ll la a L Li ib be er ra a, , para sempre "m mi in nh ha a c ch he ef fi in nh ha a". Essencial... especial. Pelas conversas e conselhos, pelos ensinamentos, pela paciência, pela confiança, pelos exemplos de profissional prestativa e dedicada, de mãe, filha e esposa, sou eterna e incondicionalmente grato. À valiosa equipe formada pelos Médicos Veterinários Pós-Graduandos M Ma ai ia ar ra a G Ga ar rc ci ia a B Bl la ag gi it tz z, , C Ca am mi il la a F Fr re ei it ta as s B Ba at ti is st ta a, , T Ta at ti ia an na a d de e R Re ez ze en nd de e S Sp pí ín no ol la a, , M Me el li is ss sa a H Ha ar rt tm ma an n, , C Cl la au ud di ia a P Pe es st ta an na a R Ri ib be ei ir ro o, , B Bá ár rb ba ar ra a G Ga ab br ri ie el la a S So oa ar re es s S Sa an nc ch he es s e e F Fe er rn na an nd do o N No og gu ue ei ir ra a d de e S So ou uz za a. Pessoas tão heterogêneas e tão intensas e dedicadas. Nossas pesquisas, nossos trabalhos são fruto do esforço de cada um de nós. Nosso fim... o desenvolvimento de todos. Obrigado por tudo! Aos diletos Professores Doutores do Departamento de Clínica Médica, A Ar rc ch hi iv va al ld do o R Re ec ch he e J Jú ún ni io or r, , C Ca ar rl la a B Ba ar rg gi i B Be el ll li i, , C Ca ar rl lo os s E Ed du ua ar rd do o L La ar rs ss so on n, , C Cá ás ss si io o X Xa av vi ie er r d de e M Me en nd do on nç ça a J Jú ún ni io or r, , D De en ni is se e S Sa ar re et tt ta a S Sc ch hw wa ar rt tz z, , E Ed du ua ar rd do o H Ha ar rr ry y B Bi ir rg ge el l J Jú ún ni io or r, , E En nr ri ic co o L Li ip pp pi i O Or rt to ol la an ni i, , F Fe er rn na an nd do o J Jo os sé é B Be en ne es si i, , L Li il li ia am m G Gr re eg go or ry y, , M Má ár rc ci ia a M Me er ry y K Ko og gi ik ka a, , M Ma ar ri ia a C Cl lá áu ud di ia a A Ar ra ar ri ip pe e S Su uc cu up pi ir ra a, , M Ma ar ri ia a H He el le en na a A Ak ka ao o L La ar rs ss so on n, , M Mi it ti ik ka a K Ku ur ri ib ba ay ya as sh hi i H Ha ag gi iw wa ar ra a, , R Ra aq qu ue el l Y Yv vo on nn ne e A Ar ra an nt te es s B Ba ac cc ca ar ri in n, , S Si il lv vi ia a R Re eg gi in na a R Ri ic cc ci ...

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Immunoanalysis of IgG and IgM molecules on the surface of BLV infected cattle lymphocytes

Levkut

Ponti

Levkutová

et al. 1997

Comparative Haematology International

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Expression and quantification of IgG and IgM molecules on the surface of lymphocytes of cattle infected with bovine leukaemia virus

Cited by 6 publications

References 13 publications

Decreased expression of L‐selectin (CD62L) on lymphocytes in enzootic bovine leukaemia

Decreased expression of L‐selectin (CD62L) on lymphocytes in enzootic bovine leukaemia

Influência do vírus da lecucose bovina na resposta imunitária de animais naturalmente infectados

Immunoanalysis of IgG and IgM molecules on the surface of BLV infected cattle lymphocytes

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