In situgold nanoparticle growth on polydopamine-coated 3D-printed scaffolds improves osteogenic differentiation for bone tissue engineering applications:in vitroandin vivostudies

Lee, Sang Jin; Hyo-Jung, Lee; Kim, Sung-Yeol; Seok, Ji Min; Lee, Jun Hee; Kim, Wan Doo; Kwon, Il Keun; Park, Shin‐Young; Park, Su A

doi:10.1039/c8nr04037k

Cited by 78 publications

(44 citation statements)

References 58 publications

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“…Thus, it has been used in many studies. [42][43][44][45][46][47] Biocompatibility was assessed by incubating cells on the surface and inside of Gel-Ty hydrogel. Cell viability was not significantly different between hydrogels at all concentrations.…”

Section: Discussionmentioning

confidence: 99%

Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

Lee

Heo

Nah

et al. 2018

IJN

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BackgroundFor effective bone regeneration, it is necessary to implant a biocompatible scaffold that is capable of inducing cell growth and continuous osteogenic stimulation at the defected site. Here, we suggest an injectable hydrogel system using enzymatic cross-linkable gelatin (Gel) and functionalized gold nanoparticles (GNPs).MethodsIn this work, tyramine (Ty) was synthesized on the gelatin backbone (Gel-Ty) to enable a phenol crosslinking reaction with horseradish peroxidase (HRP). N-acetyl cysteine (NAC) was attached to the GNPs surface (G-NAC) for promoting osteodifferentiation.ResultsThe Gel-Ty hydrogels containing G-NAC (Gel-Ty/G-NAC) had suitable mechanical strength and biocompatibility to embed and support the growth of human adipose derived stem cells (hASCs) during a proliferation test for three days. In addition, G-NAC promoted osteodifferentiation both when it was included in Gel-Ty and when it was used directly in hASCs. The osteogenic effects were demonstrated by the alkaline phosphatase (ALP) activity test.ConclusionThese findings indicate that the phenol crosslinking reaction is suitable for injectable hydrogels for tissue regeneration and G-NAC stimulate bone regeneration. Based on our results, we suggest that Gel-Ty/G-NAC hydrogels can serve both as a biodegradable graft material for bone defect treatment and as a good template for tissue engineering applications such as drug delivery, cell delivery, and various tissue regeneration uses.

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Section: Discussionmentioning

confidence: 99%

Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

Lee

Heo

Nah

et al. 2018

IJN

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“…GNPs have been proven to be a better choice for in vivo and in vitro imaging due to low toxicity, low interaction with biological components, easy synthesis, easy surface modication, and easy LSPR tunability. 222,223 Based on the unique photo-physical properties of GNPs, many researchers have developed numerous new methodologies (e.g., optical coherence tomography) for incorporation of gold nanostructures in bioimaging. 224 Two photon luminescence microscopy is another popular bio-imaging technique which overcomes the difficulties faced by other optical imaging methods such as auto-uorescence of biological tissues.…”

Section: Bio-imaging Applicationsmentioning

confidence: 99%

Role of gold nanoparticles in advanced biomedical applications

et al. 2020

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“…Full discussion of this group of surface modification techniques exceeds the scope of this review. Notably, significant recent advances have been made in polyphenol and polydopamine coating systems (Lee et al, 2018;Xu, Neoh, & Kang, 2018).…”

Section: Figurementioning

confidence: 99%

Tuning the biomimetic behavior of scaffolds for regenerative medicine through surface modifications

Richbourg

Peppas

Sikavitsas

2019

J Tissue Eng Regen Med

150

115

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Tissue engineering and regenerative medicine rely extensively on biomaterial scaffolds to support cell adhesion, proliferation, and differentiation physically and chemically in vitro and in vivo. Changes to the surface characteristics of the scaffolds have the greatest impact on cell response. Here, we discuss five dominant surface modification approaches used to biomimetically improve the most common scaffolds for tissue engineering, those based on aliphatic polyesters. Scaffolds of aliphatic polyesters such as poly(L-lactic acid), poly(L-lactic-co-glycolic acid), and poly(ε-caprolactone) are often used in tissue engineering because they provide desirable, tunable properties such as ease of manufacturing, good mechanical properties, and nontoxic degradation products. However, cell–surface interactions necessary for tissue engineering are limited on these materials by their smooth postfabrication surfaces, hydrophobicity, and lack of recognizable biochemical binding sites. The surface modification techniques that have been developed for synthetic polymer scaffolds reduce initial barriers to cell adhesion, proliferation, and differentiation. Topographical modification, protein adsorption, mineral coating, functional group incorporation, and biomacromolecule immobilization each contribute through varying mechanisms to improving cell interactions with aliphatic polyester scaffolds. Furthermore, rational combination of methods from these categories can provide nuanced, specific environments for targeted tissue development.

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In situgold nanoparticle growth on polydopamine-coated 3D-printed scaffolds improves osteogenic differentiation for bone tissue engineering applications:in vitroandin vivostudies

Cited by 78 publications

References 58 publications

Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

Role of gold nanoparticles in advanced biomedical applications

Tuning the biomimetic behavior of scaffolds for regenerative medicine through surface modifications

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