<i>Ilheus Virus</i>Isolate from a Human, Ecuador

Johnson, Barbara W.; Cruz, Cristopher; Felices, Vidal; Espinoza, William R.; Manock, Stephen R.; Guevara, Carolina; Olson, James G.; Kochel, Tadeusz J.

doi:10.3201/eid1306.070118

Cited by 28 publications

(37 citation statements)

References 8 publications

(9 reference statements)

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“…A lesser proportion of patients were found to be acutely infected with Rickettsiae , dengue virus, and Coxiella . Testing for Ilhéus 16 and St. Louis encephalitis viruses became available late in the study. Although these pathogens were detected in less than 1% of participants overall, 27.3% of the 11 patients tested were positive for Ilhéus and 9.1% were positive for St. Louis encephalitis.…”

Section: Discussionmentioning

confidence: 99%

Etiology of Acute Undifferentiated Febrile Illness in the Amazon Basin of Ecuador

Manock¹,

Jacobsen²,

Bravo³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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115

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We conducted a longitudinal observational study of 533 patients presenting to two hospitals in the Ecuadorean Amazon basin with acute undifferentiated febrile illness (AUFI) from 2001 through 2004. Viral isolation, reverse transcription-polymerase chain reaction (RT-PCR), IgM seroconversion, and malaria smears identified pathogens responsible for fever in 122 (40.1%) of 304 patients who provided both acute and convalescent blood samples. Leptospirosis was found in 40 (13.2%), malaria in 38 (12.5%), rickettsioses in 18 (5.9%), dengue fever in 16 (5.3%), Q fever in 15 (4.9%), brucellosis in 4 (1.3%), Ilhéus infection in 3 (1.0%), and Venezuelan equine encephalitis (VEE), Oropouche, and St. Louis encephalitis virus infections in less than 1% of these patients. Viral isolation and RT-PCR on another 229 participants who provided only acute samples identified 3 cases of dengue fever, 2 of VEE, and 1 of Ilhéus. None of these pathogens, except for malaria, had previously been detected in the study area.

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Section: Discussionmentioning

confidence: 99%

Etiology of Acute Undifferentiated Febrile Illness in the Amazon Basin of Ecuador

Manock¹,

Jacobsen²,

Bravo³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

Self Cite

115

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“…The NCBI file with the highest identity was the Ilheus virus isolate PE163615 (GenBank: EF396950.1) and had an e-value of 0.0, a maximum score of 1688, coverage of 95%, and a maximum identity of 98%.The complete genome (10,759-bp) was sequenced and the alignment with the only full-length genome available in GenBank revealed a 95% similarity to the original strain of ILHV, isolated in Brazil in 1944 (NCBI Reference Sequence: NC_009028) (Figure 2). Phylogenetic analysis grouped the NS5 and Envelope sequences of our isolate with Ilheus virus isolate FSE800 (GenBank: EF396947.1) (Figure 3) isolated from a human case in Ecuador [11]. A total of 44 nonsynonymous amino acid changes were identified between our isolate and the GenBank Ilheus 1944 isolate.…”

Section: Resultsmentioning

confidence: 93%

“…ILHV causes mainly asymptomatic infections in humans with rare reports of encephalitis throughout northern South America [4], [5]. Human infection with ILHV has been reported in Trinidad, Panama, Colombia, French Guyana, Brazil, Ecuador and Bolivia [6], [7], [8], [9], [10], [11], [12].…”

Section: Introductionmentioning

confidence: 99%

Ilheus Virus Isolation in the Pantanal, West-Central Brazil

et al. 2013

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The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal.

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“…In Brazil, it has been isolated also from birds, sentinel monkeys and horses (Iversson et al 1993). Only a few reports of isolation from humans are available (Johnson et al 2007;Spence et al 1962;Srihongse and Johnson 1967;Venegas et al 2012). Results of infection are widely variable, ranging from asymptomatic to encephalitis, but most of the cases are accompanied by fever, headache, chills, photophobia, arthralgia, myalgia and asthenia (da Silva Azevedo et al 2010).…”

Section: Flaviviridae: Flavivirusmentioning

confidence: 99%