Î³Î´ T Lymphocytes in the Diagnosis of Human T Cell Receptor Immunodeficiencies

Garcillán, Beatriz; Marín, Ana V.; Jiménez-Reinoso, Anaïs; Briones, Alejandro C.; Muñoz-Ruiz, Miguel; García-León, María J.; Gil, Juana; Allende, Luis M.; Martı́nez-Naves, Eduardo; Toribio, Marı́a L.; Regueiro, José R.

doi:10.3389/fimmu.2015.00020

Cited by 47 publications

(38 citation statements)

References 35 publications

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“…Tgd17 cells can be positive for CD4 and IL-17A, so they may also be responsible for inducing syndecan-2 expression. However, about 70% of gdT cells exist as CD4 2 CD8 2 cells, and they only account for about 1-10% of blood cells (42)(43)(44). The markers consistently used to identify gdT cells are Vg4 high , CCR6 high , and CD27 low , which we did not use to determine specific immune cells in our experiments.…”

Section: Discussionmentioning

confidence: 92%

“…Acute colitis models were produced in mice by oral administration of 1.5 or 3% DSS [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50] in the drinking water for 4 d followed by 6 d on tap water. For acetylsalicylic acid (ASA) treatment, mice were divided into 3 groups, which were treated as follows: 3% DSS treatment plus daily injections of 0.5 mg, i.p.…”

Section: The Acute Dss-induced Murine Model Of Colitismentioning

confidence: 99%

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Up‐regulation of syndecan‐2 in proximal colon correlates with acute inflammation

et al. 2019

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We previously reported that syndecan‐2 expression is increased on the colonic epithelium during chronic inflammation. Here, we report that syndecan‐2 exhibits a different pattern of site‐specific colonic expression during acute inflammation. Syndecan‐2 expression was up‐regulated predominantly in the proximal colon of dextran sulfate sodium‐induced colitis mice. The colitis‐associated up‐regulation of syndecan‐2 was barely detected in Rag‐1−/− (recombination activating gene 1 knockout) mice under colitis‐inducing conditions. Increased syndecan‐2 expression correlated with increased levels of infiltrated CD4+IL‐17A+ T cells in the proximal colon. Serum levels of IL‐17A were increased during the acute inflammatory response in normal mice but not Rag‐1−/− mice. IL‐17A directly induced IL‐17 receptor (IL‐17RA) and syndecan‐2 expression in ex vivo‐cultured proximal colon tissues and adenoma cell lines from proximal colon. IL‐17RA knockdown reduced the IL‐17A‐mediated syndecan‐2 expression in SNU1235 cells. No elevation of syndecan‐2 or IL‐17RA was observed in colonic tissues from IL‐17A−/− mice during colitis induction. Finally, increased expression of syndecan‐2 and IL‐17RA was observed in the proximal colons of cecal ligation and puncture‐induced sepsis mice and infectious pan colitis patients. Together, these data suggest that acute inflammation induces syndecan‐2 expression predominantly in the proximal colon via IL‐17A‐IL‐17RA signaling during the early stage of the inflammatory response and that proximal colonic syndecan‐2 might be a biomarker for acute inflammation.—Hong, H., Song, H.‐K., Hwang, E. S., Lee, A. R., Han, D. S., Kim, S.‐E., Oh, E.‐S. Up‐regulation of syndecan‐2 in proximal colon correlates with acute inflammation. FASEB J. 33, 11381–11395 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 92%

Section: The Acute Dss-induced Murine Model Of Colitismentioning

confidence: 99%

Up‐regulation of syndecan‐2 in proximal colon correlates with acute inflammation

et al. 2019

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“…In addition, interestingly, there are six clusters of CD4-CD8-T cells, where four are γδ T cells. These have been noted as a characteristic of reaction to viral infections [35][36][37][38][39]. There are twelve clusters representing effector memory cells and nine regulatory T cells that are CD4+Foxp3+.…”

Section: Differential Cluster Proportions Between Subjectsmentioning

confidence: 99%

Exploring Single-Cell Data with Deep Multitasking Neural Networks

Amodio

Dijk

Srinivasan

et al. 2017

Preprint

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Handling the vast amounts of single-cell RNA-sequencing and CyTOF data, which are now being generated in patient cohorts, presents a computational challenge due to the noise, complexity, sparsity and batch effects present. Here, we propose a unified deep neural network-based approach to automatically process and extract structure from these massive datasets. Our unsupervised architecture, called SAUCIE (Sparse Autoencoder for Unsupervised Clustering, Imputation, and Embedding), simultaneously performs several key tasks for single-cell data analysis including 1) clustering, 2) batch correction, 3) visualization, and 4) denoising/imputation. SAUCIE is trained to recreate its own input after reducing its dimensionality in a 2-D embedding layer which can be used to visualize the data. Additionally, it uses two novel regularizations: (1) an information dimension regularization to penalize entropy as computed on normalized activation values of the layer, and thereby encourage binary-like encodings that are amenable to clustering and (2) a Maximal Mean Discrepancy penalty to correct batch effects. Thus SAUCIE has a single architecture that denoises, batch-corrects, visualizes and clusters data using a unified 1 . CC-BY 4.0 International license peer-reviewed) is the author/funder. It is made available under a

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“…TCR is a heterodimer formed of either α and β (in over 90% of T cells) or γ and δ chains (in 1-10% of T cells) ( Figure 1a) [5,6]. TCR lacks its own signalling domains, therefore it associates with CD3 complex that consists of two heterodimers CD3γ/CD3ε and CD3δ/CD3ε and a single homodimer CD3ζ/CD3ζ (also termed CD247).…”

Section: Tcr-structure and Signallingmentioning

confidence: 99%

Adoptive Cell Therapy—Harnessing Antigen-Specific T Cells to Target Solid Tumours

Chruściel

Urban-Wójciuk

Arcimowicz

et al. 2020

Cancers

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In recent years, much research has been focused on the field of adoptive cell therapies (ACT) that use native or genetically modified T cells as therapeutic tools. Immunotherapy with T cells expressing chimeric antigen receptors (CARs) demonstrated great success in the treatment of haematologic malignancies, whereas adoptive transfer of autologous tumour infiltrating lymphocytes (TILs) proved to be highly effective in metastatic melanoma. These encouraging results initiated many studies where ACT was tested as a treatment for various solid tumours. In this review, we provide an overview of the challenges of T cell-based immunotherapies of solid tumours. We describe alternative approaches for choosing the most efficient T cells for cancer treatment in terms of their tumour-specificity and phenotype. Finally, we present strategies for improvement of anti-tumour potential of T cells, including combination therapies.

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Î³Î´ T Lymphocytes in the Diagnosis of Human T Cell Receptor Immunodeficiencies

Cited by 47 publications

References 35 publications

Up‐regulation of syndecan‐2 in proximal colon correlates with acute inflammation

Up‐regulation of syndecan‐2 in proximal colon correlates with acute inflammation

Exploring Single-Cell Data with Deep Multitasking Neural Networks

Adoptive Cell Therapy—Harnessing Antigen-Specific T Cells to Target Solid Tumours

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