<i>I‐SceI</i> meganuclease‐mediated transgenesis in <i>Xenopus</i>

Pan, Fong Cheng; Chen, Yonglong; Loeber, Jana; Henningfeld, Kristine A.; Pieler, Tomas

doi:10.1002/dvdy.20608

Cited by 112 publications

(99 citation statements)

References 23 publications

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“…Most important to the field was the establishment of a reliable transgenic method that is based on restriction enzyme mediated gene integration (REMI) and sperm nuclear transfer into eggs (Kroll and Amaya, 1996). More recently, the method has been simplified (Sparrow et al, 2000) and a second method using SceI meganuclease has also been introduced (Ogino et al, 2006;Pan et al, 2006). Either method allows the creation of 50 -100 founder transgenics in 1 day.…”

Section: Candidate Gene Approaches Using Topical Application and Tranmentioning

confidence: 99%

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Beck

Belmonte

Christen

2009

Developmental Dynamics

146

139

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While Xenopus is a well-known model system for early vertebrate development, in recent years, it has also emerged as a leading model for regeneration research. As an anuran amphibian, Xenopus laevis can regenerate the larval tail and limb by means of the formation of a proliferating blastema, the lens of the eye by transdifferentiation of nearby tissues, and also exhibits a partial regeneration of the postmetamorphic froglet forelimb. With the availability of inducible transgenic techniques for Xenopus, recent experiments are beginning to address the functional role of genes in the process of regeneration. The use of soluble inhibitors has also been very successful in this model. Using the more traditional advantages of Xenopus, others are providing important lineage data on the origin of the cells that make up the tissues of the regenerate. Finally, transcriptome analyses of regenerating tissues seek to identify the genes and cellular processes that enable successful regeneration.

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Section: Candidate Gene Approaches Using Topical Application and Tranmentioning

confidence: 99%

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Beck

Belmonte

Christen

2009

Developmental Dynamics

146

139

View full text Add to dashboard Cite

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“…4 O and P). The same effect can also be visualized by using stage 47 embryos in elastase:GFP transgenic frogs generated in our laboratory (33); the number of GFP-positive cells, reflecting activation of exocrine-specific elastase, was several times higher in the hhex-induced giant ventral pancreata than in the pancreas of control embryos (Fig. 4 Q and R).…”

Section: Hhex-induced Expansion Of the Ventral Pancreas Is Associatedmentioning

confidence: 63%

Homeoprotein hhex-induced conversion of intestinal to ventral pancreatic precursors results in the formation of giant pancreata in Xenopus embryos

Zhao

Han

Dawid

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Liver and ventral pancreas develop from neighboring territories within the endoderm of gastrulae. ventral pancreatic precursor 1 (vpp1) is a marker gene that is differentially expressed in a cell population within the dorsal endoderm in a pattern partially overlapping with that of hematopoietically expressed homeobox (hhex) during gastrulation. In tail bud embryos, vpp1 expression specifically demarcates two ventral pancreatic buds, whereas hhex expression is mainly restricted to the liver diverticulum. Ectopic expression of a critical dose of hhex led to a greatly enlarged vpp1-positive domain and, subsequently, to the formation of giant ventral pancreata, putatively by conversion of intestinal to ventral pancreatic precursor cells. Conversely, antisense morpholino oligonucleotidemediated knockdown of hhex resulted in a down-regulation of vpp1 expression and a specific loss of the ventral pancreas. Furthermore, titration of hhex with a dexamethasone-inducible hhex-VP16GR fusion construct suggested that endogenous hhex activity during gastrulation is essential for the formation of ventral pancreatic progenitor cells. These observations suggest that, beyond its role in liver development, hhex controls specification of a vpp1-positive endodermal cell population during gastrulation that is required for the formation of the ventral pancreas.endoderm regionalization | endocrine | exocrine | proliferation | apoptosis

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“…Obviously, prompt processing of tadpole tissue is required for such retrospective analyses to succeed, as cells that express M2(H37A) will be targeted for destruction. In the future, the advent of new amphibian transgenesis procedures may lessen the difficulties of transgene expression variability (Allen and Weeks, 2005;Ogino et al, 2006;Pan et al, 2006).…”

Section: Targeting Of M2(h37a) Expression Using Transgenesis In Xenopusmentioning

confidence: 99%

Targeted cell‐ablation in Xenopus embryos using the conditional, toxic viral protein M2(H37A)

Smith¹,

Kotecha²,

Towers³

et al. 2007

Developmental Dynamics

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Harnessing toxic proteins to destroy selective cells in an embryo is an attractive method for exploring details of cell fate and cell-cell interdependency. However, no existing "suicide gene" system has proved suitable for aquatic vertebrates. We use the M2(H37A) toxic ion channel of the influenza-A virus to induce cell-ablations in Xenopus laevis. M2(H37A) RNA injected into blastomeres of early stage embryos causes death of their progeny by late-blastula stages. Moreover, M2(H37A) toxicity can be controlled using the M2 inhibitor rimantadine. We have tested the ablation system using transgenesis to target M2 (

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I‐SceI meganuclease‐mediated transgenesis in Xenopus

Cited by 112 publications

References 23 publications

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Homeoprotein hhex-induced conversion of intestinal to ventral pancreatic precursors results in the formation of giant pancreata in Xenopus embryos

Targeted cell‐ablation in Xenopus embryos using the conditional, toxic viral protein M2(H37A)

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