<i>Hypericum</i>in infection: Identification of anti-viral and anti-inflammatory constituents

Birt, Diane F.; Widrlechner, Mark P.; Hammer, Kimberly D.P.; Hillwig, Matthew L.; Wei, Jingqiang; Kraus, George A.; Murphy, Patricia A.; McCoy, Joe Ann; Wurtele, Eve Syrkin; Neighbors, Jeffrey D.; Wiemer, David F.; Maury, Wendy; Price, John D.

doi:10.1080/13880200902988645

Cited by 70 publications

(48 citation statements)

References 31 publications

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“…Depressive disorders are the best-known indication for SJW and preclinical and clinical investigations have showed its efficacy against mild to moderate depression with a favorable incidence of side effects than standard antidepressants (Kasper et al 2010). In addition to the antidepressant activity, some studies have documented other bioactivities produced by SJW as antibacterial (Saddiqe et al 2010) and antiviral (Birt et al 2009) activities. SJW is also involved in pain processing.…”

Section: Introductionmentioning

confidence: 99%

Reversal of NO-induced nociceptive hypersensitivity by St. John’s wort and hypericin: NF-κB, CREB and STAT1 as molecular targets

Galeotti¹,

Ghelardini²

2012

Psychopharmacology

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Rationale Hypericum perforatum, popularly called St. John's wort (SJW), is a medicinal plant mainly used as antidepressant with a favorable safety profile than standard antidepressants. Some studies have also documented other SJW bioactivities, including pain modulation. Objectives The aim of this study was to demonstrate the capability of SJW to relieve nitric oxide (NO)-induced nociceptive hypersensitivity and identify the effective component. Methods Nociceptive hypersensitivity induced by administration of the NO donors nitroglycerin (GTN) and sodium nitroprusside (SNP) was assessed by cold and hot plate tests. The cellular pathways and molecular targets involved were investigated by Western blotting. Results GTN and SNP produced a prolonged allodynia and hyperalgesia in mice. A single oral administration of low doses of an SJW dried extract or purified hypericin reversed the NO donor-induced nociceptive behavior whereas hyperforin and flavoinoids were ineffective. Investigating into the cellular pathways involved, an increased CREB and STAT1 phosphorylation, and activation of NF-κB were detected within PAG and thalamus following NO donors' administration. These cellular events were prevented by SJW or hypericin. Since hypericin showed PKC blocking properties, a role of PKC as an upstream modulator of these transcription factors was hypothesized. NO donors increased expression and phosphorylation of protein kinase C (PKC) γ and ε isoforms, molecular events prevented by SJW or hypericin. Conclusions SJW reversed NO-induced nociceptive hypersensitivity through the blockade of a supraspinal signaling pathway involving a PKC-dependent CREB, STAT1 and NF-κB activation due to presence of hypericin. These data indicate SJW/hypericin as a therapeutic perspective for pain treatment.

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Section: Introductionmentioning

confidence: 99%

Reversal of NO-induced nociceptive hypersensitivity by St. John’s wort and hypericin: NF-κB, CREB and STAT1 as molecular targets

Galeotti¹,

Ghelardini²

2012

Psychopharmacology

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“…11,12 Although, H. perforatum has been reported in a wide variety of metabolites, few chemical investigations of the adventitious roots of H. perforatum have been reported. In this report, a new compound (1) and 11 known compounds (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) were isolated from a methanol extract of adventitious roots of H. perforatum.…”

Section: Introductionmentioning

confidence: 99%

“…4,5 Presently, fieldgrown plant material is generally used, but the quality of these products may be greatly affected by various environmental conditions, fungi, bacteria, viruses, and insects, which can result in heavy loss of yield and alter the medicinal content of plants. 6 Therefore, cell or organ cultures have emerged as valuable routes for biosynthesizing phytochemicals.…”

Section: Introductionmentioning

confidence: 99%

“…These fractions were subjected to various separation procedures and 12 compounds were isolated ( Figure 1). Structures of known compounds (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) were elucidated by comparing spectroscopic data to published values and identified as acetylannulatophenonoside (2), 22 1,5,6-trihydroxy-3-methoxyxanthone (3), 23 1,3,5,6-tetrahydroxyxanthone (4), 24 ferrxanthone (5), 25 brasilixanthone B (6), 26 neolancerin (7), 31 and glucosyringic acid (12). 32 Of these, compounds 2, 5-7, 10, and 11 were isolated from this plant for the first time.…”

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confidence: 99%

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NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

Li¹,

Ding²,

Quang³

et al. 2013

Bulletin of the Korean Chemical Society

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A new compound, perforaphenonoside A (1), along with 11 known compounds (2-12) were isolated from a methanol extract of adventitious roots of Hypericum perforatum. Their chemical structures were elucidated using chemical and physical methods as well as comparison of NMR and mass spectral data with previously reported data. Their inhibition of NF-κB and activation of PPAR was measured in HepG2 cells using a luciferase reporter system. Among the compounds 3, 6, 7 and 12 inhibited NF-κB activation stimulated by TNFα in a dose-dependent manner, with IC 50 values ranging from 0.85 to 8.10 μM. Moreover, compounds 1-3, 7, 11 and 12 activated the transcriptional activity of PPARs in a dose-dependent manner, with EC 50 values ranging from 7.3 to 58.7 μM. The transactivational effects of compounds 1-3, 7, 11 and 12 were evaluated on three individual PPAR subtypes. Among them, compound 2 activated PPARα transcriptional activity, with 153.97% stimulation at 10 μM, while compounds 1, 2 and 11 exhibited transcriptional activity of PPARγ, with stimulation from 124.76% to 126.91% at 10 μM.

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“…Hypericum perforatum L. (St. John's Wort) is a perennial flowering plant that has been used for centuries as a natural remedy for the treatment of a variety of disorders [1,2]. Hypericum perforatum extracts contain several compounds with biological activity such as hypericin, pseudohypericin, flavonoids, oligomeric procyanidins and hyperforin [3].…”

Section: Introductionmentioning

confidence: 99%

Effects of Hypericum perforatum extract on oxaliplatin-induced neurotoxicity: in vitro evaluations

Cinci

Mannelli

Maidecchi

et al. 2017

Zeitschrift Für Naturforschung C

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Hypericum perforatum L. has been used for centuries as a natural remedy for the treatment of many disorders. Neuropathic pain is a common side effect of oxaliplatin-based chemotherapy and often the cause of therapy discontinuation. Thanks to its anti-inflammatory and analgesic effects, the use of H. perforatum may be a novel therapeutic strategy for neuropathy. The aim of this paper was to evaluate the effect of H. perforatum hydrophilic extract on an in vitro model of oxaliplatin-induced neurotoxicity. The antioxidant potential of extract was first evaluated in cell-free models by the thiobarbituric acid-reactive substances assay and nitro blue tetrazolium oxidation test; the ability of H. perforatum extract to reduce oxaliplatin-induced caspase-3 activity in rat astrocytes and its potential interference with the cytotoxic effects of oxaliplatin in a colorectal cancer in vitro model (HT-29 cells) were also evaluated. The extract showed a significant antioxidant effect and was able to reduce caspase-3 activity in rat astrocytes. Of note, the extract alone exerted a cytotoxic effect in HT-29 cells and did not reduce the cytotoxicity of oxaliplatin in HT-29 cells. These data suggest that H. perforatum could be used as a novel therapeutic strategy for counteracting chemotherapy-induced neuropathy.

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Hypericumin infection: Identification of anti-viral and anti-inflammatory constituents

Cited by 70 publications

References 31 publications

Reversal of NO-induced nociceptive hypersensitivity by St. John’s wort and hypericin: NF-κB, CREB and STAT1 as molecular targets

Reversal of NO-induced nociceptive hypersensitivity by St. John’s wort and hypericin: NF-κB, CREB and STAT1 as molecular targets

NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

Effects of Hypericum perforatum extract on oxaliplatin-induced neurotoxicity: in vitro evaluations

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