<i>Holothuria scabra</i>extracts exhibit anti-Parkinson potential in<i>C. elegans</i>: A model for anti-Parkinson testing

Chalorak, Pawanrat; Jattujan, Prapaporn; Nobsathian, Saksit; Poomtong, Tanate; Sobhon, Prasert; Meemon, Krai

doi:10.1080/1028415x.2017.1299437

Cited by 54 publications

(50 citation statements)

References 41 publications

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“…degeneration of the DA neuron in C. elegans, including foodsensing behavior and the shortened lifespan. These results are consistent with the previous studies that have reported 6-OHDA-induced parkinsonism in both in vitro and in vivo models (Latchoumycandane et al, 2011;Bagga et al, 2015;Chalorak et al, 2018). 6-OHDA selectively induced the toxicity in the DA neurons by entering the cell through dopamine transporter which expressed exclusively in the DA neuronal cell surface (Nass et al, 2002).…”

Section: Discussionsupporting

confidence: 93%

Downregulation of eEF1A/EFT3-4 Enhances Dopaminergic Neurodegeneration After 6-OHDA Exposure in C. elegans Model

2020

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Parkinson's disease (PD) is a neurodegenerative disorder characterized by the aggregation of α-synuclein protein and selective death of dopaminergic (DA) neurons in the substantia nigra of the midbrain. Although the molecular pathogenesis of PD is not completely understood, a recent study has reported that eukaryotic translation elongation factor 1 alpha (eEF1A) declined in the PD-affected brain. Therefore, the roles of eEF1A1 and eEF1A2 in the prevention of DA neuronal cell death in PD are aimed to be investigated. Herein, by using Caenorhabditis elegans as a PD model, we investigated the role of eft-3/eft-4, the worm homolog of eEF1A1/eEF1A2, on 6-hydroxydopamine (6-OHDA)-induced DA neuron degeneration. Our results demonstrated that the expressions of eft-3 and eft-4 were decreased in the 6-OHDA-induced worms. RNA interference (RNAi) of eft-3 and eft-4 resulted in dramatic exacerbation of DA neurodegeneration induced by 6-OHDA, as well as aggravated the food-sensing behavior, ethanol avoidance, and decreased lifespan when compared with only 6-OHDA-induced worms. Moreover, downregulation of eft-3/4 in 6-OHDA-induced worms suppressed the expression of the anti-apoptotic genes, including PI3K/age-1, PDK-1/pdk-1, mTOR/let-363, and AKT-1,2/akt-1,2, promoting the expression of apoptotic genes such as BH3/egl-1 and Caspase-9/ced-3. Collectively, these findings indicate that eEF1A plays an important role in the 6-OHDA-induced neurodegeneration through the phosphatidylinositol 3-kinase (PI3K)/serine/threonine protein kinase (Akt)/mammalian target of rapamycin (mTOR) pathway and that eEF1A isoforms may be a novel and effective pro-survival factor in protective DA neurons against toxin-induced neuronal death.

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Section: Discussionsupporting

confidence: 93%

Downregulation of eEF1A/EFT3-4 Enhances Dopaminergic Neurodegeneration After 6-OHDA Exposure in C. elegans Model

2020

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“…Whole body-ethyl acetate (WBEA), whole body-butanol (WBBU), and body wall-ethyl acetate (BWEA) ( 27 ) are extracts of sea cucumber Holothuria scabra . These compounds could significantly attenuate the degeneration of DA neurons induced by 6-OHDA in caenorhabditis elegans [68]. Moreover, these extracts could inhibit abnormal aggregation of α-synuclein, and restore lipid content [68].…”

Section: Potential Candidates From Marine-derived Compounds For Thmentioning

confidence: 99%

Marine-Derived Natural Compounds for the Treatment of Parkinson’s Disease

2019

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Parkinson’s disease (PD) is a neurodegenerative disorder caused by the loss of dopaminergic neurons, leading to the motor dysfunctions of patients. Although the etiology of PD is still unclear, the death of dopaminergic neurons during PD progress was revealed to be associated with the abnormal aggregation of α-synuclein, the elevation of oxidative stress, the dysfunction of mitochondrial functions, and the increase of neuroinflammation. However, current anti-PD therapies could only produce symptom-relieving effects, because they could not provide neuroprotective effects, stop or delay the degeneration of dopaminergic neurons. Marine-derived natural compounds, with their novel chemical structures and unique biological activities, may provide anti-PD neuroprotective effects. In this study, we have summarized anti-PD marine-derived natural products which have shown pharmacological activities by acting on various PD targets, such as α-synuclein, monoamine oxidase B, and reactive oxygen species. Moreover, marine-derived natural compounds currently evaluated in the clinical trials for the treatment of PD are also discussed.

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“…Triterpene glycosides or saponins have been found to be major constituents in the n-butanol fractions that may act in the fat metabolism. [18][19][20] Similarly, a previous study reported that triglyceride accumulation was suppressed and delayed in rats fed with high-fat diet by saponins isolated from the temperate sea cucumber, Cucumaria frondosa. 21 We further found that the effectiveness of both BW and CT extracts in decreasing of fat accumulation and triglyceride levels were similar, which implied that BW and CTs possess similar groups of bioactive saponins.…”

Section: Discussionmentioning

confidence: 83%

“…These results indicated that the bioactive compounds that reduced fat and triglyceride levels were concentrated in the n ‐butanol fraction. Triterpene glycosides or saponins have been found to be major constituents in the n ‐butanol fractions that may act in the fat metabolism . Similarly, a previous study reported that triglyceride accumulation was suppressed and delayed in rats fed with high‐fat diet by saponins isolated from the temperate sea cucumber, Cucumaria frondosa .…”

Section: Discussionmentioning

confidence: 92%

Saponin‐enriched extracts from body wall and Cuvierian tubule of Holothuria leucospilota reduce fat accumulation and suppress lipogenesis in Caenorhabditis elegans

et al. 2019

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BACKGROUND Saponins have been shown to possess many pharmacological properties, including altered fat metabolism. The black sea cucumber, Holothuria leucospilota, is a marine animal that contains a specialized organ called a Cuvierian tubule that produces and secrete the bioactive saponins into the tubules and body wall. Therefore, the aims of this study are to investigate the anti‐obesity effect of saponins extracted from body wall and Cuvierian tubules of H. leucospilota. RESULTS The butanol extracts of H. leucospilota body wall and Cuvierian tubules containing high amounts of saponins significantly reduced fat deposition and triglyceride levels in Caenorhabditis elegans fed with 50 mmol L−1 glucose. Moreover, the saponin‐enriched extracts of H. leucospilota significantly restored the lifespan of 2% glucose‐fed worms (18.71%). Green fluorescence protein‐labeled sbp‐1 gene expression and nuclear translocation of daf‐16 were also significantly decreased in H. leucospilota treatment. The saponin‐enriched extracts downregulated the messenger RNA expressions of genes involved in fat storage and metabolism, including sbp‐1, cebp, and daf‐16 but upregulated the expression of nhr‐49 gene. CONCLUSION Our results suggest that H. leucospilota‐derived saponins may mediate the reduction of glucose‐induced fat accumulation through sbp‐1, cebp, daf‐16 and nhr‐9 pathways. Therefore, the H. leucospilota extracts could be used as nutraceuticals for anti‐obesity prevention. © 2019 Society of Chemical Industry

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Holothuria scabraextracts exhibit anti-Parkinson potential inC. elegans: A model for anti-Parkinson testing

Cited by 54 publications

References 41 publications

Downregulation of eEF1A/EFT3-4 Enhances Dopaminergic Neurodegeneration After 6-OHDA Exposure in C. elegans Model

Downregulation of eEF1A/EFT3-4 Enhances Dopaminergic Neurodegeneration After 6-OHDA Exposure in C. elegans Model

Marine-Derived Natural Compounds for the Treatment of Parkinson’s Disease

Saponin‐enriched extracts from body wall and Cuvierian tubule of Holothuria leucospilota reduce fat accumulation and suppress lipogenesis in Caenorhabditis elegans

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