<i>Helicobacter pylori</i> infection of gastric mucin cell metaplasia: The duodenum revisited

Johan, G.; Offerhaus, A.; En, Molyvas; Pj, Hoedemaeker

doi:10.1002/path.1711620310

Cited by 18 publications

(8 citation statements)

References 14 publications

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“…Thus, our adhesive strains may adhere to human gastric antral epithelial cells but not to fundic cells. This putative target cell specificity corresponds well to the fact that H. pylon is often observed to adhere to the antral epithelium of human stomach and the gastric metaplasia in the duodenum (17,26,30,33). Thus, the primary colonization site ofH.…”

Section: Discussionsupporting

confidence: 72%

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Adhesion of Helicobacter pylori to gastric epithelial cells in primary cultures obtained from stomachs of various animals

1993

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Of 35 strains of Helicobacter pyloni tested, 5 were found to adhere well to HEp-2 cells. We selected three of these adhesive strains and four from the remaining strains to examine their ability to adhere to gastric epithelial cells in primary cultures obtained by collagenase digestion of stomachs from mice, rats, Mongolian gerbils, guinea pigs, pigs, and cynomolgus monkeys. The three adhesive strains adhered well to epithelial cells from monkey and pig gastric antra. The adhesion was inhibited by incubating the bacterial cells with fetuin, and this inhibition was further confirmed by the binding of gold-labeled fetuin to the surface of the adhesive strains. However, these adhesive strains only weakly adhered to fundic epithelial cells from monkeys and pigs and to gastric epithelial cells from the other animals. As for the four strains poorly adhesive to HEp-2 cells, they adhered weakly to gastric epithelial cells from all of the animals tested. They had higher hemagglutination titers than the adhesive strains, showing that there was no correlation between hemagglutination titers and the ability to adhere to gastric cells. Taking the similarities of human and monkey or pig stomachs into consideration, these results suggest that the primary target cell of H. pylon in colonization in human stomachs is the antral epithelial cell and that the putative adhesin involved in adhesion has affinity for fetuin.

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Section: Discussionsupporting

confidence: 72%

“…Helicobacter pyloni is a microaerophilic spiral bacterium that was isolated from the gastric mucosa of patients with chronic gastritis in 1983 (36). H. pylori colonizes the gastric epithelial cell surface of the human stomach and the gastric metaplasia in the duodenum (17,26,30,33). Infection by this organism is very common in the human population.…”

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confidence: 99%

Adhesion of Helicobacter pylori to gastric epithelial cells in primary cultures obtained from stomachs of various animals

1993

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“…All strains consistently showed adhesion to human SMC while only one strain showed additional adhesion to bovine SMC. Outside the stomach, H. pylori has been found only in gastric metaplastic or heterotopic tissue, [1][2][3][4]6]. These cells are not related to the tissue which is infected by the bacteria in vivo.…”

Section: H Pylori Adhesion To Smc 457 Discussionmentioning

confidence: 99%

Adhesion of Helicobacter pylori and Escherichia coli to human and bovine surface mucus cells in vitro

Nilius

Bode

Büchler

et al. 1994

Eur J Clin Investigation

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Helicobacter pylori shows in vivo a specific affinity for epithelial surface mucus cells (SMC) of the human stomach. We studied the in vitro adhesion of five different H. pylori strains and one non-pathogenic Escherichia coli-strain to (a) human antral SMC, obtained during gastroscopy; (b) human tumour SMC, from a carcinoma cell line (CRL 1739 AGS); and (c) bovine SMC, obtained from the abomasum. SMC of different origin were characterized by means of electron microscopy and immunohistochemistry, and showed similar main features: all cells showed intra-cellular structures like zymogens and PAS-positive mucin granules. HSMC were antibody-positive against epithelial cell markers. All five H. pylori strains adhered to human SMC (HSMC) and tumour SMC (TSMC). Only one strain additionally adhered to bovine SMC (BSMC). No adhesion to any of these cells was observed with E. coli. Adhesion in vitro is characterized by a close membrane-to-membrane association between H. pylori and the target cells. This phenomenon suggests a specific receptor-ligand interaction.

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“…Mounting epidemiological evidence implicates chronic H. pylon gastritis in the development of gastric and duodenal ulcer disease (1,22,32,36,50) and also gastric carcinoma (10,29). H. pylon infection has a worldwide distribution; it is more prevalent in developing countries and in economically disadvantaged people in more developed countries (5,17,39).…”

mentioning

confidence: 99%

“…H. pylon infection has a worldwide distribution; it is more prevalent in developing countries and in economically disadvantaged people in more developed countries (5,17,39). Although little is known about the mechanism(s) by which H. pylon infection leads to gastroduodenal disease, it has been shown that this microaerophilic organism preferentially adheres to and colonizes mucus-secreting gastric epithelial cells (1,5,20,32,36). Like other bacteria which colonize mucosal epithelia, H. pylon adheres to sialic acidrich macromolecules that are exposed on the mucosal cell membrane (6).…”

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Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori

et al. 1993

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Gene hpaA, which codes for the receptor-binding subunit of the N-acetylneuraminyllactose-binding fibrillar hemagglutinin (NLBH) of Helicobacter pylori, was cloned and sequenced. The protein expressed by hpaA, designated HpaA, was identified as the adhesin subunit on the basis of its fetuin-binding activity and its reactivity with a polyclonal, monospecific rabbit serum prepared against NLBH purified from H. pylori. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and Western blots (immunoblots) showed that the cloned adhesin has the same molecular weight (20,000) as that found on H. pylori. Also, HpaA contains a short sequence of amino acids (KRTIQK) which are all either identical or functionally similar to those which compose the sialic acid-binding motif of Escherichia coli SfaS, K99, and CFA/I. Affinity-purified antibody specific for a 12-residue synthetic peptide that included this sequence blocked the hemagglutinating activity of H. pylori and was shown by immuno-gold electron microscopy to react with almost transparent material on unstained H. pylori cells, which is consistent with previous observations concerning the location and morphology of the NLBH.

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Helicobacter pylori infection of gastric mucin cell metaplasia: The duodenum revisited

Cited by 18 publications

References 14 publications

Adhesion of Helicobacter pylori to gastric epithelial cells in primary cultures obtained from stomachs of various animals

Adhesion of Helicobacter pylori to gastric epithelial cells in primary cultures obtained from stomachs of various animals

Adhesion of Helicobacter pylori and Escherichia coli to human and bovine surface mucus cells in vitro

Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori

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