<i>Helicobacter pylori</i>infection

Vandenplas, Yvan

doi:10.3748/wjg.v6.i1.20

Cited by 40 publications

(5 citation statements)

References 159 publications

(167 reference statements)

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“…H pylori is one of the most common bacteria worldwide found in more than 50% of human population [16] . The presence of H pylori is the main cause of several gastroduodenal diseases, including peptic ulcer [4,6] , gastric cancer [17] , and gastric MALT lymphoma [18] , and has been designated as class I carcinogen by the World Health Organization [19] .…”

Section: Discussionmentioning

confidence: 99%

Helicobacter infection in hepatocellular carcinoma tissue

Xuan

Li²,

Qiang³

et al. 2006

WJG

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Section: Discussionmentioning

confidence: 99%

Helicobacter infection in hepatocellular carcinoma tissue

Xuan

Li²,

Qiang³

et al. 2006

WJG

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“…It was found that this protein in H. pylori broth culture filtrates could cause formation of large vacuoles in the cytoplasm of cultured mammalian cells [7]. The vacuolating activity is increased by exposure to acidic pH [6]. The vacA gene shows an allelic variation in two regions [8].…”

Section: Introductionmentioning

confidence: 99%

“…Another set of multiplex PCRs was used to detect the mid region of the vacA gene (m1 and m2). Primer sequences are contained in Table 1 as previously described [6,16,17]. A singleplex PCR was used to detect the cagA gene.…”

Section: Multiplex Pcr For H Pylori Genotypingmentioning

confidence: 99%

“…The mid-region M has two different alleles m1 and m2 [9]. Strains with the gene s1/m1 have the highest level of cytotoxic activity and are associated with peptic ulcers, atrophic gastritis and gastric cancer, while the s2/m2 strains have non-toxic activity [6]. The bacterial strains with s1/m1 genotype produce higher amounts of toxin as compared with s1/m2 [7].…”

Section: Introductionmentioning

confidence: 99%

“…pylori produces a variety of virulence factors such as motility, urease, catalase, flagella, phospholipases and cytotoxins [5]. The vacuolating cytotoxin vacA protein encoded by the vacA gene is present in all strains but only expressed in about 50% of the strains [6]. It was found that this protein in H. pylori broth culture filtrates could cause formation of large vacuoles in the cytoplasm of cultured mammalian cells [7].…”

Section: Introductionmentioning

confidence: 99%

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Helicobacter pylori Pathogenicity Associated cagA and vacA Genotypes Among Nigerian Dyspeptic Patients

E¹,

Emmanuel²,

Eu³

2017

J Mol Biomark Diagn

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Objective: Although Helicobacter pylori infection is endemic in Nigeria, the specific genotypes that influence treatment responses are scarcely known. We aimed to determine the specific genotypes of H. pylori virulence genes, vacA and cagA in dyspeptic patients. Subjects and methods:Gastric biopsy samples were obtained from 80 dyspeptic patients referred for endoscopy. Genomic DNA was extracted from biopsies using the ReliaPrep DNA kit. H. pylori DNA was detected by a singleplex PCR based on the genus specific 16s rRNA gene. The vacA subtypes for the s1 and s2 regions and the m1 and m2 alleles were detected by allele specific multiplex PCR. The cagA gene was amplified by a singleplex PCR. Results:Of the 80 samples, 30 (37.5%) had abnormal mucosa which were chronic gastritis. The rest (62.5%) had normal mucosa lining. Of the 30 chronic gastritis cases, 22 (73%) had H. pylori infection as detected by 16s rRNA PCR. Only 2 (4%) of the normal mucosa cases had H. pylori infection. For the vacA genotypes, 79% of the H. pylori infections were s1c/m2 genotype, followed by 8% s1b/m2 genotype. Three different genotypes: s1c/m1/m2, s1c/s2/m2 and s1c/m1 occurred at 4% each. The most virulent vacA genotype, s1/m1 was only 8% while the least virulent vacA genotype s2/m2 was 4%. The moderate virulent vacA genotype, s1/m2 was the most prevalent (83%) in the Nigerian patients. The most prevalent subtype was the s1c/m2. Only 7 cases (29%) were cagA positive. Conclusion:The pathogenicity-associated virulence genes present in Nigerian dyspeptic patients were moderate types. The endemicity of the disease may not necessarily lead to high rate of fatal outcomes or treatment failures as reported in other parts of the world.

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Inhibitory effect of Lactobacillus acidophilus on Helicobacter hepaticus in vitro

Zhang

et al. 2013

World J Microbiol Biotechnol

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Helicobacter hepaticus and Helicobacter pylori both belong to Helicobacter species. Strains of Lactobacillus acidophilus, including L4 and L6, have shown significant inhibitory effects on H. pylori. Based on this phenomenon, we aim to investigate the inhibitory effect of L. acidophilus on H. hepaticus. Both standard and isolated H. hepaticus strains were grown under microaerophilic conditions at 37 °C in the presence of L. acidophilus supernatant, or lactic acid. The diameters of the inhibition zones were measured on the solid culture media. In liquid culture, the cell concentrations were measured and the urease activity was determined by phenol red staining. Sixteen strains of L. acidophilus isolated from human feces (named as L1-L16) showed anti-H. hepaticus effects. Two of them (L4 and L6) exhibited the most apparent effects on H. hepaticus inhibition. The L. acidophilus supernatant of L4 and L6 significantly increased the diameters of the inhibition zones compared with that of the lactic acid control (P < 0.05). The inhibitory role of L. acidophilus supernatant was independent of the pH value of solution (P > 0.05). Moreover, in liquid culture, L. acidophilus supernatant significantly reduced the cell growth rate and the urease activity of H. hepaticus cells in a time-dependent pattern (P < 0.05 compared with lactic acid control). No obvious difference was observed between the standard and isolated strain of H. hepaticus (P > 0.05). Our results indicate that L. acidophilus can decrease the viability and urease activity of H. hepaticus in vitro and this inhibition is independent of pH levels. This provides evidence for developing novel approaches for the prevention and treatment of H. hepaticus infection.

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Helicobacter pyloriinfection

Cited by 40 publications

References 159 publications

Helicobacter infection in hepatocellular carcinoma tissue

Helicobacter infection in hepatocellular carcinoma tissue

Helicobacter pylori Pathogenicity Associated cagA and vacA Genotypes Among Nigerian Dyspeptic Patients

Inhibitory effect of Lactobacillus acidophilus on Helicobacter hepaticus in vitro

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