<i>Helicobacter pylori</i> Antimicrobial Resistance and Risk Factors in Denmark 1998–2004: No Need for Concern?

Gjøde, P; Vinge, Ole Dybvad; Jensen, Søren; Krogfelt, Karen A.

doi:10.1111/j.1523-5378.2006.00402.x

Cited by 7 publications

(3 citation statements)

References 7 publications

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“…The present clarithromycin resistance (13.57%) is lower than early clarithromycin resistance prevalence (44.7) detected in Cameroon in 2006 [37], in America (29.3%) [39], in Japan (40.7%) [42], in Italy (36.7%) [45] and in Spain (49.2%) [46]. However, it is comparable to the resistance rate range of 11 to 15% reported in 2 studies performed in Iran [47, 48], in Bulgaria [40], in Denmark [49], in Italy [50], in Korea [43] and in Taiwan [44]. The differences between the resistance rates may reflect the variation in clarithromycin usage between countries.…”

Section: Discussionmentioning

confidence: 67%

Broad spectrum resistance in Helicobacter pylori isolated from gastric biopsies of patients with dyspepsia in Cameroon and efflux-mediated multiresistance detection in MDR isolates

et al. 2019

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BackgroundAntibiotic resistance is a leading cause of treatment failure in Helicobacter pylori infection. In Africa, there are very little data concerning the susceptibility of Helicobacter pylori isolates to antibiotics. The purpose of this study was to evaluate the resistance prevalence of Helicobacter pylori strains circulating in Cameroon, and to assess overexpression of efflux pump as a possible multi-drug resistance mechanisms.MethodsA total of 140 H. pylori isolates were recovered from gastric biopsies of dyspeptic patients in two reference hospitals in Cameroon and analyzed for their antimicrobial susceptibility to amoxicillin, co-amoxiclav, ampicillin, penicillin, imipenem, metronidazole, rifabutin, erythromycin, clarithromycin, azithromycin, levofloxacin, ciprofloxacin, norfloxacin, tetracycline, doxycycline and minocycline. Antibiotic sensitivity was tested by disk diffusion method. Phe-Arg-naphthylamide (PAßN) was used as efflux pump inhibitor. INT broth microdilution method in supplemented Brain Heart Infusion broth was used to determine the MIC of ampicillin, amoxicillin, metronidazole, erythromycin, clarithromycin and doxycycline in the absence and the presence of PAßN against 32 selected MDR isolates.ResultsOverall H. pylori resistance rate was 100% to ampicillin, penicillin and co-amoxiclav; 97.14% to amoxicillin, 97.85% to metronidazole, 47.85% to erythromycin, 13.57% to clarithromycin; 5, 2.86 and 0.71% to doxycycline, tetracycline and minocycline respectively. No resistance to azithromycin, rifabutin, imipenem, ciprofloxacin, norfloxacin and levofloxacin was detected among H. pylori isolates. Seventy percent (70%) of the tested isolates elicited a multiple drugs resistance pattern; 42.57% double, 15.71% triple and 5.71% quadruple drugs resistance. Metronidazole and amoxicillin were more concerned with double resistance pattern (86.76%). The spectrum of activity recorded with metronidazole, doxycycline, clarithromycin and erythromycin ranged from 0 to 100% in the absence to the presence of PAßN against the tested MDR isolates. An 8 to 128-fold increase in potency was also noticed with these antibiotics in the presence of PAßN.ConclusionWith regard to the high resistance rate to both amoxicillin and metronidazole, these drugs should be avoided as components of triple therapy in our milieu. In contrast, ciprofloxacin, norfloxacin, levofloxacin and tetracyclines could be used to achieve a better eradication rate and to reduce the risk of selection of H. pylori resistant strains.

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Section: Discussionmentioning

confidence: 67%

Broad spectrum resistance in Helicobacter pylori isolated from gastric biopsies of patients with dyspepsia in Cameroon and efflux-mediated multiresistance detection in MDR isolates

et al. 2019

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“…However, CLA are generally regarded as effective for treatment in Ireland. Increase in MTZ resistance was observed around the world as reported previously 22–25 and increasing CLA resistance was also seen, 26–28 although there is no increase in resistance in Denmark 29 . Different trends in drug susceptibility exist in various geographical regions and it is important to survey drug susceptibility continuously and to consider up‐to‐date treatment regimen in each region, including second‐line treatment in case of failure of first‐line treatment, for successful eradication.…”

Section: Discussionmentioning

confidence: 80%

Analysis of drug resistance and virulence‐factor genotype of Irish Helicobacter pylori strains: is there any relationship between resistance to metronidazole and cagA status?

Taneike¹,

Nami²,

OʼConnor

et al. 2009

Aliment Pharmacol Ther

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“…The prevalence of H. pylori resistance to antibiotics was tested in Denmark in 1997, 1998-2004, and 2013 [71][72][73]. Throughout the years, the resistance for clarithromycin has increased from 0% in 1997 to 53% in 2013, and likewise, the resistance for metronidazole increased from 20 to 74% [12][13][14].…”

Section: Prevalence Of H Pylori Resistance To Antibioticsmentioning

confidence: 99%

Gastric Microbiota and Resistance to Antibiotics

Stauning¹,

Nordestgaard²,

Frandsen³

et al. 2018

Helicobacter Pylori [Working Title]

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Studies on gastric microbiota find several bacterial families and species in the stomach using molecular-based techniques. When biopsies are cultured, there may be growth of bacteria, pure culture of Helicobacter pylori, or no growth. When looking at the histological sections of corresponding biopsies no bacteria may be seen, except curved rods (H. pylori) adherent to the gastric epithelial cells. In a number of biopsies, several different bacteria are cultured with or without H. pylori. The non-H. pylori bacteria cultured are like the normal oral flora and may be contamination of the samples during endoscopy. In histological sections, these bacteria are seen above the mucin layer and not adherent to the epithelial cells confirming that it is contamination of the samples and can thus not be regarded as gastric microbiota. Therefore, the susceptibility of H. pylori to antibiotics is independent of coexisting bacterial flora. A review of H. pylori susceptibility to antibiotics in untreated and previous treated patients will be given including meta-analyses of H. pylori susceptibility to metronidazole (MTZ), clarithromycin, and levofloxacin. These data indicate that these antibiotics become more doubtful to use for primary therapy and should be banned for secondary therapy without susceptibility testing.

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Helicobacter pylori Antimicrobial Resistance and Risk Factors in Denmark 1998–2004: No Need for Concern?

Cited by 7 publications

References 7 publications

Broad spectrum resistance in Helicobacter pylori isolated from gastric biopsies of patients with dyspepsia in Cameroon and efflux-mediated multiresistance detection in MDR isolates

Broad spectrum resistance in Helicobacter pylori isolated from gastric biopsies of patients with dyspepsia in Cameroon and efflux-mediated multiresistance detection in MDR isolates

Analysis of drug resistance and virulence‐factor genotype of Irish Helicobacter pylori strains: is there any relationship between resistance to metronidazole and cagA status?

Gastric Microbiota and Resistance to Antibiotics

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