<i>Geobacteraceae</i>
            Community Composition Is Related to Hydrochemistry and Biodegradation in an Iron-Reducing Aquifer Polluted by a Neighboring Landfill

Lin, Bor‐Shyh; Braster, Martin; Breukelen, Boris M. van; Verseveld, Henk W. van; Westerhoff, Hans V.; Röling, Wilfred F. M.

doi:10.1128/aem.71.10.5983-5991.2005

Cited by 53 publications

(56 citation statements)

References 33 publications

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“…Geobacter species were enriched in the Fe(III) reduction zone of a landfill leachate-contaminated aquifer in which Fe(III) reduction was considered to be involved in contaminant degradation (Roling et al, 2000(Roling et al, , 2001Lin et al, 2005;Mouser et al, 2005). Analysis of the 48 Geobacter sequences reported for this site indicated that in contrast to all of the other sites reported in this study sequences in Geobacter subsurface clade 2 were predominant.…”

Section: Geobacter Metallireducens (L07834)contrasting

confidence: 41%

Subsurface clade of Geobacteraceae that predominates in a diversity of Fe(III)-reducing subsurface environments

et al. 2007

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There are distinct differences in the physiology of Geobacter species available in pure culture. Therefore, to understand the ecology of Geobacter species in subsurface environments, it is important to know which species predominate. Clone libraries were assembled with 16S rRNA genes and transcripts amplified from three subsurface environments in which Geobacter species are known to be important members of the microbial community: (1) a uranium-contaminated aquifer located in Rifle, CO, USA undergoing in situ bioremediation; (2) an acetate-impacted aquifer that serves as an analog for the long-term acetate amendments proposed for in situ uranium bioremediation and (3) a petroleum-contaminated aquifer in which Geobacter species play a role in the oxidation of aromatic hydrocarbons coupled with the reduction of Fe(III). The majority of Geobacteraceae 16S rRNA sequences found in these environments clustered in a phylogenetically coherent subsurface clade, which also contains a number of Geobacter species isolated from subsurface environments. Concatamers constructed with 43 Geobacter genes amplified from these sites also clustered within this subsurface clade. 16S rRNA transcript and gene sequences in the sediments and groundwater at the Rifle site were highly similar, suggesting that sampling groundwater via monitoring wells can recover the most active Geobacter species. These results suggest that further study of Geobacter species in the subsurface clade is necessary to accurately model the behavior of Geobacter species during subsurface bioremediation of metal and organic contaminants.

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Section: Geobacter Metallireducens (L07834)contrasting

confidence: 41%

Subsurface clade of Geobacteraceae that predominates in a diversity of Fe(III)-reducing subsurface environments

et al. 2007

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“…Geobacter species have been shown to be important in the anaerobic degradation of petroleum components and landfill leachate contaminants in ground water [37][38][39][40][41]. The oxidation of the contaminant is linked to the reduction of Fe (III).…”

Section: Potential Applications For Microbial Fuel Cellsmentioning

confidence: 99%

Microbial Fuel Cells, A Current Review

2010

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Microbial fuel cells (MFCs) are devices that can use bacterial metabolism to produce an electrical current from a wide range organic substrates. Due to the promise of sustainable energy production from organic wastes, research has intensified in this field in the last few years. While holding great promise only a few marine sediment MFCs have been used practically, providing current for low power devices. To further improve MFC technology an understanding of the limitations and microbiology of these systems is required. Some researchers are uncovering that the greatest value of MFC technology may not be the production of electricity but the ability of electrode associated microbes to degrade wastes and toxic chemicals. We conclude that for further development of MFC applications, a greater focus on understanding the microbial processes in MFC systems is required.

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“…Geobacter species are important agents in the bioremediation of organic contaminants (Lovley et al, 1989;Rooney-Varga et al, 1999;Lin et al, 2005), as well as uranium (Anderson et al, 2003;North et al, 2004;Vrionis et al, 2005) and vanadium (Ortiz-Bernad et al, 2004b). Analysis of gene transcript abundance within the subsurface Geobacter community has been successful in diagnosing rates of metabolism in Geobacter species as well as nutrient limitations and stress responses (Holmes et al, 2004b(Holmes et al, , 2005O'Neil et al, 2008;Mouser et al, 2009a).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

et al. 2009

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Nutrient limitation is an environmental stress that may reduce the effectiveness of bioremediation strategies, especially when the contaminants are organic compounds or when organic compounds are added to promote microbial activities such as metal reduction. Genes indicative of phosphatelimitation were identified by microarray analysis of chemostat cultures of Geobacter sulfureducens. This analysis revealed that genes in the pst-pho operon, which is associated with a high-affinity phosphate uptake system in other microorganisms, had significantly higher transcript abundance under phosphate-limiting conditions, with the genes pstB and phoU upregulated the most. Quantitative PCR analysis of pstB and phoU transcript levels in G. sulfurreducens grown in chemostats demonstrated that the expression of these genes increased when phosphate was removed from the culture medium. Transcripts of pstB and phoU within the subsurface Geobacter species predominating during an in situ uranium-bioremediation field experiment were more abundant than in chemostat cultures of G. sulfurreducens that were not limited for phosphate. Addition of phosphate to incubations of subsurface sediments did not stimulate dissimilatory metal reduction. The added phosphate was rapidly adsorbed onto the sediments. The results demonstrate that Geobacter species can effectively reduce U(VI) even when experiencing suboptimal phosphate concentrations and that increasing phosphate availability with phosphate additions is difficult to achieve because of the high reactivity of this compound. This transcript-based approach developed for diagnosing phosphate limitation should be applicable to assessing the potential need for additional phosphate in other bioremediation processes.

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Geobacteraceae Community Composition Is Related to Hydrochemistry and Biodegradation in an Iron-Reducing Aquifer Polluted by a Neighboring Landfill

Cited by 53 publications

References 33 publications

Subsurface clade of Geobacteraceae that predominates in a diversity of Fe(III)-reducing subsurface environments

Subsurface clade of Geobacteraceae that predominates in a diversity of Fe(III)-reducing subsurface environments

Microbial Fuel Cells, A Current Review

Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

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