<i>Flavobacterium johnsoniae</i>
            SprA Is a Cell Surface Protein Involved in Gliding Motility

Nelson, Shawn S.; Glocka, Padden P.; Agarwal, Sakshi; Grimm, David P.; McBride, Mark J.

doi:10.1128/jb.00892-07

Cited by 48 publications

(56 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Five of the genes described above, porK, porL, porM, sov, and porW, are similar to F. johnsoniae gliding motility genes gldK, gldL, gldM, sprA, and sprE, respectively (17,18). In F. johnsoniae, gldN, which is also involved in gliding motility, is located immediately downstream of gldK, gldL, and gldM.…”

Section: Identification Of Genes Involved In Gingipain Secretion Bymentioning

confidence: 92%

A protein secretion system linked to bacteroidete gliding motility and pathogenesis

Satô

Naito²,

Yukitake³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

312

648

View full text Add to dashboard Cite

Porphyromonas gingivalis secretes strong proteases called gingipains that are implicated in periodontal pathogenesis. Protein secretion systems common to other Gram-negative bacteria are lacking in P. gingivalis, but several proteins, including PorT, have been linked to gingipain secretion. Comparative genome analysis and genetic experiments revealed 11 additional proteins involved in gingipain secretion. Six of these (PorK, PorL, PorM, PorN, PorW, and Sov) were similar in sequence to Flavobacterium johnsoniae gliding motility proteins, and two others (PorX and PorY) were putative two-component system regulatory proteins. Real-time RT-PCR analysis revealed that porK, porL, porM, porN, porP, porT, and sov were down-regulated in P. gingivalis porX and porY mutants. Disruption of the F. johnsoniae porT ortholog resulted in defects in motility, chitinase secretion, and translocation of a gliding motility protein, SprB adhesin, to the cell surface, providing a link between a unique protein translocation system and a motility apparatus in members of the Bacteroidetes phylum.gingipain | Porphyromonas gingivalis | Flavobacterium | chitinase

show abstract

Section: Identification Of Genes Involved In Gingipain Secretion Bymentioning

confidence: 92%

A protein secretion system linked to bacteroidete gliding motility and pathogenesis

Satô

Naito²,

Yukitake³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

312

648

View full text Add to dashboard Cite

show abstract

“…The porT orthologs have not been identified outside the Bacteroidetes phylum. They have been identified in the genomes of Cytophaga hutchinsonii ATCC 33406 and Flavobacterium johnsoniae but not in those of colon bacteria, Bacteroides fragilis and Bacteroides thetaiotaomicron, which are more closely related to P. gingivalis 21,22 . In addition to porT, 55 homologous genes were detected in both P. gingivalis and C. hutchinsonii but not in B. thetaiotaomicron Fig.…”

Section: P Gingivalis Genes Involved In Gingipain Secretionmentioning

confidence: 99%

“…Five of these genes, porK, porL, porM, sov, and porW, are homologous to the F. johnsoniae gliding motility genes, gldK, gldL, gldM, sprA, and sprE, respectively 21,22 . Sov PGN_0832 was reported to be involved in the secretion of gingipains and the sov mutant showed decreased activities of Rgp and Kgp in both whole cells and supernatants 26 .…”

Section: P Gingivalis Genes Involved In Gingipain Secretionmentioning

confidence: 99%

“…The mechanism of this gliding is unknown but it is not connected with flagellar motility, type pilus mediated twitching motility, or mycoplasma gliding motility 27 . Previous studies have revealed that 16 genes gldA, gldB, gldD, gldF, gldH, gldI, gldJ, gldK, gldL, gldM, gldN, sprA, sprB, sprC, sprD, and sprE are involved in gliding motility and chitinase activity in F. johnsoniae 21,22,27,28 . The porT ortholog sprT defective mutant showed no gliding motility on 1.5 solid agar Fig.…”

Section: F Johnsoniae Sprt Mutants Are Defective In Gliding Motilitymentioning

confidence: 99%

“…Gliding motility defective mutants showed very low chitinase activity 21 . To examine whether this was the result of failed chitinase secretion, the chitinase activities of sprT mutant, sprT complemented, and wild type strains were determined with three sub- found in sprT complemented and wild type strains but not in the sprT mutant by using SDS PAGE and a peptide mass fingerprint mass spectrometer.…”

Section: F Johnsoniae Sprt Mutant Is Defective In Extracellular Chitmentioning

confidence: 99%

See 2 more Smart Citations

Por Secretion System of Porphyromonas gingivalis

Satô¹

2011

J. Oral Biosci.

View full text Add to dashboard Cite

Flavobacterium

Bernardet

Bowman

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Fla.vo.bac.te'ri.um. L. adj. flavus yellow; L. neut. n. bacterium a rod or staff and, in biology, a bacterium (so called because the first ones observed were rod‐shaped); N.L. neut. n. Flavobacterium a yellow bacterium. Bacteroidetes / Flavobacteriia / Flavobacteriales / Flavobacteriaceae / Flavobacterium Straight or slightly curved, single rods with rounded or slightly tapered ends typically about 0.3–0.5 µm in diameter and variable in length, often 2–5 µm; shorter (1 µm) or filamentous (10–40 µm) cells and pleomorphism also occur. The longer rods are flexible. Do not form endospores. Several species produce spherical degenerative forms (spheroplasts) in stationary growth phase. Nonmotile or motile by gliding. Flagella have not been reported. Colonies are pale to bright yellow due to the production of nondiffusible, nonfluorescent carotenoid or flexirubin types of pigments or both . Gram‐stain‐negative. Chemoorganotrophic. Most species are obligately aerobic, having a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor. A few species may also grow weakly under microaerobic to anaerobic conditions. About half of the species are able to reduce nitrate to nitrite, but only one species is able to carry out complete denitrification . The optimum growth temperature range is 20–30°C for most temperate species and 15–20°C for most cold‐living species. Most species grow readily on nutrient and tryptic soy agars; no growth factors are required. Most species also grow on media containing up to 2–4% NaCl. Usually positive for catalase and oxidase . About half of the species are able to oxidize carbohydrates. Esculin and starch are hydrolyzed by most species, but agar and carboxymethylcellulose are hydrolyzed by only a few species. Strong proteolytic activity occurs. Menaquinone MK‐6 is the only or predominant respiratory quinone. Predominant cellular fatty acids are C 15:0 , C 15:0 iso, C 15:0 iso 3‐OH, C 15:0 anteiso, C 15:1 ω6 c , C 15:1 iso G , C 16:0 iso 3‐OH, and C 17:0 iso 3‐OH, as well as C 15:0 iso 2‐OH and/or C 16:1 ω7 c and/or C 16:1 ω7 t . Sphingophospholipids are absent in all tested species. Homospermidine is the major polyamine in all tested species. Occur in soil and in freshwater, marine, or saline environments in warm, temperate, or polar locations. Three species are pathogenic for freshwater fish and three others have occasionally been isolated from diseased freshwater fish. DNA G + C content ( mol %): 30–41. Type species : Flavobacterium aquatile (Frankland and Frankland 1889) Bergey, Harrison, Breed, Hammer and Huntoon 1923, 100 ( Bacillus aquatilis Frankland and Frankland 1889, 381) (not Flavobacterium antarcticum as erroneously printed in Bernardet and Bowman, 2006).

show abstract

Flavobacterium johnsoniae SprA Is a Cell Surface Protein Involved in Gliding Motility

Cited by 48 publications

References 34 publications

A protein secretion system linked to bacteroidete gliding motility and pathogenesis

A protein secretion system linked to bacteroidete gliding motility and pathogenesis

Por Secretion System of Porphyromonas gingivalis

Flavobacterium

Contact Info

Product

Resources

About