Ex vivo evaluation of various instrumentation techniques and irrigants in reducing E. faecalis within root canals

Abstract

No significant differences in terms of reduction in microbial counts were observed between single-file techniques (SAF and Reciproc) and serial Ni-Ti instrumentation technique (ProTaper) in combination with irrigants.

Evaluation of Microbial Reduction in Root Canals Instrumented with Reciprocating and Rotary Systems

“…Koncept korištenja jedne iglice u instrumentaciji kanala zanimljiv je zato što znatno smanjuje radno vrijeme pa je ugodniji i pacijentu i kliničaru. Unatoč tomu postoji problem s tim konceptom, a riječ je o mogućnosti čišćenja korijenskog kanala upravo zbog kraće instrumentacije, manje količine otopine za ispiranje i manje prodornosti otopine, što sve zajedno smanjuje mogućnost uklanjanja mikroorganizama iz korijenskih kanala (10,11).…”

“…Jedini problem, i pitanje koje se nameće, jest može li jedna iglica instrumentirati i dezinficirati korijenski kanal zbog skraćenoga vremena preparacije, manje količine antimikrobne otopine i kraće izloženosti otopini (10,11).…”

“…Iako se nekoliko dosadašnjih istraživanja fokusiralo na učinkovitost čišćenja (12), apikalno izbacivanje otpada (13) te na redukciju endotoksina (14) jednoigličnog sistema, u nekoliko studija (10,11,15,16) analizirana je mogućnost smanjivanja broja mikroorganizama u kontaktu s antimikrobnom otopinom za ispiranje.…”

“…A concern has been presented, however, regarding the ability of a single-file instrumentation system to disinfect the root canal, due to the shortening of preparation time of the canal, together with the lesser amount of antimicrobial agent and shorter contact time (10,11).…”

“…Thus, although several previous studies have evaluated the cleaning efficacy (12), the apical extrusion of debris (13), and the reduction of endotoxins (14) of single-file systems, only few studies (10,11,15,16) have analyzed the ability to reduce intracanal microbial agents when associated with antimicrobial irrigating solutions.…”