<i>Enterococcus faecalis</i> with capsule polysaccharides type 2 and biofilm‐forming capacity in Indonesians requiring endodontic treatment

Bachtiar, Endang W.; Dewiyani, Sari; Akbar, Siti M. Surono

doi:10.1111/jicd.12143

Cited by 19 publications

(24 citation statements)

References 42 publications

(63 reference statements)

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Section: Polymorphism Of Polysaccharide Capsulementioning

confidence: 99%

“…22 In their studies Bachtiar and coworkers reported that E. faecalis species had Cps-2 were more prevalent in primary and secondary endodontic patients. 22 This difference might be caused by individual variation or unique environment triggering endodontic infections among populations. 41,42 Similar to this study, Bachtiar and coworkers did not explain whether the E. faecalis had Cps 2 reported were isolates with positive or negative Cps phenotypes.…”

Section: Polymorphism Of Polysaccharide Capsulementioning

confidence: 99%

“…20 This study focused on Cps operons of E. faecalis in primary endodontic patients with or without periapical lesions, different from other researchers that did not address periapical lesions in the primary endodontic patients investigated. 22,23 gelE and ace nucleotide sequences The variation observed in the activity of gelatinase and the level of mRNA ace expression of E. faecalis isolates collected from primary endodontic patients with or without periapical lesions might relate to the polymorphism of gelE and ace, the encoding genes. To address this assumption, coding regions for both gelE and ace preliminary were separately amplified by PCR, sequenced and then analyzed using web-based 3730xl DNA Analyze software programs.…”

Section: 23mentioning

confidence: 99%

“…[17][18][19][20] Additionally, the avail-able data explaining the relationship between the polymorphism of E. faecalis Cps and root canal infections is still controversial. [21][22][23] Ace is the solely virulence factor of E. faecalis that has been known its role in facilitating the adherence of this bacterium in the collagen of human dentin. 20 The data suggest that, except for that of Ace, the actual roles of virulence factors produced by E. faecalis in the pathogenesis of enterococcal infections in the root canal or dental tubuli are not clearly understood or experimentally proved.…”

Section: Introductionmentioning

confidence: 99%

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Phenotype and Genotype of Enterococcus faecalis Isolated form Root Canal and Saliva of Primary Endodontic Patients

et al. 2016

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Objective: To investigate the phenotype and genotype of E. faecalis isolated from the root canal and saliva of primary endodontic patients with periapical lesions. Methods: Eighteen adult male and female individuals suffering from primary endodontic infection, either with or without periapical lesions, were involved in this study. Root canal scraping and saliva were collected from each subject and used for bacterial quantitation using a real-time polymerase chain reaction (RT-PCR). Enterococci were isolated using ChromAgar medium and then identified using both biochemical (Gram staining and catalase tests) and molecular biology (conventional PCR) methods. Gelatinase activity, polysaccharide capsul profile and mRNA ace expression level were determined using microbiological, biochemical and molecular biology approach, respectively. Genotype of E. faecalis was determined based on nucleotide sequence of ace and gelE genes analyzed using web-based 3730xl DNA Analyze software. Results:The results showed that except for its proportion, no significant difference was found in phenotypes (gelatinase activity and mRNA ace expression levels) and genotypes (polymorphism of Cps operon and variation of ace and gelE nucleotide sequences) of E. faecalis isolated from the root canal and saliva of primary endodontic patients had or had no periapical lesions. Conclusion: It can be concluded that E. faecalis proportion had a role in the occurrence of periapical lesions in the primary endodontic patients, but not gelatinase activity, mRNA ace expression level, Cps operon polymorphism or ace and gelE nucleotide sequence variations.

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Section: Polymorphism Of Polysaccharide Capsulementioning

confidence: 99%

Section: Polymorphism Of Polysaccharide Capsulementioning

confidence: 99%

Section: 23mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotype and Genotype of Enterococcus faecalis Isolated form Root Canal and Saliva of Primary Endodontic Patients

et al. 2016

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“…The extraction of violet crystals from microorganisms was effected by adding 100 μl of 98% ethanol prior to agitating the solution for 5 min at 300 rpm. 15 Optical density (OD) of serial duplo biofilm was then measured with an Elisa Reader (Bio-Rad Laboratories Inc., CA, USA) using a wavelength of 590 nm.…”

mentioning

confidence: 99%

The efficacy of sarang semut extract (Myrmecodia pendens Merr & Perry) in inhibiting Porphyromonas gingivalis biofilm formation

Alibasyah¹,

Purba²,

Setiabudiawan³

et al. 2017

Dent. J. (Maj. Ked. Gigi)

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Inhibition of Candida albicans biofilm development by unencapsulated Enterococcus faecalis cps2

Bachtiar

Dewiyani

Akbar

2016

Journal of Dental Sciences

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Background/purpose In the oral environment, Candida albicans interacts with many bacteria, including Enterococcus faecalis . We investigated the susceptibility of C. albicans biofilm development to the presence of unencapsulated E. faecalis cps2 in comparison with reference strains ( E. faecalis ATCC 29212) or their respective spent medium (collected at 6 hours). Material and methods Crystal violet stain was used to measure the total biofilm mass, whereas quantitative real-time polymerase chain reaction was used to analyze the change in expression of the mRNA of hypha morphology (ALS1 and ALS3) and biofilm maturation (EFB1). Results At the intermediate stage, C. albicans resisted the presence of each E. faecalis strain tested and their spent medium. However, at the maturation stage, the unencapsulated strain was stronger in reducing C. albicans biofilms than the reference strain (P < 0.05). At this maturation stage, the transcription levels of each gene tested decreased in the presence of either E. faecalis strains or their respective spent medium. The unencapsulated strain was more pronounced in reducing ALS1/ALS3 expression, whereas the respective spent medium had a similar capability to restrict the expression of EFB1. Conclusion This study showed, the unencapsulated strain is more effective in inhibiting C. albicans biofilm development compared with the reference strains. In contrast, the secreted molecules produced by each strain tested are necessary in controlling the growths of C. albicans biofilm.

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Enterococcus faecalis with capsule polysaccharides type 2 and biofilm‐forming capacity in Indonesians requiring endodontic treatment

Cited by 19 publications

References 42 publications

Phenotype and Genotype of Enterococcus faecalis Isolated form Root Canal and Saliva of Primary Endodontic Patients

Phenotype and Genotype of Enterococcus faecalis Isolated form Root Canal and Saliva of Primary Endodontic Patients

The efficacy of sarang semut extract (Myrmecodia pendens Merr & Perry) in inhibiting Porphyromonas gingivalis biofilm formation

Inhibition of Candida albicans biofilm development by unencapsulated Enterococcus faecalis cps2

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