<i>Enterobacter gergoviae</i>
 membrane modifications are involved in the adaptive response to preservatives used in cosmetic industry

Périamé, Marina; Pagès, Jean‐Marie; Davin-Régli, Anne

doi:10.1111/jam.12676

Cited by 8 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An adaptative mechanism probably occurred in the isolate, enabling it to overcome growth inhibition by the protective ingredients we tested. A similar adaptation was already reported for P. gergoviae inoculated in various cosmetics containing frequently used preservatives (Périamé et al, 2014, 2015).…”

Section: Discussionsupporting

confidence: 71%

Real-time qPCR to evaluate bacterial contamination of cosmetic cream and the efficiency of protective ingredients

Bermond

Cherrad²,

Trainoy

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims The absence of objectionable micro‐organisms in cosmetics and the efficiency of preservatives are still mainly assessed by time‐consuming cultivation‐based methods. We explored the applicability of real‐time quantitative polymerase chain reaction (qPCR) and reported on the behaviour of different bacteria in artificially contaminated creams. Methods and results Real‐time qPCR on DNA from Burkholderia cepacia, Pluribacter gergoviae, Pseudomonas aeruginosa and Sphingomonas paucimobilis identified specific primer pairs that amplify accurately and efficiently two strains/isolates of each species. Using DNeasy mericon Food Kit, we detected bacterial growth in an inoculated cosmetic cream and persistency of DNA from heat‐inactivated bacteria. We were also able to monitor the growth inhibitory effect of caprylyl glycol and EDTA, also showing how different bacterial species interact depending on the presence/absence of these ingredients. Finally, creams supplemented with the protective cosmetic ingredients revealed the various behaviour of five strains/isolates from P. aeruginosa. Conclusions Successfully extracting bacterial DNA from artificially contaminated cosmetic creams, we could perform real‐time qPCR to identify and follow the growth of various strains of 4 bacteria species under different conditions. Significance and impact of the study Real‐time qPCR appears as a promising method to detect bacterial contamination in cosmetic creams and/or to monitor growth inhibition by ingredients.

show abstract

Section: Discussionsupporting

confidence: 71%

Real-time qPCR to evaluate bacterial contamination of cosmetic cream and the efficiency of protective ingredients

Bermond

Cherrad²,

Trainoy

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Recently, however, ESBL type SHV and carbapenemase type (IMP or KPC) producers were described in this species (113). With regard to biocides, due to membrane modifications, esterase production, and the modulation of enzymes involved in oxidative detoxification, this species has a natural resistance to the parabens, triclosan, and methylisothiazolinonechloromethylisothiazolinone (MIT-CMIT), which are preservatives used in this type of product (114,115). Such results explain the ability of this species to contaminate cosmetics from a source probably of unknown plant origin (116,117).…”

Section: Enterobacter Gergoviaementioning

confidence: 99%

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

2019

Self Cite

View full text Add to dashboard Cite

SUMMARY The genus Enterobacter is a member of the ESKAPE group, which contains the major resistant bacterial pathogens. First described in 1960, this group member has proven to be more complex as a result of the exponential evolution of phenotypic and genotypic methods. Today, 22 species belong to the Enterobacter genus. These species are described in the environment and have been reported as opportunistic pathogens in plants, animals, and humans. The pathogenicity/virulence of this bacterium remains rather unclear due to the limited amount of work performed to date in this field. In contrast, its resistance against antibacterial agents has been extensively studied. In the face of antibiotic treatment, it is able to manage different mechanisms of resistance via various local and global regulator genes and the modulation of the expression of different proteins, including enzymes (β-lactamases, etc.) or membrane transporters, such as porins and efflux pumps. During various hospital outbreaks, the Enterobacter aerogenes and E. cloacae complex exhibited a multidrug-resistant phenotype, which has stimulated questions about the role of cascade regulation in the emergence of these well-adapted clones.

show abstract

“…Besides a case of two patients with recurrent corneal erosion, syndrome connected with the usage of lenses colonized by E. gergoviae was reported . However, the problem of cosmetics’ contamination by E. gergoviae is widely described in the literature in the context of the adaptation of this species to preservatives commonly used in the cosmetics industry …”

Section: Discussionmentioning

confidence: 99%

“…38 However, the problem of cosmetics' contamination by E. gergoviae is widely described in the literature in the context of the adaptation of this species to preservatives commonly used in the cosmetics industry. 39,40 One unanticipated finding was that most of the microbiologically contaminated cosmetics registered in the Rapex system originated from European countries. This finding is contrary to our previous research on skin-lightening products, characterized mainly with chemical risks, registered in the same system, where most of the products originated from non-European countries.…”

Section: Main Findings Of the Studymentioning

confidence: 99%

Microbiological contamination of cosmetic products – observations from Europe, 2005–2018

Michałek

John

Santos

2019

Acad Dermatol Venereol

View full text Add to dashboard Cite

Background Although cosmetic products are not expected to be fully aseptic, their potential microbiological contamination might be dangerous, especially for immunocompromised patients. The extent of such contamination of cosmetics in Europe is poorly studied. Objectives The objective of this study was to explore whether microbiologically contaminated cosmetic products are available for sale in Europe and what type of contamination was reported. Methods We searched the European Union Rapid Information System for dangerous non‐food products (Rapex), a database of non‐compliant products among 31 European countries, to identify microbiologically contaminated cosmetics reported between 2005 and 2018, and present a detailed summary of these notifications. Results In the years 2005–2018, 104 reports on microbiologically contaminated cosmetics were identified. Twenty of them were products for children. The majority of the products (65.38%) were produced in Rapex member states. In most cases, contamination was caused by Gram‐negative bacteria (59.62%), mostly Pseudomonas spp. (35.58%) and Enterobacter spp. (11.54%). Conclusions Gram‐negative rod‐shaped bacteria are the most common microbiota contaminating cosmetic products in Europe. Most of the reported microbiologically contaminated cosmetics originated from European countries.

show abstract

Enterobacter gergoviae membrane modifications are involved in the adaptive response to preservatives used in cosmetic industry

Abstract: No cross-resistance could be observed with antibiotics when MICs to preservatives were increased; however, a decrease in the disinfectants bactericidal effects was confirmed in preservative-tolerant strains. This will impact industry disinfection strategies treatment against bacteria.

Cited by 8 publications

References 51 publications

Real-time qPCR to evaluate bacterial contamination of cosmetic cream and the efficiency of protective ingredients

Real-time qPCR to evaluate bacterial contamination of cosmetic cream and the efficiency of protective ingredients

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

Microbiological contamination of cosmetic products – observations from Europe, 2005–2018

Contact Info

Product

Resources

About