<i>Echinococcus granulosus:</i>diagnosis of hydatid disease in man

Dottorini, S; Sparvoli, M; Bellucci, C.; Magnini, M.

doi:10.1080/00034983.1985.11811887

Cited by 14 publications

(8 citation statements)

References 11 publications

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“…Certain high-molecular-weight fractions, identi® ed in the scolices of sheep cysts, protected mice against experimental secondary infection with the cestode (De-Rosa et al, 1977;Pauluzzi et al, 1978). Dottorini et al (1985) also found that use of a crude extract of the scolices from sheep cysts gave good sensitivity in IHA. However, in contrast to the present observations, Tassi et al (1981) and Dottorini et al (1985) all achieved higher sensitivities when they used CFA than when they used a preparation of scolices, in several types of serological test.…”

Section: Discussionmentioning

confidence: 90%

Fertility of the cysts of Echinococcus granulosus in domestic herbivores from Benghazi, Libya, and the reactivity of antigens produced from them

Khan

El-Buni²,

Ali³

2001

Annals of Tropical Medicine And Parasitology

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Unilocular cysts produced by Echinococcus granulosus were recovered from 110 domestic herbivores (60 sheep, 25 cattle, 20 goats and five camels) slaughtered in Benghazi. The proportion of the cysts from the sheep found to be fertile (75%) was higher than that of the cysts from the goats (55%), camels (40%), or cattle (0%). When tested in indirect haemagglutination assays (IHA) with eight sera from human cases of cystic echinococcosis, the fluid from the cattle cysts never gave a positive reaction. Antigens in the fluids collected from sheep or goat cysts did react with the sera, with antigens from each of the two sources giving similar titres with each serum. However, crude somatic antigens (prepared from protoscolices and brood capsules collected from sheep cysts) appeared to be more sensitive for the immunodiagnosis of human cystic echinococcosis than the cyst-fluid antigens.

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Section: Discussionmentioning

confidence: 90%

Fertility of the cysts of Echinococcus granulosus in domestic herbivores from Benghazi, Libya, and the reactivity of antigens produced from them

Khan

El-Buni²,

Ali³

2001

Annals of Tropical Medicine And Parasitology

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“…Protoscoleces antigen was elaborated in accord with Dottorini et al (26). Protein was assessed in accord with Schacterle and Pollack (27).…”

Section: Elaboration Of Antigen and Assessment Of Foot Pad Responsementioning

confidence: 99%

Immunity response of mice infected with laser irradiated protoscoleces of Echinococcus granulosus

Zaki¹,

Ali²

2021

J.Edu.Sci.

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The present study investigated the effect of the Neodymium-Doped Yttrium Aluminum Garnet (Nd: Y3AL5G12) Laser eradiation 1000 mw, against infection with secondary hydatid disease in BALB/c mice by inoculating the animals with protoscoleces of Echinococcus granulosus exposed to laser irradiation, for various periods 40,60,120 and 240 minutes, with viability of 70%,57%,50% and 40%, respectively, contrasted to the control set (mice inoculated with unexposed protoscoleces) along three months, depending on the evaluation of the total count of WBCs and assessment of acquired cell-mediated immunity, demonstrated by delayed type hypersensitivity test (DTH). The results showed considerable increase (p<0.01) in the total WBCs counting, in mice injected with irradiated protoscoleces, up to 13962 cell/cm 3 , in comparison to the control group 5420 cell/cm 3 , after 120 minutes, two months post infection. Moreover, there was considerable excess (p<0.01) in the foot pad thickness in treated mice, 2.94 mm after 120 minute (24hour post antigen injection), two months post infection, 2.86mm after 120 minutes (24 hours post antigen injection), 1 month post infection and 2.64 mm, 120 minutes (24 hours post antigen injection), three months post infection, compared with the control group 1.18mm, 1.80mm and 1.72mm, respectively.Results revealed that irradiation of Echinococcus granulosus protoscoleces with laser had a significant influence on the production of WBCs and stimulation of delayed-type hypersensitivity reaction in mice.

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“…PSC antigen was prepared according to Dottorini et al, [16]. Protein was estimated according to Lowry et.…”

Section: Protoscoleces Antigenmentioning

confidence: 99%

The Effect of Protoscoleces Treated With Direct Electrical Current on the Cellular Immune Response in Mice Infected With Secondary Hydatid Disease

Yaseen¹

2010

J.Edu.Sci.

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An obvious significant increase (P < 0.01) occurred in rate of foot pad thickness 1.83 mm. in immunized group with PSC treated with direct electrical current with potential 5.77mA/cm 2 for 2 min. whereas, mice immunized with PSC treated with direct current potential 11.82 mA/cm 2 for 2min. too, led to significant increases (P <0.01) in rate of mitotic activity of spleen and bone marrow cells, 61.8%, 70.1% for each, respectively.

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Echinococcus granulosus:diagnosis of hydatid disease in man

Cited by 14 publications

References 11 publications

Fertility of the cysts of Echinococcus granulosus in domestic herbivores from Benghazi, Libya, and the reactivity of antigens produced from them

Fertility of the cysts of Echinococcus granulosus in domestic herbivores from Benghazi, Libya, and the reactivity of antigens produced from them

Immunity response of mice infected with laser irradiated protoscoleces of Echinococcus granulosus

The Effect of Protoscoleces Treated With Direct Electrical Current on the Cellular Immune Response in Mice Infected With Secondary Hydatid Disease

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