<i>Drosophila</i> Boi limits Hedgehog levels to suppress follicle stem cell proliferation

Hartman, Tiffiney R.; Zinshteyn, Daniel; Schofield, Heather; Nicolas, Émmanuelle; Okada, Ami; O’Reilly, Alana M.

doi:10.1083/jcb.201007142

Cited by 70 publications

(120 citation statements)

References 53 publications

(93 reference statements)

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“…Three independent Gal4 insertions into the hh locus (hh GMR28E03 , hh GMR29D09 , and hh GMR28B04 ) gave very specific expression in terminal filament and cap cells (Eliazer et al 2011) but no detectable expression in IGS cells, FSCs, or follicle cells in the germarium ( Figure 1B). This is consistent with previous data demonstrating that Hh protein is present and functional in these cells (Hartman et al 2010(Hartman et al , 2013Sahai-Hernandez and Nystul 2013) and distinguishes these lines from a hh-lacZ reporter gene that also is expressed in escort cells (Forbes et al 1996a). As expected, insertions in other components of the Hh signaling pathway such as boi-Gal4 NP4065 gave similar patterns of expression ( Figure 1B).…”

Section: Resultssupporting

confidence: 82%

“…In addition, weak expression in developing follicle cells was observed ( Figure 1B) (Cabrera et al 2002;Hartman et al 2010). 109-53 Gal4 exhibited a more specific expression pattern in terminal filament and cap cells, as well as stalk cells, with no detectable expression in follicle cells ( Figure 1B) (Hartman et al 2013).…”

Section: Resultsmentioning

confidence: 99%

“…In most cases, expression patterns have been mapped in developing embryos and selected tissues, but patterns of expression within subsets of cells in the germarium have not been clearly defined. To identify Gal4 lines that are expressed in somatic cells of the germarium, including (1) terminal filament and cap cells, (2) IGS cells, (3) FSCs, and (4) follicle cells ( Figure 1A), we used a candidate approach in which lines that contain Gal4 inserted into known genes that function in a particular somatic cell population within the ovary [e.g., bric-à-brac 1 (bab1) and hedgehog (hh) function in terminal filament and cap cells (Cabrera et al 2002;Hartman et al 2010Hartman et al , 2013Sahai-Hernandez and Nystul 2013)] were screened as well as lines in which previous studies had indicated general ovarian expression (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…The 109-30-Gal4 driver is expressed in FSCs and all FSC daughter cells up to stage 3 ( Figure 5A) (Hartman et al 2010). After stage 3, 109-30 Gal4 promotes UAS-GFP expression in stalk cells only ( Figure 5B) (Hartman et al 2010).…”

Section: -30-gal4-labeled Fscs Exhibit Novel Cellular Projectionsmentioning

confidence: 99%

“…Nutrient restriction was performed for 1, 2, or 3 days. Fly ovaries were prepared as described by Hartman et al (2010). Ovaries were dissected, fixed in 4% paraformaldehyde, and immunostained with mouse anti-Fas3 (1:25; DSHB) (Patel et al 1987) and rabbit anti-phospho-histone H3 (1:1000; Millipore, Bedford, MA).…”

Section: Nutrient Restrictionmentioning

confidence: 99%

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Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation

et al. 2015

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In many tissues, the presence of stem cells is inferred by the capacity of the tissue to maintain homeostasis and undergo repair after injury. Isolation of self-renewing cells with the ability to generate the full array of cells within a given tissue strongly supports this idea, but the identification and genetic manipulation of individual stem cells within their niche remain a challenge. Here we present novel methods for marking and genetically altering epithelial follicle stem cells (FSCs) within the Drosophila ovary. Using these new tools, we define a sequential multistep process that comprises transitioning of FSCs from quiescence to proliferation. We further demonstrate that integrins are cell-autonomously required within FSCs to provide directional signals that are necessary at each step of this process. These methods may be used to define precise roles for specific genes in the sequential events that occur during FSC division after a period of quiescence.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: -30-gal4-labeled Fscs Exhibit Novel Cellular Projectionsmentioning

confidence: 99%

Section: Nutrient Restrictionmentioning

confidence: 99%

See 3 more Smart Citations

Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation

et al. 2015

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Genetic Interaction Screens Identify a Role for Hedgehog Signaling in Drosophila Border Cell Migration

Geisbrecht

Sawant

et al. 2013

Developmental Dynamics

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Background Cell motility is essential for embryonic development and physiological processes such as the immune response, but also contributes to pathological conditions such as tumor progression and inflammation. However, our understanding of the mechanisms underlying migratory processes is incomplete. Drosophila border cells provide a powerful genetic model to identify the roles of genes that contribute to cell migration. Results Members of the Hedgehog signaling pathway were uncovered in two independent screens for interactions with the small GTPase Rac and the polarity protein Par-1 in border cell migration. Consistent with a role in migration, multiple Hh signaling components were enriched in the migratory border cells. Interference with Hh signaling by several different methods resulted in incomplete cell migration. Moreover, the polarized distribution of E-Cadherin and a marker of tyrosine kinase activity were altered when Hh signaling was disrupted. Conservation of Hh-Rac and Hh-Par-1 signaling was illustrated in the wing, in which Hh-dependent phenotypes were enhanced by loss of Rac or par-1. Conclusions We identified a pathway by which Hh signaling connects to Rac and Par-1 in cell migration. These results further highlight the importance of modifier screens in the identification of new genes that function in developmental pathways.

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Drosophila models of epithelial stem cells and their niches

Sahai‐Hernandez

Castanieto

Nystul

2012

WIREs Developmental Biology

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Epithelial stem cells are regulated through a complex interplay of signals from diffusible ligands, cellular interactions, and attachment to the extracellular matrix. The development of Drosophila models of epithelial stem cells and their associated niche has made it possible to dissect the contribution of each of these factors in vivo, during both basal homeostasis and in response to acute damage such as infection. Studies of Drosophila epithelial stem cells have also provided insight into the mechanisms by which a healthy population of stem cells are maintained throughout adulthood by demonstrating, for example, that stem cells have a finite lifespan and may be displaced by replacement cells competing for niche occupancy. Here, we summarize the literature on each of the known Drosophila epithelial stem cells, with a focus on the two most well characterized types, the follicle stem cells (FSCs) in the ovary and the intestinal stem cells (ISCs) in the posterior midgut. Several themes have emerged from these studies, which suggest that there may be a common set of features among niches in a variety of epithelia. For example, unlike the simpler Drosophila germline stem cell niches, both the FSC and ISC niches produce multiple, partially redundant, niche signals, some of which activate pathways such as Wnt/Wingless, Hedgehog, and EGF that also regulate mammalian epithelial tissue renewal. Further study into these relatively new stem cell models will be of use in understanding both the specifics of epithelial regeneration and the diversity of mechanisms that regulate adult stem cells in general.

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Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation

Abstract: The Boi receptor regulates stem cell function by sequestering the diffusible hedgehog ligand.

Cited by 70 publications

References 53 publications

Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation

Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation

Genetic Interaction Screens Identify a Role for Hedgehog Signaling in Drosophila Border Cell Migration

Drosophila models of epithelial stem cells and their niches

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