<i>Drosophila</i> Ana1 is required for centrosome assembly and centriole elongation

Saurya, Saroj; Roque, Hélio; Novak, Zsofia A.; Wainman, Alan; Aydogan, Mustafa G.; Volanakis, Adam; Sieber, Boris; Pinto, David Miguel Susano; Raff, Jordan W.

doi:10.1242/jcs.186460

Cited by 39 publications

(60 citation statements)

References 76 publications

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“…It will be interesting to investigate whether Cep295 molecules on the mother centriole are rearranged and moved to the assembly site of a procentriole to trigger centriole formation. It should also be noted that while this study was under review, another studies reported that Cep295 and Ana1 regulate centriole elongation in human42 and Drosophila cells43, respectively.…”

Section: Discussionmentioning

confidence: 90%

Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole

et al. 2016

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Centrioles surrounded by pericentriolar material (PCM) serve as the core structure of the centrosome. A newly formed daughter centriole grows into a functional mother centriole. However, the underlying mechanisms remain poorly understood. Here we show that Cep295, an evolutionarily conserved protein, is required for generation of a bona fide mother centriole organizing a functional centrosome. We find that Cep295 is recruited to the proximal centriole wall in the early stages of procentriole assembly. Cep295 then acts as a scaffold for the proper assembly of the daughter centriole. We also find that Cep295 binds directly to and recruits Cep192 onto the daughter centriole wall, which presumably endows the function of the new mother centriole for PCM assembly, microtubule-organizing centre activity and the ability for centriole formation. These findings led us to propose that Cep295 acts upstream of the conserved pathway for centriole formation and promotes the daughter-to-mother centriole conversion.

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Section: Discussionmentioning

confidence: 90%

Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole

et al. 2016

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“…Centriole length can also be modulated by deregulation of proteins implicated in building the distal halves of centrioles, including the WD40 protein POC1 ( P roteome O f C entriole protein 1) 55 , the centrin-binding protein hPOC5 56 , or the microtubule binding protein CEP295 (Ana1 in Drosophila ) 57,58 . Interestingly, depletion of CEP295 not only impairs the recruitment of hPOC5 and POC1, but also blocks the acetylation and glutamylation of centriolar microtubules 57 .…”

Section: Centrosome Structure and Assemblymentioning

confidence: 99%

“…Best described are events in Drosophila , where PLK1 is first recruited to CPAP, through CDK1-dependent phosphorylation of a single docking site 92 . In both Drosophila and mammalian cells, PLK1 then triggers the sequential assembly of CEP135, CEP295/Ana1 and CEP152/Asl and downstream PCM formation 58,89,90 . Importantly, recruitment of Asl in Drosophila embryos only occurs after disengagement, indicating that these licensing processes occur sequentially 93 .…”

Section: Control Of Centriole Numbermentioning

confidence: 99%

Once and only once: mechanisms of centriole duplication and their deregulation in disease

Nigg

Holland

2018

Nat Rev Mol Cell Biol

407

546

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Centrioles are conserved microtubule-based organelles that form the core of the centrosome and act as templates for the formation of cilia and flagella. Centrioles have important roles in most microtubule related processes, including motility, cell division and cell signaling. To coordinate these diverse cellular processes, centriole number must be tightly controlled. In cycling cells, one new centriole is formed next to each preexisting centriole in every cell cycle. Advances in imaging, proteomics, structural biology and genome editing have revealed new insights into centriole biogenesis, how centriole numbers are controlled and how alterations in these structures contribute to diseases such as cancer and neurodevelopmental disorders. Moreover, recent work has uncovered the existence of surveillance pathways that limit proliferation of cells with numerical centriole aberrations. Here we discuss recent progress in this field with a focus on signaling pathways and molecular mechanisms.

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“…This function depends on the Ana1 N-terminal region (Saurya et al 2016), which was reported to interact with Cep135 in the process of centriole-to-centrosome conversion in cultured cells (Fu et al 2016). Additionally, Cep135, Ana1, and Asl also affect centriole elongation in vivo evoking the question of whether or not the two pathways may be interconnected.…”

Section: Centriole Elongationmentioning

confidence: 99%

The Centrioles,Centrosomes, Basal Bodies, and Cilia ofDrosophila melanogaster

Lattao¹,

Kovács²,

Glover

2017

Genetics

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Centrioles play a key role in the development of the fly. They are needed for the correct formation of centrosomes, the organelles at the poles of the spindle that can persist as microtubule organizing centers (MTOCs) into interphase. The ability to nucleate cytoplasmic microtubules (MTs) is a property of the surrounding pericentriolar material (PCM). The centriole has a dual life, existing not only as the core of the centrosome but also as the basal body, the structure that templates the formation of cilia and flagellae. Thus the structure and functions of the centriole, the centrosome, and the basal body have an impact upon many aspects of development and physiology that can readily be modeled in Drosophila. Centrosomes are essential to give organization to the rapidly increasing numbers of nuclei in the syncytial embryo and for the spatially precise execution of cell division in numerous tissues, particularly during male meiosis. Although mitotic cell cycles can take place in the absence of centrosomes, this is an error-prone process that opens up the fly to developmental defects and the potential of tumor formation. Here, we review the structure and functions of the centriole, the centrosome, and the basal body in different tissues and cultured cells of Drosophila melanogaster, highlighting their contributions to different aspects of development and cell division.

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Drosophila Ana1 is required for centrosome assembly and centriole elongation

Cited by 39 publications

References 76 publications

Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole

Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole

Once and only once: mechanisms of centriole duplication and their deregulation in disease

The Centrioles,Centrosomes, Basal Bodies, and Cilia ofDrosophila melanogaster

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