Dendrobiumliquor eliminates free radicals and suppresses cellular proteins expression disorder to protect cells from oxidant damage

Abstract

Dendrobium liquor obtained by soaking Dendrobium in Chinese liquor is considered as a health drink in China. Here, we found the pretreatment of extract of Dendrobium nobile Lindl. liquor (DNLE) attenuated the oxidative damage to cells caused by H 2 O 2 , while the abilities of DNLE of eliminating extracellular free radicals and promoting the activities of intracellular antioxidant enzymes were observed. Quantitative proteomics identified 375 differentially expressed proteins caused by H 2 O 2 treatment in 293T cells. However, only 12 differentially expressed proteins were found in DNLEpretreated cells which under the same oxidative damage. This suggested that the pretreatment of DNLE could suppress the disorder of protein expressions caused by oxidative stress which could induce cell death. Besides, DNLE was helpful for avoiding the unfolded protein response (UPR) and cell cycle disorder caused by oxidative stress. Taken together, these results demonstrated that Dendrobium liquor could be a healthy herbal drink with antioxidant function. Practical applications Dendrobium is used as an edible herb and a tonic food in traditional Chinese medicine. Dendrobium liquor obtained by soaking Dendrobium with Chinese liquor is also regarded as a nourishing health drink. However, there is rare research data on biological activity of Dendrobium liquor. Our current results demonstrated that the extract of Dendrobium nobile Lindl. liquor (DNLE) possessed the ability of eliminating free radicals in/out the human cells. More importantly, DNLE could help cells to resist the interference on cell life activities caused by oxidative stress. Since many evidences suggested that oxidative stress is linked to human disease and aging, and chemical antioxidant has some side effects on health, Dendrobium liquor can serve as a natural health drink with antioxidant function. Furthermore, the active ingredients in DNLE also possess the potential to be developed as natural antioxidant additive in food and cosmetics.

Various Fractions of Alcoholic Extracts from Dendrobium nobile Functionalized Antioxidation and Antiaging in D-Galactose-Induced Aging Mice

“…Aging is a complex and progressive biological process involving a wide range of chronic alterations in organs and tissues, including physiological and pathological changes, and is associated with multiple diseases, such as diabetes, cardiovascular disease, neurological diseases and cancer, and other geriatric syndromes [1][2][3][4]. The free radical oxidative stress (ROS) doctrine has become one of the most convincing theories of aging [5]. The accumulation of ROS under oxidative stress conditions leads to the induction of lipid peroxidation, resulting in advanced lipid oxidation end products (ALEs).…”

Dendrobium nobile Lindl: A Review on Its Chemical Constituents and Pharmacological Effects

“…Moreover, DNLE was also capable of mitigating the unfolded protein response and cell cycle disorder caused by oxidative stress. [42]…”

“…Moreover, DNLE was also capable of mitigating the unfolded protein response and cell cycle disorder caused by oxidative stress. [42] Hypoglycemic effect Metabolic syndrome is a common chronic disease characterized by obesity, dyslipidemia, raised blood pressure, and high glucose levels. MS significantly augments the risk of type 2 diabetes and adversely affects human health.…”

Opposite trends of glycosides and alkaloids in Dendrobium nobile of different age based on UPLC‐Q/TOF‐MS combined with multivariate statistical analyses

“…Pharmacological research confirmed that D. nobile improves cognitive dysfunction, reduces gastric damage, has anti‐tumor effects, and can be used to treat diabetes 4 . Due to its beneficial effects, D. nobile is also used as a tonic and functional food, such as Dendrobii Liquor 5 . Broad applications increased the demand for D. nobile and hence resulted in a sharp decline of wild D. nobile populations.…”

“…4 Due to its beneficial effects, D. nobile is also used as a tonic and functional food, such as Dendrobii Liquor. 5 Broad applications increased the demand for D. nobile and hence resulted in a sharp decline of wild D. nobile populations. In the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II, wild D. nobile is included.…”