<i>Deinococcus mumbaiensis</i>sp. nov., a radiation-resistant pleomorphic bacterium isolated from Mumbai, India

Shashidhar, Ravindranath; Bandekar, Jayant R.

doi:10.1111/j.1574-6968.2005.00033.x

Cited by 23 publications

(14 citation statements)

References 20 publications

(41 reference statements)

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“…For this reason, UV or gamma radiation has been used to isolate most organisms of the genus Deinococcus (Rainey et al, 2005;Oyaizu et al, 1987). However, a few novel species have been isolated from non-irradiated samples from air, soil, hot springs and culture contamination (Ferreira et al, 1997;Lai et al, 2006;Shashidhar & Bandekar, 2006;Weon et al, 2007). Isolation of strains Ho-08 T and PB314 T , with relatively low resistance to radiation, demonstrates that the extreme radiation resistance of the organisms is not a result of selection of resistant strains by irradiation but is a normal characteristic, as defined by Mattimore & Battista (1996).…”

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“…Although the 16S rRNA gene sequence similarity may be considered low enough to differentiate some species into other genera, the genus Deinococcus has not been divided because of the common characteristics of its members. Species of the genus Deinococcus are strictly aerobic, have high resistance to ionizing radiation, produce reddish, pink or yellow colonies, generally stain Gram-positive, have L-ornithine in the peptidoglycan and lack teichoic acids (Brooks & Murray, 1981;Ferreira et al, 1997;Rainey et al, 1997Rainey et al, , 2005Hirsch et al, 2004;Suresh et al, 2004;de Groot et al, 2005;Lai et al, 2006;Shashidhar & Bandekar, 2006;Weon et al, 2007). 16S rRNA gene sequence analyses show that the deinococci form a phylogenetically diverse group in a deeply branching lineage within the Bacteria (Rainey et al, 1997(Rainey et al, , 2005.…”

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Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge

Jung

Ten

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge The taxonomic positions of two environmental isolates from South Korea were established using a combination of genotypic and phenotypic data. The organisms, designated PB314 T and Ho-08 T , were Gram-negative, rod-shaped and non-spore-forming and had chemotaxonomic properties consistent with their classification in the genus Deinococcus 16S rRNA gene tree, the highest sequence similarities being shown to the type strains of Deinococcus grandis (96.3-96.7 %) and Deinococcus indicus (96.3-96.4 %). The isolates shared relatively high 16S rRNA gene sequence similarity (98.1 %) but had a DNA-DNA relatedness value of only 22 %. Chemotaxonomic data revealed that both strains possess quinone system MK-8 as the predominant compound, C 16 : 1 v7c and C 16 : 0 as major fatty acids and ornithine as a diamino acid in the peptidoglycan structure, corroborating our assignment of the strains to the genus Deinococcus. The results of phylogenetic analyses based on 16S rRNA gene sequences, DNA-DNA relatedness values and physiological and biochemical tests clearly demonstrated that the two strains represent distinct species. On the basis of these data, two novel species, Deinococcus aquaticus sp. nov. (type strain PB314 T 5KCTC 12552 T 5NBRC 101311 T ) and Deinococcus caeni sp. nov. (type strain Ho-08 T 5KCTC 12553 T 5NBRC 101312 T ), are proposed.The availability of improved taxonomic procedures facilitates the preliminary characterization of relatively large numbers of bacteria from environmental samples and hence the recognition of novel species. Over 100 putatively novel species were highlighted in our laboratory following the characterization of 300 aerobic phototrophic bacteria isolated from water and sediment samples collected either from the River Gapcheon, near Daejeon City, South Korea, or from a tributary of the river. One of the isolates, strain PB314, was tentatively assigned to the genus Deinococcus (unpublished results). A second strain, isolate Ho-08, was also considered to belong to the genus Deinococcus following the characterization of 128 bacterial strains isolated from activated sludge, compost and soil collected in the vicinity of Daejeon City in a second study designed to find novel bacterial species (Im et al., 2003).The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains PB314 T and Ho-08 T are respectively DQ017708 and DQ017709.A 16S rRNA gene sequence-based maximum-likelihood tree, results of 2D TLC of polar lipids of strains PB314 T and Ho-08 T and a comparison of fatty acid profiles are available as supplementary material with the online version of this paper.

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Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge

Jung

Ten

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…These species have been isolated from a wide range of environments, e.g. desert soil (Rainey et al, 2005;de Groot et al, 2005), aquifers (Suresh et al, 2004), the plant rhizosphere (Lai et al, 2006), hot springs (Ferreira et al, 1997) and airborne dust (Shashidhar & Bandekar, 2006;Weon et al, 2007). Most members of the genus are strictly aerobic, have optimum growth temperatures in the range 25-35 u C and form red, pink, light-pink or reddish colonies.…”

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Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium

Yuan

Zhang

Dai

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain I-0 T , was isolated from a sand sample of the Gobi desert in Xinjiang Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this isolate represents a novel member of the genus Deinococcus, with low sequence similarities (,94 %) to recognized Deinococcus species. The major cellular fatty acids were C 16 : 1 v7c and C 16 : 0 . Its polar lipid profile contained several unidentified glycolipids, phosphoglycolipids, phospholipids, pigments and an aminophospholipid. The peptidoglycan type was Orn-Gly 2 (A3b) and the predominant respiratory quinone was MK-8. The DNA G+C content was 65.4 mol%. DNA-DNA relatedness between strain I-0 T and Deinococcus radiodurans ACCC 10492 T was 37 %. The strain was shown to be extremely resistant to gamma radiation (.15 kGy) and UV light (.600 J m "2 ). On the basis of the phylogenetic, chemotaxonomic and phenotypic data presented, strain I-0 T represents a novel species of the genus Deinococcus, for which the name Deinococcus gobiensis sp. nov. is proposed. The type strain is I-0 T (5DSM 21396 T 5CGMCC 1.7299 T ).The genus Deinococcus, which was described by Brooks & Murray (1981) ) and desiccation (years) is a distinctive characteristic of this genus (Makarova et al., 2007). This resistance has been attributed to a highly proficient DNA repair system, and it seems likely that radiation resistance evolved as a consequence of chronic exposure to non-radioactive forms of DNA damage, such as desiccation (Makarova et al., 2001).In the course of the study of stress-resistant bacteria from arid environments, a novel Deinococcus isolate was obtained from the upper sand layers of the Gobi desert, Xinjiang, China, where bacteria are exposed to cycles of high and low temperatures and to prolonged desiccation. In this paper, we report on the taxonomic characterization of this radiation-resistant, red-coloured strain, designated I-0 T , which was obtained from a mixed sand sample. After exposure of the sample to 10 kGy gamma radiation from a 60 Co source (CAIC), it was enriched in 50 ml TGY medium (1.0 % peptone, 0.5 % yeast extract, 0.1 % glucose) at 30 u C with shaking at 200 r.p.m. for up to 5 days, followed by isolation of surviving red-colony-forming bacteria on TGY agar plates (TGY medium with 1.5 % agar).Morphology of cells grown for 24-48 h on TGY agar was examined by zoom stereo microscopy (model SZX7; Olympus), light microscopy (model BX-51; Olympus), scanning electron microscopy (model S-570; Hitachi) and transmission electron microscopy (model H-7500; Hitachi). Gram staining was carried out using the modified 3These authors contributed equally to this work.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain I-0 T is EU427464.Micrographs of colonies and cells of strain I-0 T , a 16S rRNA gene sequence-based maximum-parsimony tree, the fatty acid profile of strain I-0 T and a table of 16S rRNA gene sequence s...

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“…The taxonomy of the genus has been described extensively (Suresh et al, 2004;Hirsch et al, 2004;Ferreira et al, 1997;Rainey et al, 1997Rainey et al, , 2005, and the number of species is still growing (Asker et al, 2009;Callegan et al, 2008;de Groot et al, 2005;Lai et al, 2006;Rainey et al, 2007;Shashidhar & Bandekar, 2006;Zhang et al, 2007). Several novel Deinococcus strains have been isolated and well characterized from soils, including desert soil, foods, faeces and dust, with additional data on their extreme resistance to UV and gamma radiation and desiccation.…”

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Deinococcus aquatilis sp. nov., isolated from water

Kämpfer

Lodders

Huber³

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A pale-pink strain (CCUG 53370 T ) from water was investigated by a polyphasic taxonomic approach. The cells stained Gram-positive and were rod-shaped and non-spore-forming. Analyses using the 16S rRNA gene sequence of the isolate showed that the organism belongs to the genus Deinococcus, with the highest sequence similarities to the type strains of Deinococcus ficus (94.4 %), Deinococcus navajonensis (94.3 %) and Deinococcus mumbaiensis (94.3 %). Chemotaxonomic data revealed that CCUG 53370 T contains exclusively menaquinone MK-8 as the respiratory quinone and a complex polar lipid profile consisting of different unidentified glycolipids and polar lipids, two unknown phospholipids and three unknown phosphoglycolipids. As in other deinococci, one of these phosphoglycolipids was predominant in the profile, and it was identified in Deinococcus radiodurans as 29-O-(1,2-diacyl-sn-glycero-3-phospho)-39-O-(agalactosyl)-N-D-glyceroyl alkylamine. Predominant fatty acids were C 16 : 1 v7c, C 17 : 1 v8c and C 17 : 1 v9c. Biochemical and chemotaxonomic properties demonstrate that strain CCUG 53370 T represents a novel species, for which the name Deinococcus aquatilis sp. nov. is proposed. The type strain is CCUG 53370 T (5CCM 7524 T ).

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Deinococcus mumbaiensissp. nov., a radiation-resistant pleomorphic bacterium isolated from Mumbai, India

Cited by 23 publications

References 20 publications

Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge

Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge

Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium

Deinococcus aquatilis sp. nov., isolated from water

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