CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis

Liu, Jun; Deng, Shulin; Wang, Huan; Jian, Ye; Wu, Hui‐Wen; Sun, Hai‐Xi; Chua, Nam‐Hai

doi:10.1104/pp.15.01335

Cited by 62 publications

(59 citation statements)

References 76 publications

(142 reference statements)

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“…This energetic trade-off has been observed in genetic mutants such as APETALA2 495051 and CURLY LEAF28 52. In this study we showed that manipulation of the auxin pathway in J.curcas not only increased seed size but also enhanced total seed yield.…”

Section: Discussionsupporting

confidence: 73%

Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas

Sun

Wang

et al. 2017

Sci Rep

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Seed size is a major determinant of seed yield but few is known about the genetics controlling of seed size in plants. Phytohormones cytokinin and brassinosteroid were known to be involved in the regulation of herbaceous plant seed development. Here we identified a homolog of Auxin Response Factor 19 (JcARF19) from a woody plant Jatropha curcas and genetically demonstrated its functions in controlling seed size and seed yield. Through Virus Induced Gene Silencing (VIGS), we found that JcARF19 was a positive upstream modulator in auxin signaling and may control plant organ size in J. curcas. Importantly, transgenic overexpression of JcARF19 significantly increased seed size and seed yield in plants Arabidopsis thaliana and J. curcas, indicating the importance of auxin pathway in seed yield controlling in dicot plants. Transcripts analysis indicated that ectopic expression of JcARF19 in J. curcas upregulated auxin responsive genes encoding essential regulators in cell differentiation and cytoskeletal dynamics of seed development. Our data suggested the potential of improving seed traits by precisely engineering auxin signaling in woody perennial plants.

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Section: Discussionsupporting

confidence: 73%

Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas

Sun

Wang

et al. 2017

Sci Rep

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“…6). This is resonant with the recent report in mouse that re-establishment of H3K4me3 occurs more rapidly than that of H3K27me3 during activation of the zygote genome [Liu, 2016. In mammalian cells, deposition of H3K27me3 by PcG proteins is hindered by the presence of H3K4me3 [Schmitges, 2011.…”

Section: Integrative Analysis Of Chromatin and Transcription Datasetssupporting

confidence: 60%

Dynamics of H3K4me3 Chromatin Marks Take the Lead over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsis thaliana

Engelhorn¹,

Blanvillain²,

Kröner³

et al. 2017

Preprint

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Plant life-long organogenesis involves sequential, time and tissue specific expression of developmental genes. This requires activities of Polycomb Group (PcG) and trithorax Group complexes, respectively responsible for repressive Histone 3 trimethylation at lysine 27 (H3K27me3) and activation-related H3K4me3. However, the genome-wide dynamics in histone modifications that occur during developmental processes have remained elusive. Here, we report the distributions of H3K27me3 and H3K4me3 along with transcriptional changes, in a developmental series including Arabidopsis leaf and three stages of flower development. We found that chromatin mark levels are highly dynamic over the time series on nearly half of all Arabidopsis genes. Moreover, during early flower morphogenesis, changes in H3K4me3 prime over changes in H3K27me3 and quantitatively correlate with transcription changes, while H3K27me3 changes occur after prolonged expression changes. Notably, early activation of PcG target genes is dominated by increases in H3K4me3 while H3K27me3 remains present at the locus. Our results reveal H3K4me3 as greater predictor over H3K27me3 for transcription dynamics, unveil unexpected chromatin mechanisms at gene activation and underline the relevance of tissue-specific temporal epigenomics.

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“…A similar result of higher intronic transcript levels was found in clf‐28 shoot (Fig. S1c) (Liu et al ., ).…”

Section: Resultsmentioning

confidence: 97%

A noncoding RNA transcribed from the AGAMOUS (AG) second intron binds to CURLY LEAF and represses AG expression in leaves

Deng

et al. 2018

New Phytologist

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Dispersed H3K27 trimethylation (H3K27me3) of the AGAMOUS (AG) genomic locus is mediated by CURLY LEAF (CLF), a component of the Polycomb Repressive Complex (PRC) 2. Previous reports have shown that the AG second intron, which confers AG tissue-specific expression, harbors sequences targeted by several positive and negative regulators. Using RACE reverse transcription polymerase chain reaction, we found that the AG intron 2 encodes several noncoding RNAs. RNAi experiment showed that incRNA4 is needed for CLF repressive activity. AG-incRNA4RNAi lines showed increased leaf AG mRNA levels associated with a decrease of H3K27me3 levels; these plants displayed AG overexpression phenotypes. Genetic and biochemical analyses demonstrated that the AG-incRNA4 can associate with CLF to repress AG expression in leaf tissues through H3K27me3-mediated repression and to autoregulate its own expression level. The mechanism of AG-incRNA4-mediated repression may be relevant to investigations on tissue-specific expression of Arabidopsis MADS-box genes.

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CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis

Cited by 62 publications

References 76 publications

Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas

Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas

Dynamics of H3K4me3 Chromatin Marks Take the Lead over H3K27me3 for Gene Regulation during Flower Morphogenesis in <em>Arabidopsis</em> <em>thaliana</em>

A noncoding RNA transcribed from the AGAMOUS (AG) second intron binds to CURLY LEAF and represses AG expression in leaves

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