A noncoding <scp>RNA</scp> transcribed from the <scp>AGAMOUS</scp> (<scp>AG</scp>) second intron binds to <scp>CURLY LEAF</scp> and represses <scp>AG</scp> expression in leaves

Wu, Hui‐Wen; Deng, Shulin; Xu, Hui; Mao, Hui-Zhu; Liu, Jun; Niu, Qi-Wen; Wang, Huan; Chua, Nam‐Hai

doi:10.1111/nph.15231

Cited by 72 publications

(58 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Knockdown of AG-lincRNA4 resulted in AG activation in leaves by lowering the H3K27me3 level at the AG locus. Consequently, the corresponding mutant showed phenotypes resembling those of ectopic AG expression (Wu et al 2018 ). LDMAR was identified in rice through map-based cloning and regulates photoperiod-sensitive male fertility via RdDM (Ding et al 2012a , b ; Zhou et al 2012 ).…”

Section: Functions and Molecular Mechanisms Of Lncrnas In Plantsmentioning

confidence: 99%

Long non-coding RNAs in plants: emerging modulators of gene activity in development and stress responses

Chen

Zhu

Kaufmann

2020

Planta

View full text Add to dashboard Cite

Main conclusion Long non-coding RNAs modulate gene activity in plant development and stress responses by various molecular mechanisms. Abstract Long non-coding RNAs (lncRNAs) are transcripts larger than 200 nucleotides without protein coding potential. Computational approaches have identified numerous lncRNAs in different plant species. Research in the past decade has unveiled that plant lncRNAs participate in a wide range of biological processes, including regulation of flowering time and morphogenesis of reproductive organs, as well as abiotic and biotic stress responses. LncRNAs execute their functions by interacting with DNA, RNA and protein molecules, and by modulating the expression level of their targets through epigenetic, transcriptional, post-transcriptional or translational regulation. In this review, we summarize characteristics of plant lncRNAs, discuss recent progress on understanding of lncRNA functions, and propose an experimental framework for functional characterization.

show abstract

Section: Functions and Molecular Mechanisms Of Lncrnas In Plantsmentioning

confidence: 99%

Long non-coding RNAs in plants: emerging modulators of gene activity in development and stress responses

Chen

Zhu

Kaufmann

2020

Planta

View full text Add to dashboard Cite

show abstract

“…Based on ChIP, ChIP-qPCR, RIP, and other experiments, several lncRNAs mentioned above, including COOLAIR, COLDAIR, APOLO, ELENA, DRIR, SVALKA, ASCO-lncRNA, MAS and LDMAR, are all classically regulated through epigenetic regulation. Moreover, Wu et al [69] found that the methylation level of H3K27me3 in Arabidopsis AG-incRNA4 RNAi was significantly lower than that of the control by performing ChIP-seq. The RNA-pulldown and RIP techniques were used to further reveal that the sense strand of AG-incRNA4 can directly bind to the CURLY LEAF (CLF) protein.…”

Section: Epigenetic Regulation Of Lncrnamentioning

confidence: 99%

Research Progress on Plant Long Non-Coding RNA

Liu

et al. 2020

Plants

View full text Add to dashboard Cite

Non-coding RNAs (ncRNAs) that were once considered “dark matter” or “transcriptional noise” in genomes are research hotspots in the field of epigenetics. The most well-known microRNAs (miRNAs) are a class of short non-coding, small molecular weight RNAs with lengths of 20–24 nucleotides that are highly conserved throughout evolution. Through complementary pairing with the bases of target sites, target gene transcripts are cleaved and degraded, or translation is inhibited, thus regulating the growth and development of organisms. Unlike miRNAs, which have been studied thoroughly, long non-coding RNAs (lncRNAs) are a group of poorly conserved RNA molecules with a sequence length of more than 200 nucleotides and no protein encoding capability; they interact with large molecules, such as DNA, RNA, and proteins, and regulate protein modification, chromatin remodeling, protein functional activity, and RNA metabolism in vivo through cis- or trans-activation at the transcriptional, post-transcriptional, and epigenetic levels. Research on plant lncRNAs is just beginning and has gradually emerged in the field of plant molecular biology. Currently, some studies have revealed that lncRNAs are extensively involved in plant growth and development and stress response processes by mediating the transmission and expression of genetic information. This paper systematically introduces lncRNA and its regulatory mechanisms, reviews the current status and progress of lncRNA research in plants, summarizes the main techniques and strategies of lncRNA research in recent years, and discusses existing problems and prospects, in order to provide ideas for further exploration and verification of the specific evolution of plant lncRNAs and their biological functions.

show abstract

“…Adding 35S enhancer can extend the tissue specificity of NtAGIP1 from inner whorls of flower (stamens and carpels) to the outer whorls (sepals and petals), but the activity of 35SNtA-GIP1 does not cross the boundary of flower organs. An array of factors is responsible for the repression of AG [44,45]. For example, APETALA2 is involved in the repressing the expression of AG in the outer floral whorls through recruiting the histone deacetylase HDA19, thus…”

Section: Plos Onementioning

confidence: 99%

“…Importantly, GAGA factor binding motif has been found to be involved in the recruitment of PRC2 in both Arabidopsis and Drosophila. Recently, Wu and colleagues reported that a DNA region within Arabidopsis AG intron 2 (+2616 to +3348) is involved in the recruitment of PRC2 to represses AG expression in leaves via the transcribed noncoding RNA [45]. They pointed out that the identified DNA region also contains PREs which may recruit PRC2.…”

Section: Plos Onementioning

confidence: 99%

A 100 bp GAGA motif-containing sequence in AGAMOUS second intron is able to suppress the activity of CaMV35S enhancer in vegetative tissues

Liu¹,

Zou

Wang³

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

Flower-specific promoters enable genetic manipulation of floral organs to improve crop yield and quality without affecting vegetative growth. However, the identification of strong tissuespecific promoters is a challenge. In addition, information on cis elements that is able to repress gene expression in vegetative tissues remains limited. Here, we report that fusing a 35S enhancer to the stamen-and carpel-specific NtAGIP1 promoter derived from the tobacco AGAMOUS second intron (AGI) can significantly increase the promoter activity. Interestingly, although the activity of the new promoter extends to sepals and pedicles, it does not cross the boundary of the reproductive organs. Serial deletion of the AGI and chromatin immunoprecipitation (ChIP) assay reveal a 100-bp fragment that contains a conserved GAGA factor binding motif contributes to the flower specificity by mediating histone H3 lysine 27 trimethylation (H3K27me3) modification of the promoter. Furthermore, this fragment shows significant suppressive effect on the activity of the 35S enhancer in vegetative tissues, consequently, resulting in a significant increase of the activity of 35S enhancer: AGI chimeric promoter without sacrifice of its specificity in inflorescence. A 100 bp GAGA motif-containing sequence in AGAMOUS second intron is able to suppress the activity of CaMV35S enhancer in vegetative tissues. PLoS ONE 15(3): e0230203. https://doi.org/ 10.The plasmid pBI121 [29] that contain CaMV35S::GUS was used as backbone to construct vectors used for plant transformation in this study. The second intron of NtAG-1 gene was fused with a 45 bp minimal 35S promoter to create a functional NtAGIP1 promoter [28]. NtAGIP1:: PLOS ONEA GAGA-containing sequence in AGAMOUS intron suppress the activity of CaMV35S enhancer in vegetative tissues PLOS ONE | https://doi.org/10.A GAGA-containing sequence in AGAMOUS intron suppress the activity of CaMV35S enhancer in vegetative tissues PLOS ONE | https://doi.org/10. Formal analysis: Ruochen Liu.Funding acquisition: Yan Pei.Investigation: Ruochen Liu, Xiuping Zou, You Wang, Qin Long. Fig 8. ChIP assay for H3K27me3 levels in NtAGI-1 intron and chimeric promoters. (A) ChIP assay for H3K27me3 levels at the NtAGI-1 intron in leaves of wild-type tobacco. (B) ChIP assay for H3K27me3 levels in leaves of the -2835 and -2735 lines. The genomic fragment from immunoprecipitation for qRT-PCR detection is depicted as black horizontal line. Enrichment was represented as percentage of Input (% Input). Error bars represent the standard deviation of three biological replicates.A GAGA-containing sequence in AGAMOUS intron suppress the activity of CaMV35S enhancer in vegetative tissues PLOS ONE | https://doi.org/10.

show abstract

A noncoding RNA transcribed from the AGAMOUS (AG) second intron binds to CURLY LEAF and represses AG expression in leaves

Cited by 72 publications

References 48 publications

Long non-coding RNAs in plants: emerging modulators of gene activity in development and stress responses

Long non-coding RNAs in plants: emerging modulators of gene activity in development and stress responses

Research Progress on Plant Long Non-Coding RNA

A 100 bp GAGA motif-containing sequence in AGAMOUS second intron is able to suppress the activity of CaMV35S enhancer in vegetative tissues

Contact Info

Product

Resources

About