<i>Cryptococcus gattii</i> VGII in a <i>Plathymenia reticulata</i> hollow in Cuiabá, Mato Grosso, Brazil

Anzai, Mariana Caselli; Lazéra, Márcia dos Santos; Wanke, Bodo; Trilles, Luciana; Dutra, Valéria; Paula, Daphine Ariadne Jesus de; Nakazato, Luciano; Takahara, Doracilde Terumi; Simi, Walquirya Borges; Hahn, Rosane Christine

doi:10.1111/myc.12177

Cited by 17 publications

(22 citation statements)

References 29 publications

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“…The genotype VGII (25/56, 44.6%) was the most frequently isolated, as reported previously [36][37][38][39][40][41][42], and in agreement with this work. Different results have been observed in southern Brazil, with Casali et al [18] reporting 11 isolates of C. gattii serotype B type VGIII among the clinical samples they studied.…”

Section: Discussionsupporting

confidence: 80%

“…This outbreak indicated that exposure to environmental sources, such as trees and soil contaminated by this yeast, can lead to cryptococcosis infection in humans and animals [38,39,40]. In a study analyzing environmental samples in Cuiabá, MT, Anzai et al [41] also isolated the genotype C. gattii VGII, which is similar to that detected in the clinical isolates analyzed in this work.…”

Section: Discussionsupporting

confidence: 57%

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Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil

Favalessa

Paula

Dutra

et al. 2014

J Infect Dev Ctries

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Introduction: Cryptococcosis is a systemic fungal infection that affects humans and animals, mainly due to Cryptococcus neoformans and Cryptococcus gattii. Following the epidemic of acquired immunodeficiency syndrome (AIDS), fungal infections by C. neoformans have become more common among immunocompromised patients. Cryptococcus gattii has primarily been isolated as a primary pathogen in healthy hosts and occurs endemically in northern and northeastern Brazil. We to perform genotypic characterization and determine the in vitro susceptibility profile to antifungal drugs of the Cryptococcus species complex isolated from HIV-positive and HIV-negative patients attended at university hospitals in Cuiabá, MT, in the Midwestern region of Brazil. Methodology: Micromorphological features, chemotyping with canavanine-glycine-bromothymol blue (CGB) agar and genotyping by URA5-RFLP were used to identify the species. The antifungal drugs tested were amphotericin B, fluconazole, flucytosine, itraconazole and voriconazole. Minimum inhibitory concentrations (MICs) were determined according to the CLSI methodology M27-A3. Results: Analysis of samples yelded C. neoformans AFLP1/VNI (17/27, 63.0%) and C. gattii AFLP6/VGII (10/27, 37.0%). The MICs ranges for the antifungal drugs were: amphotericin B (0.5-1 mg/L), fluconazole (1-16 mg/L), flucytosine (1-16 mg/L), itraconazole (0.25-0.12 mg/L) and voriconazole (0.06-0.5 mg/L). Isolates of C. neoformans AFLP1/VNI were predominant in patients with HIV/AIDS, and C. gattii VGII in HIV-negative patients. The genotypes identified were susceptible to the antifungal drugs tested. Conclusion: It is worth emphasizing that AFLP6/VGII is a predominant genotype affecting HIV-negative individuals in Cuiabá. These findings serve as a guide concerning the molecular epidemiology of C. neoformans and C. gattii in the State of Mato Grosso.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 57%

Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil

Favalessa

Paula

Dutra

et al. 2014

J Infect Dev Ctries

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“…Subsequent reports have shown that this association is not limited to Eucalyptus trees or a particular geographical area which contributed to a better understanding of the diversity of its natural sources that interestingly, do not include poultry manure . Early in this decade, the outbreak at the west coast of North America indicated a drastic change in the adaptation of this organism to other environments …”

Section: How Does the Infection Occur?contrasting

confidence: 84%

“…23,25,31,32 Early in this decade, the outbreak at the west coast of North America indicated a drastic change in the adaptation of this organism to other environments. 26,31,[33][34][35] Cuba is an archipelago located in the Caribbean Sea. The local climate is tropical with an average temperature of 23°C with abundant and varied flora and fauna.…”

Section: The Causative Agent Of Cryptococcosismentioning

confidence: 99%

Cryptococcus and Cryptococcosis in Cuba. A minireview

Illnait-Zaragozí

Martínez-Machín

Fernández-Andreu

et al. 2014

Mycoses

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Cryptococcosis has emerged as an important public health problem in Africa, Asia and the Americas due to the increasing numbers of persons at risk of this infection and the adaptation of its aetiological agents to new environments. The proper management requires early recognition of Cryptococcus neoformans/C. gattii species complex infection, familiarity with the use and limitations of diagnostic tests and knowledge of the available treatment options. This review will address these issues with the goal of providing sufficient information to suspect, diagnose and treat patients with cryptococcosis based on Cuban data and review of the literature.

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“…C. gattii AFLP6/VGII was also isolated in a study analysing environmental samples from tree hollows in Cuiabá, MT, conducted by Anzai et al . .…”

Section: Discussionmentioning

confidence: 99%

Fatal Cryptococcus gattii genotype AFLP6/VGII infection in a HIV‐negative patient: case report and a literature review

Favalessa

Lazéra

Wanke

et al. 2014

Mycoses

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Cryptococcus gattii, a species belonging to the Cryptococcus complex which occurs endemically in tropical and subtropical regions, has been reported as a causative agent of cryptococcosis in healthy individuals. We report a case of meningitis in HIV-negative patient from Cuiaba, MT, in the Midwestern region of Brazil. Cryptococcus gattii AFLP6/VGII was isolated from cerebrospinal fluid and molecular typing was performed by URA5-RFLP. The in vitro susceptibility profile was determined using the standard method according to the document M27A3, CLSI 2008. C. gattii AFLP6/VGII was shown to be susceptible to the antifungals tested. Treatment with 0.8 mg/kg of amphotericin B was initiated; however, the patient died 2 days after the onset of therapy.

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Cryptococcus gattii VGII in a Plathymenia reticulata hollow in Cuiabá, Mato Grosso, Brazil

Cited by 17 publications

References 29 publications

Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil

Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil

Cryptococcus and Cryptococcosis in Cuba. A minireview

Fatal Cryptococcus gattii genotype AFLP6/VGII infection in a HIV‐negative patient: case report and a literature review

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