<i>Coxiella burnetii</i>in Central Italy: Novel Genotypes Are Circulating in Cattle and Goats

Domenico, Marco Di; Curini, Valentina; Massis, Fabrizio De; Provvido, Andrea Di; Scacchia, Massimo; Cammà, Cesare

doi:10.1089/vbz.2014.1587

Cited by 16 publications

(29 citation statements)

References 39 publications

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“…Thus, to date the present work is the first report in Italy that confirms the presence of C. burnetii DNA in wild rodents. Genotyping of rodent-associated strains will be the next step in studying the ecology of Coxiella , considering the recent detection of novel genotypes circulating in the Abruzzi Region (Di Domenico et al 2014 ), and in assessing the risk of Q fever for livestock and humans.…”

Section: Discussionmentioning

confidence: 99%

“…Since then, however, only sporadic and scattered serosurveys have been conducted; therefore, limited information is available on the distribution of the etiological agent. Recently, a study on the spread and molecular characterization of the pathogen in a domestic cycle in central Italy revealed novel genotypes circulating in cattle and goats (Di Domenico et al 2014 ). However, no investigation was conducted in natural environment and the role of wild cycle in maintaining and spreading of the pathogen is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Lyme Disease and Q Fever Agents in Wild Rodents in Central Italy

Pascucci

Domenico

Dall'Acqua

et al. 2015

Vector-Borne and Zoonotic Diseases

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The maintenance of tick-borne disease agents in the environment strictly depends on the relationship between tick vectors and their hosts, which act as reservoirs for these pathogens. A pilot study aimed to investigate wild rodents as reservoirs for zoonotic tick-borne pathogens (Borrelia burgdorferi sensu lato (s.l.), Coxiella burnetii, Francisella tularensis, and Anaplasma phagocytophilum) was carried out in an area of Gran Sasso e Monti della Laga National Park (Abruzzi Region, central Italy), a wide protected area where, despite sporadic reports of infection in humans and animals, eco-epidemiological data on these diseases are still not available. Rodents were trapped and released at the capture site after the collection of feeding ticks and blood samples. In all, 172 ticks were collected; the most frequent species was Ixodes acuminatus (53%). Out of 88 tick pools, 11 resulted positive for C. burnetii and 13 for B. burgdorferi s.l.; the Borrelia afzelii genospecies was identified in one Ixodes ricinus tick collected from one Apodemus sp. rodent. Out of 143 blood samples, seven Apodemus spp. and five Myodes glareolus were positive for B. burgdorferi s.l. and two Apodemus spp. were positive for C. burnetii. All samples (ticks and blood) were negative for F. tularensis and A. phagocytophilum. This is the first report of B. burgdorferi s.l. in the environment for Abruzzi Region. Data on the presence of B. burgdorferi s.l. are similar to that observed in other Mediterranean countries. The present work is also the first report of C. burnetii in wild rodents in Italy. C. burnetii infection has been largely investigated in Italy in ruminant farms by serology and molecular methods, but information on ecology and on the wild cycle are still lacking. Further studies including genotyping should be performed and species-specific differences between wild rodent reservoirs of Q fever and Lyme disease agents should be investigated.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Lyme Disease and Q Fever Agents in Wild Rodents in Central Italy

Pascucci

Domenico

Dall'Acqua

et al. 2015

Vector-Borne and Zoonotic Diseases

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“…During the period 2012–2015 20 farms were positive for C. burnetii by Real Time PCR [ 21 ], with slight modifications [ 14 ]. Farms were named G1-G10 for goat, S1-S9 for sheep, and C1 for cow (Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…Multispacer Sequence Typing of C. burnetii DNA was performed on 20 specimens as previously described by Glazunova et al [ 12 ] with some modifications [ 14 ]. Raw sequence data were assembled using SeqMan Pro (DNASTAR Lasergene 10 Core Suite).…”

Section: Methodsmentioning

confidence: 99%

“…In 2005, typing via sequence analysis of multi-spacer regions (MST) was introduced by Glazunova et al [ 12 ], who identified 10 highly variable intergenic spacers allowing the unambiguous characterization of the agent. MST genotyping has high levels of discrimination and helps to trace the spread of C. burnetii from one region to another and to define phylogenetic relationships [ 13 , 14 ]. Otherwise, multi-locus Variable Number Tandem Repeat (VNTR) analysis (MLVA) was first established by Svraka et al [ 15 ], and then improved by Arricau-Bouvery et al [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

et al. 2018

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BackgroundAs the epidemiology of human Q Fever generally reflects the spread of Coxiella burnetii in ruminant livestock, molecular characterization of strains is essential to prevent human outbreaks. In this study we report the genetic diversity of C. burnetii in central Italy accomplished by MST and MLVA-6 on biological samples from 20 goat, sheep and cow farms.ResultsFive MST and ten MLVA profiles emerged from the analysis establishing a part of C. burnetii strain world atlas. In particular, ST32 occurred on 12 farms (60%), prevalently in goat specimens, while ST12 (25%) was detected on 4 sheep and 1 goat samples. ST8 and a variant of this genotype were described on 2 different sheep farms, whereas ST55 was observed on a goat farm. Five complete MLVA profiles different from any other published genotypes were described in this study in addition to 15 MLVA incomplete panels. Despite this, polymorphic markers Ms23, Ms24 and Ms33 enabled the identification of samples sharing the same MST profile.ConclusionsIntegration of such data in international databases can be of further help in the attempt of building a global phylogeny and epidemiology of Q fever in animals, with a “One Health” perspective.

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Characterization of bacterial communities in ticks parasitizing cattle in a touristic location in southwestern China

Xiang

Zhou

et al. 2023

Exp Appl Acarol

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The purpose of this study was to investigate tick species around Mount Fanjing and analyze bacterial communities in two species – Rhipicephalus microplus and Haemaphysalis longicornis – parasitizing cattle in Tongren, Guizhou province, Southwest China, using high-throughput sequencing methods. In April 2019, ticks were collected from five sites in Jiangkou County, Yinjiang County, and Songtao County. In total, 296 ticks were collected, comprising two genera and three species: H. longicornis, Haemaphysalis flava, and R. microplus. Rhipicephalus microplus was the most representative species (57.4%) within the collected group, being the dominant species in Tongren City, followed by H. longicornis (39.5%) and H. flava (3.0%). Beta-diversity analysis revealed differences in bacterial community composition among the tick species. The bacterial community structure of R. microplus collected in the three counties was highly similar. Chlorella and Bacillus were highly abundant in H. longicornis. Rickettsia was detected at high relative abundance in R. microplus but in low relative abundance in H. longicornis, suggesting that Rickettsia is more associated with R. microplus than with H. longicornis. More in-depth investigations are needed to determine the pathogenic risk of Rickettsia and its relationship with the host. This is the first survey on tick-borne bacterial communities in this area, which is of great significance for the prevention and control of tick-borne diseases locally.

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Coxiella burnetiiin Central Italy: Novel Genotypes Are Circulating in Cattle and Goats

Cited by 16 publications

References 39 publications

Detection of Lyme Disease and Q Fever Agents in Wild Rodents in Central Italy

Detection of Lyme Disease and Q Fever Agents in Wild Rodents in Central Italy

Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy

Characterization of bacterial communities in ticks parasitizing cattle in a touristic location in southwestern China

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