Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo

Abstract

Objectives. To investigate the anti-inflammatory effect of Coptis chinensis plus myrobalan (CM) in vitro and in vivo. Methods. The inflammation in mouse peritoneal macrophages was induced by lipopolysaccharide (LPS). Animal models were established by using ear swelling and paw edema of mouse induced by xylene and formaldehyde, respectively. In vitro, cytotoxicity, the phagocytosis of macrophages, the levels of nitric oxide (NO), induced nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in cell supernatant were detected. In vivo, swelling rate and edema inhibitory rate of ear and paw were observed using CM-treated mice. Results. At 150–18.75 μg·mL−1, CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS. In vivo, pretreatment with CM, the ear swelling, and paw edema of mice could be significantly inhibited in a dose-dependent manner, and the antiedema effect of CM at high dose was better than dexamethasone. Conclusion. Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases.

Electrospinning Fabrication of Poly(vinyl alcohol)/Coptis chinensis Extract Nanofibers for Antimicrobial Exploits

“…CC, which was the plant used in this research work, has also been studied by several other researchers to investigate its medicinal benefits and cosmetics. The anti-inflammatory influence of CC was reported in a research article [ 25 ]. In other research work, the metabolic interaction of some active constituents of CC in human liver microsomes was investigated [ 26 ].…”

Pharmacokinetics and tissue distribution of coptisine in rats after oral administration by liquid chromatography–mass spectrometry

“…Coptis chinensis Franch, of the Ranunculaceae family, which is commonly known as Huanglian in China, is one of the 50 fundamental herbs used in traditional Chinese medicine (Li, Tsai, Damu, & Wu, ). Coptidis rhizoma, the dried rhizome of C. chinensis , has commonly been prescribed for various clinical effects, such as anti‐diabetic (Jiang et al, ), hypocholesterolemic and hypoglycemic (Yuan, Tu, Ye, & Wu, ), anti‐inflammatory (Choi et al, ; Cui et al, ), antioxidant (Ban et al, ), analgesia (Tjong et al, ) and neuroprotective (Friedemann et al, ) properties. Coptidis rhizoma is known to harbor a diversity of active isoquinoline‐type alkaloids such as berberine, coptisine, epiberberine, palmatine and jatrorrhizine.…”

UPLC/Q-TOFMS-Based Metabolomics Approach to Reveal the Protective Role of Other Herbs in An-Gong-Niu-Huang Wan Against the Hepatorenal Toxicity of Cinnabar and Realgar

“…In fact, there have been numerous literature reporting several herbs in AGNH exert anti-inflammatory and hepatorenal protective effects. Cui et al demonstrated that Coptidis rhizoma could inhibit the overproduction of inflammatory meditors in macrophages induced by lipopolysaccharide, including induced nitric oxide synthase (iNOS), nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) ( Cui et al, 2014 ). Furthermore, berberine, the main bioactive ingredient of C. rhizoma , could alleviate cisplatin-induced nephrotoxicity in mice by inhibiting inflammation and oxidative/nitrosative stress.…”