<i>Continuous Recording of Blood Oxygen Tensions by Polarography</i>

Clark, Leland C.; Wolf, Richard M.; Granger, Donald L.; Taylor, Zena

doi:10.1152/jappl.1953.6.3.189

Cited by 811 publications

(305 citation statements)

References 0 publications

Supporting

Mentioning

290

Contrasting

Unclassified

Order By: Relevance

“…This dye has been used by other investigators to measure the concentration of oxygen in solutions (291, cells (4), tissues (19), and organs (21). We have found this dye-based method to be less variable than polarographic methods (6) for oxygen measurements in hypoxic yeast cultures. Other advantages of using this method as a n alternative to electrode-based methods for studies of this type are described elsewhere (19).…”

Section: Peroxide Production and Cell Viability Inmentioning

confidence: 92%

Characterization of hypoxia‐dependent peroxide production in cultures of Saccharomyces cerevisiae using flow cytometry: A model for ischemic tissue destruction

Yurkow

McKenzie

1993

Cytometry

View full text Add to dashboard Cite

Peroxide production in cultures of Saccharomyces cerevisiae was measured using the H202-sensitive fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA) and flow cytometry. Aeration of cultures of S. cerevisiae exposed to a period of hypoxia was found to induce elevated levels of peroxide that were 100-fold higher than the levels observed in cultures maintained under exclusively aerated or hypoxic conditions. Simultaneous viability analysis, using the fluorescent DNA-intercalating dye propidium iodide, indicated that the increase in peroxide generation preceded cell damage and death. Various agents were found to influence the effect of peroxides on cell viability. The addition of ethanol to hypoxic stationary cultures dramatically increased the rate of cell death without further increasing the amount of peroxide produced, while glucose inhibited peroxide production and decreased the rate of cell death. Surprisingly, elevated peroxide levels of hypoxiclreaerated cultures were maintained upon addition of KH2P0,, although the cells remained viable for extended periods of time when compared to control and other test cultures. Similarities between our observations and those of other investigators using anoxidreperfused organs suggest that hypoxic/reaerated yeast cultures may be a useful model system to study ischemia-dependent tissue destruction of mammals.0 1993 Wiley-Liss, Inc.

show abstract

Section: Peroxide Production and Cell Viability Inmentioning

confidence: 92%

Characterization of hypoxia‐dependent peroxide production in cultures of Saccharomyces cerevisiae using flow cytometry: A model for ischemic tissue destruction

Yurkow

McKenzie

1993

Cytometry

View full text Add to dashboard Cite

show abstract

“…Respiratory rates were monitored with Clark oxygen electrodes (23) . Mitochondria gave ADP/O ratios of two for succinate and acceptor control ratios of 5 to 7 .…”

Section: Methodsmentioning

confidence: 99%

Energy-Linked Ultrastructural Transformations in Isolated Liver Mitochondria and Mitoplasts

Hackenbrock

1972

The Journal of Cell Biology

View full text Add to dashboard Cite

An investigation was carried out in which microsamples of isolated rat liver mitochondria and freshly prepared mitoplasts in defined energy states were freeze-cleaved . Parallel microsamples were fixed with osmium tetroxide and with glutaraldehyde followed by osmium tetroxide as previously used in this laboratory for the preservation of energy-linked mitochondrial configurations . The details of the orthodox configuration of energized mitochondria and the condensed configuration of de-energized mitochondria, as revealed previously by chemical fixation, are confirmed in this report for nonfixed, freeze-cleaved mitochondria . The precise agreement in preservation of configuration obtained by the physical fixation of rapid freezing and by chemical fixation establishes unequivocally that mitochondria undergo energy-linked ultrastructural transformation between the condensed and the orthodox configurations which are thus natural structural states related to the metabolic activity of the mitochondrion . Configurations observed by freeze-cleaving and by chemical fixation reveal that mitoplasts also undergo a specific and dramatic ultrastructural transformation with the induction of oxidative phosphorylation . The transformation appears to be isovolumetric and therefore is thought to be mediated through energized conformational activity in the surface electron-transport membrane of the mitoplast . Passively swollen, spherical, osmotically active mitoplasts could not be fixed rapidly enough by chemical fixatives as normally used without altering the spherical form . In this special case preservation of configurational form required rapid freezing or chemical fixatives of low osmolar concentration . 450

show abstract

“…The tip of a Clark oxygen electrode (17) was located in the photometer cuvette just above the light path . Pyridine nucleotide was excited at 366 nM and the resulting fluorescence emission, a measure of oxidation-reduction level, was selected at 440-460 nM at 270° (15,18) .…”

Section: Methodsmentioning

confidence: 99%

Oxidative Phosphorylation and Ultrastructural Transformation in Mitochondria in the Intact Ascites Tumor Cell

Hackenbrock

Rehn

Weinbach

et al. 1971

The Journal of Cell Biology

164

View full text Add to dashboard Cite

We have examined the ultrastructure of mitochondria as it relates to energy metabolism in the intact cell . Oxidative phosphorylation was induced in ultrastructurally intact Ehrlich ascites tumor cells by rapidly generating intracellular adenosine diphosphate from endogenous adenosine triphosphate by the addition of 2-deoxyglucose . The occurrence of oxidative phosphorylation was ascertained indirectly by continuous and synchronous monitoring of respiratory rate, fluorescence of pyridine nucleotide, and 90° light-scattering . Oxidative phosphorylation was confirmed by direct enzymatic analysis of intracellular adenine nucleotides and by determination of intracellular inorganic orthophosphate . Microsamples of cells rapidly fixed for electron microscopy revealed that, in addition to oxidative phosphorylation, an orthodox -> condensed ultrastructural transformation occurred in the mitochondria of all cells in less than 6 sec after the generation of adenosine diphosphate by 2-deoxyglucose . A 90° light-scattering increase, which also occurs at this time, showed a t 12 of only 25 sec which agreed temporally with a slower orthodox ---> maximally condensed mitochondrial transformation . Neither oxidative phosphorylation nor ultrastructural transformation could be initiated in mitochondria in intact cells by the intracellular generation of adenosine diphosphate in the presence of uncouplers of oxidative phosphorylation . Partial and complete inhibition of oxidative phosphorylation by oligomycin resulted in a positive relationship to partial and complete inhibition of 2-deoxyglucose-induced ultrastructural transformation in the mitochondria in these cells . The data presented reveal that an orthodox -* condensed ultrastructural transformation is linked to induced oxidative phosphorylation in mitochondria in the intact ascites tumor cell.

show abstract

Continuous Recording of Blood Oxygen Tensions by Polarography

Cited by 811 publications

References 0 publications

Characterization of hypoxia‐dependent peroxide production in cultures of Saccharomyces cerevisiae using flow cytometry: A model for ischemic tissue destruction

Characterization of hypoxia‐dependent peroxide production in cultures of Saccharomyces cerevisiae using flow cytometry: A model for ischemic tissue destruction

Energy-Linked Ultrastructural Transformations in Isolated Liver Mitochondria and Mitoplasts

Oxidative Phosphorylation and Ultrastructural Transformation in Mitochondria in the Intact Ascites Tumor Cell

Contact Info

Product

Resources

About