Oxidative Phosphorylation and Ultrastructural Transformation in Mitochondria in the Intact Ascites Tumor Cell

Hackenbrock, Charles R.; Rehn, Terry G.; Weinbach, Eugene C.; Lemasters, John J.

doi:10.1083/jcb.51.1.123

Cited by 164 publications

(76 citation statements)

References 18 publications

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“…33,34 In the apoptotic cells we found two subsets of mitochondria, and in addition the respiring mitochondria display now the conformation related to increased ATP generation. Hackenbrock called this the`condensed conformation', but also stresses that a more specific sign of stimulated mitochondria is the intracristal dilatation.…”

Section: Discussionmentioning

confidence: 96%

“…Hackenbrock called this the`condensed conformation', but also stresses that a more specific sign of stimulated mitochondria is the intracristal dilatation. 34 In fact a previous study on etoposide treated THP.1 cells showed intracristal dilatation in the`ultracondensed' mitochondria. 17 Moreover, besides the altered mitochondria, these authors reported casually the persistence of mitochondria with normal ultrastructural configuration.…”

Section: Discussionmentioning

confidence: 99%

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Ultrastructural localization of cytochrome c in apoptosis demonstrates mitochondrial heterogeneity

et al. 2000

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Release of apoptogenic factors into the cytosol including cytochrome c is triggering the execution phase of apoptosis through activation of cytoplasmic effector caspases. How loss of function of the electron transport chain can be reconciled with an adequate energy supply necessary for executing the apoptotic program was studied in granulosa cell (GC) sheets cultured up to 72 h without gonadotrophic support. Cytochrome c was localized ultrastructurally by oxidation of diaminobenzidine tetrahydrochloride both in living and fixed cells. In uncultured GC sheets all cells show staining over their entire mitochondrial population. In 72 h cultured sheets in the absence of FSH pre-apoptotic GC's display two subsets of mitochondria: normal sized stained mitochondria and small orthodox mitochondria without demonstrable cytochrome function. Apoptotic cells contain several mitochondria with preservation of respiratory function besides unstained orthodox mitochondria. The cytochrome c containing mitochondria typically display dilated intracristal spaces, a mitochondrial conformation related to increased ATP production. Cytochrome c release was confirmed by Western blotting. In 72 h cultures supplemented with FSH, GC's displayed staining over their entire mitochondrial population. In cultures lacking FSH, but partially protected from apoptosis through caspase inhibition, the cytochrome c release was not inhibited. Thus in the present studied model dysfunction of only a subset of mitochondria is instrumental to initiate the apoptotic program while a functional electron transport chain is maintained until the degradation phase in a subset of respiring mitochondria. Cell Death and Differentiation (2000) 7, 331 ± 337.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Ultrastructural localization of cytochrome c in apoptosis demonstrates mitochondrial heterogeneity

et al. 2000

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“…The signi®cance of this mitochondrial morphological transformation remains to be elucidated. However, condensed mitochondria have been associated with higher oxidative phosphorylation e ciency (De Martino et al, 1979;Hackenbrock et al, 1971). Therefore, it is possible that mitochondrial transformation might be a cellular mechanism to compensate for decreased oxygen supply to cells in the adluminal compartment due to the blood-testis barrier (Waites and Gladwell, 1982).…”

Section: Discussionmentioning

confidence: 99%

SPAF, a new AAA-protein specific to early spermatogenesis and malignant conversion

et al. 2000

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“…In vivo mitochondrial contractions were obtained by treating 1-mm thick sections of corn shoots with 2.5 mM 2DOG2 for 20 min (11). The sections were then fixed in 2% glutaraldehyde in 0.2 M cacodylate buffer.…”

Section: Methodsmentioning

confidence: 99%

Corn Mitochondrial Swelling and Contraction—an Alternate Interpretation

Malone¹,

Koeppe²,

Miller³

1974

Plant Physiol.

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Mitochondria isolated from 3-day-old etiolated corn shoots (Zea mays L.) can be categorized into three separate groups, each group characteristic of the cell type from which the mitochondria were isolated. Phloem sieve tubes and some adjacent parenchyma cells contain (12,24,26). Swelling involves the unfolding of the inner membrane and a decrease in the amorphous matrix, while contraction involves the infolding of the inner membrane and increase in matrix density. The presence of contracted, partially swollen, and swollen mitochondria in experimental in vitro preparations that should contain only contracted (or swollen) mitochondria is believed to result from mitochondria responding at different rates (26). A ready reversibility has been reported to exist between swollen, partially swollen, and contracted mitochondria (24).In vivo differences in the number of cristae and amount of amorphous matrix in different cell types for several species of plants have been reported (5, 7-9, 13, 16, 19, 22). Chrispeels et al. (6) Isolated mitochondria were fixed either while they were suspended or after being pelleted at 12,000 rpm for 10 min. Fixatives for isolated mitochondria or intact shoots were 1 or 2% glutaraldehyde either in 0.1 M phosphate buffer or in 0.2 M cacodylate buffer (pH 7.4) followed by 1 % OsO in the respective buffer; or only 1 % OSO4 in 0.1 M phosphate buffer or 0.2 M cacodylate buffer. Dehydration was in a graded ethyl alcohol series, and propylene oxide was used as a solvent for the Epon embedment (15).In vivo mitochondrial contractions were obtained by treating 1-mm thick sections of corn shoots with 2.5 mM 2DOG2 for 20 min (11). The sections were then fixed in 2% glutaraldehyde in 0.2 M cacodylate buffer.One-,um thick plastic sections of whole plant material were cut and observed with phase contrast optics for orientation purposes. Subsequently, thin plastic sections were cut from the adjacent area of the block. All thin sections were observed on an RCA EMU4 or Siemens Elmiskop I electron microscope after staining in uranyl acetate (23) and lead citrate (21).Areas of I. C. and 0. C. of type III mitochondria were determined by superimposing a dot grid on a micrograph and counting dots falling in the compartments. RESULTSMitochondrial profiles fell into three readily recognizable types and were classified according to the type which they most

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Oxidative Phosphorylation and Ultrastructural Transformation in Mitochondria in the Intact Ascites Tumor Cell

Cited by 164 publications

References 18 publications

Ultrastructural localization of cytochrome c in apoptosis demonstrates mitochondrial heterogeneity

Ultrastructural localization of cytochrome c in apoptosis demonstrates mitochondrial heterogeneity

SPAF, a new AAA-protein specific to early spermatogenesis and malignant conversion

Corn Mitochondrial Swelling and Contraction—an Alternate Interpretation

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