Comamonas sp. 3ah48 is a dibenz[ a,h ]anthracene‐degrading bacterium that is tolerant to heavy metals

Abstract

Industrialization often causes polycyclic aromatic hydrocarbon (PAH) and heavy metal contamination of soil and water. In this study, we isolated a bacterium from bottom mud water around a park of Kawasaki Port, Japan, that degrades the 5‐ring PAH dibenz[a,h]anthracene (DBA). The strain, Comamonas sp. 3ah48, degraded 29% of DBA (30 μg ml−1) in 7 days, and the degradation level increased drastically, to 59%, by the addition of glutamate to the medium. The strain also degraded 40, 14, 15 and 19% of pyrene (Pyr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF) and benzo[g,h,i]perylene (BghiP) respectively. Benzo[a]pyrene (BaP) was degraded only when glutamate was added to the medium. Strain 3ah48 retained its degradation levels in the presence of 2 mmol l−1 Co2+, Zn2+ or Cr2+, at almost the same level as that without metal, and increased the DBA degradation level to 57% in the presence of 2 mmol l−1 Cu2+, suggesting the possibility of the presence of laccase. Significance and Impact of the Study Sixteen polycyclic aromatic hydrocarbons (PAHs) are listed as priority pollutants by the United States Environmental Protection Agency (USEPA). Information about the biodegradation of one of those PAHs, dibenz[a,h]anthracene (DBA), is limited. The present study focuses on DBA degradation by Comamonas sp. 3ah48 strain isolated around Kawasaki Port, Japan. Comamonas sp. 3ah48, cultured with the addition of glutamate to the medium, was found to increase the degradation level of DBA and to degrade DBA even in the presence of high concentrations of heavy metals.

How do microbial communities deal with chronic hydrocarbon presence in oil seep soils? Data from historical hand‐dug oil wells

“…In addition to the aforementioned isolated degraders, strains of the Williamsia , Rhodococcus , and Comamonas genera also comprised numerous groups with metabolic profiles, including those pertaining to crude oil, n C16, and phenol (Table S2). These observations are in accordance with the data provided by other authors (Dahal et al, 2017; Obuekwe et al, 2009; Okai et al, 2019; Yassin et al, 2007; Yuan et al, 2020). Taking into account all these findings, the dominant degraders are abundant in seep and HC‐impacted sites, and may be responsible for in situ hydrocarbon removal.…”

A Multi-Omics Study of Familial Lung Cancer: Microbiome and Host Gene Expression Patterns

“…On the other hand, some genera decreased or depleted in cancer tissue, presenting a different trend: we found Comamonas often decreased in cancer; while species of Comamonas could cause opportunistic infection but are also known to degrade PAHs and are tolerant to heavy metals ( 42 , 43 ). Additionally, Peptococcus were not detected in cancer tissue but only found in normal tissue; but previous work suggested Peptococcus is also an opportunistic pathogen ( 44 ).…”

“…The 2nd included: Lactobacillus, Blautia, Oscillospira, which were recognized or predicted future probiotics, supposed to play a positive role in the microbiome ( 56 , 65 ). Notably, the third involved: Sphingomonas, Sphingopyxis, Novosphingobium, Comamonas, Rhodococcus, Thermomonas, Bradyrhizobium, Kaistobacter, and Cloacibacterium, which are well-known for their degradation and detoxication of PAHs, pesticide, heavy metals and organometallic compounds ( 38 – 43 , 47 , 49 , 50 , 61 , 63 , 64 , 66 ). Importantly, these microbes were frequently enriched in subjects of the FLC and IAP groups.…”

Evaluation of Amycolaptosis sp. Ver12 as a potential degrader of benzo(b)fluoranthene

“…3ah48 was able to degrade 14% of BbF from an initial concentration of 0⋅03 mg l −1 in 7 days and when glutamate (2 mmol l −1 ) was added as a cometabolite, it reached a degradation level of 18% (Okai et al . 2019).…”