Clostridium butyricumas probiotic for promoting growth performance, feed utilization, gut health and microbiota community of tilapia (Oreochromis niloticus × O. aureus)

Abstract

The feeding trial was conducted to evaluate the potentials of Clostridium butyricum in the diet of tilapia. Fish (~14 g) were fed with basal diet supplemented with 0 (Control), 0.5 (C‐1), 1 (C‐2), 2 (C‐3), 4 (C‐4) and 8 (C‐5) g/kg commercial probiotic‐containing C. butyricum (1.5 × 108 CFU/g) for 8 weeks. The results showed that weight gain significantly increased, and feed conversion ratio decreased in the C‐2, C‐3 and C‐4 groups (p < .05). The protein retention (except C‐1 group), lipid retention and apparent digestibility coefficient (ADC) of dry matter in probiotic supplementation groups were significantly enhanced, and ADC of protein in the C‐4 group was also improved (p < .05). The supplementation of probiotic significantly increased villus height in anterior intestines and reduced the numbers of intestinal Escherichia coli (p < .05). High‐throughput sequencing showed that top three phyla namely Planctomycetes in all probiotic‐containing groups, Proteobacteria in the C‐1 and C‐2 groups and Chloroflexi in the C‐3 group had higher level than the NC group. The cumulative mortality was reduced by dietary probiotic after challenging with Aeromonas hydrophila (p < .05). In conclusion, C. butyricum can be supplemented at 1–2 g/kg feed for promoting the growth, feed utilization, gut health and microbiota of tilapia.

Clostridium butyricum: a promising probiotic confers positive health benefits in aquatic animals

“…2019c; Poolsawat et al . 2020). In M. miiuy fed C. butyricum (at different doses of 10 3 –10 9 cfu g −1 ), an increase in growth performance (expressed as the total weight gain, relative weight gain, feed utilisation efficiency and specific growth rate) and a decrease in the feed conversion ratio (FCR) were associated with an increase in the amount of C. butyricum and the optimal dose was 10 9 cfu g −1 , when compared with the controls (Song et al .…”

“…Additionally, Poolsawat et al . (2020) fed tilapia ( O. niloticus × O. aureus ) with a diet supplemented with C. butyricum [at 0, 0.5, 1, 2, 4 or 8 g kg −1 commercial probiotic‐containing C. butyricum (1.5 × 10 8 cfu g −1 )] for 8 weeks, and revealed an enhancement in the weight gain, protein retention, lipid retention and apparent digestibility coefficient of dry matter, as well as a decrease of feed conversion ratio.…”

“…In a study with tilapia ( O. niloticus × O. aureus ), C. butyricum was administered for 8 weeks and increased haemoglobin level and activities of lysozyme, glutamic pyruvic transaminase, catalase, glutamic oxaloacetic transaminase, and SOD were observed (Poolsawat et al . 2020).…”

“…In addition, tilapia ( O. niloticus × O. aureus ) fed diets supplemented with C. butyricum for 8 weeks significantly improved survival rate compared control group after challenged with A. hydrophila (Poolsawat et al . 2020).…”

The potentials of fructooligosaccharide on growth, feed utilization, immune and antioxidant parameters, microbial community and disease resistance of tilapia (Oreochromis niloticus × O. aureus)

“…All media were purchased from Qingdao Hope Bio-Technology Co., Ltd.. The microbial study using cultivation media followed the method described by Poolsawat, Li, He, Dong, and Leng (2020).…”