<i>Chlamydia pneumoniae</i>DNA Detection in Peripheral Blood Mononuclear Cells Is Predictive of Vascular Infection

Blasi, Francesco; Boman, Jens; Esposito, Gloria; Melissano, Germano; Chiesa, Roberto; Cosentini, Roberto; Tarsia, Paolo; Tshomba, Yamume; Betti, Monia; Alessi, Marie‐Christine; Morelli, Nicoletta; Allegra, L.

doi:10.1086/315126

Cited by 100 publications

(104 citation statements)

References 10 publications

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“…Previous studies reported different prevalence of CP by PCR in specimens obtained from abdominal aortic aneurysm, ranging from 20% to 49%. 4,5,6,7 Therefore our positivity rates are within the expected range. In our study, an optimized DNA extraction protocol to reduce any underestimation of CP prevalence was used, and our data were confirmed by nonradioactive DNA hybridization to enhance specificity.…”

Section: Discussionsupporting

confidence: 74%

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

et al. 2002

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Background-Only a few studies have focused the attention on the relation between elevated anti-Chlamydia pneumoniae (CP) antibodies and the detection of CP in the arterial wall. The aim of our study is thus to investigate the relationship between immune response to CP and detection of CP in the aortic walls of patients with abdominal aortic aneurysm. Methods and Results-A specimen of aortic wall was obtained from 102 consecutive patients who underwent abdominal aneurysm repair. The possible presence of CP was studied by polymerase chain reaction and confirmed by nonradioactive DNA hybridization. Antibody response to CP was studied (IgG, IgA titers). We found 33 patients (32.4%) with CP DNAϩ. No correlation between CP DNA detection and antibody titers was found (IgG Pϭ0.52, IgA Pϭ0.66). High correlation between IgG and IgA titer was observed (PϽ0.01). Endovascular presence of CP and antibody titers was not related to the age of the patient. Conclusions-CP antibody titers are not associated with the presence of CP in the aortic wall of patients with abdominal aortic aneurysm.

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Section: Discussionsupporting

confidence: 74%

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

et al. 2002

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“…Nasopharyngeal aspirate samples in 2 mL of transport medium containing a sucrose phosphate buffer were frozen at -708C. Nested polymerase chain reaction (PCR) was performed for both pathogens with validated methods, as previously described [17,18]. To avoid the risk of contamination, sample preparation, PCR amplification and product analysis were performed in separate rooms.…”

Section: Methodsmentioning

confidence: 99%

“…Touchdown nested PCR for detection of C. pneumoniae DNA was performed using primers designed to detect the major outer membrane protein [19]. Extracted DNA solution (10 mL in a total volume of 50 mL) was used in the first PCR round; 5 mL of the PCR products amplified by the outer primers was then transferred to a new 50-mL PCR reaction mix for a second amplification using the inner primers [18]. The first round consisted of 40 cycles and the second 35.…”

Section: Methodsmentioning

confidence: 99%

Importance of acute Mycoplasma pneumoniae and Chlamydia pneumoniae infections in children with wheezing

Esposito¹,

Blasi²,

Arosio³

et al. 2000

Eur Respir J

179

130

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In order to evaluate the role of Mycoplasma pneumoniae and Chlamydia pneumoniae in reactive airway disease, 71 children aged 2±14 yrs with an acute episode of wheezing and 80 age-matched healthy children were studied.Sera for the determination of specific antibody levels and nasopharyngeal aspirates for the detection of M. pneumoniae and C. pneumoniae deoxyribonucleic acid were obtained on admission and after 4±6 weeks. All children with wheezing received a standard therapy with inhaled corticosteroids and bronchodilators for 5±7 days; when antibiotic was added on the basis of the judgement of the paediatrician in charge, clarithromycin 15 mg . kg body weight -1 . day -1 for 10 days was used.Acute M. pneumoniae and C. pneumoniae infections were detected significantly more often in children with wheezing than in controls. In patients infected with one of the two pathogens, a history of recurrent wheezing was significantly more frequent than in those without either infection. During a 3-month follow-up period, among nonantibiotic-treated children, those with acute M. pneumoniae and/or C. pneumoniae infection showed a significantly higher recurrence of wheezing than those without acute M. pneumoniae and/or C. pneumoniae infection (p=0.03).These results highlight the apparently significant relationship of Mycoplasma pneumoniae and Chlamydia pneumoniae with wheezing in children, particularly in subjects with a history of recurrent episodes, and the possible improvement in the course of reactive airway disease within paediatric patients with acute Mycoplasma pneumoniae and/or Chlamydia pneumoniae infection.

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“…Nasopharyngeal aspirates were also obtained, immediately immersed in sucrose-phosphateglutamate transport medium, and stored at x70uC until assayed for the presence of M. pneumoniae and C. pneumoniae deoxyribonucleic acid (DNA). PCR for both pathogens was performed as previously described [16,17]. Chest radiographs (erect posteroanterior and lateral view) were taken in the hospital of admission and were then centrally reviewed by an experienced radiologist who was naive to the patients' clinical history or laboratory data.…”

Section: Methodsmentioning

confidence: 99%

Mycoplasma pneumoniaeandChlamydia pneumoniaeinfections in children with pneumonia

Esposito¹,

Blasi²,

Bellini³

et al. 2001

Eur Respir J

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The most common clinical signs, host responses and radiographic patterns were studied in 203 Italian children hospitalized for community-acquired pneumonia in order to clarify the role of clinical and radiological characteristics in the diagnosis of Mycoplasma pneumoniae and/or Chlamydia pneumoniae infections.Antibody measurements in paired sera and polymerase chain reaction on nasopharyngeal aspirates were used to establish the diagnoses of acute M. pneumoniae and C. pneumoniae infection, and the aetiologic data were correlated with the clinical, laboratory and radiographic data obtained on admission.No significant association was observed between evidence of M. pneumoniae and/or C. pneumoniae infection and periods of episode during the year, mean age of the study subjects, individual symptoms, physical findings or laboratory test results. Furthermore, no significant correlation was observed in relation to the radiological findings and M. pneumoniae and/or C. pneumoniae infection.This study shows that neither clinical findings nor laboratory parameters distinguished Mycoplasma pneumoniae and/or Chlamydia pneumoniae infection in children with pneumonia. Radiological findings also have a limited capacity to differentiate aetiologic agents. The priorities for future research include the development of rapid, easily accessible and cost-effective diagnostic tests useful for each episode of pneumonia in children.

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Chlamydia pneumoniaeDNA Detection in Peripheral Blood Mononuclear Cells Is Predictive of Vascular Infection

Cited by 100 publications

References 10 publications

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

Lack of Association Between Serum Immunoreactivity and Chlamydia pneumoniae Detection in the Human Aortic Wall

Importance of acute Mycoplasma pneumoniae and Chlamydia pneumoniae infections in children with wheezing

Mycoplasma pneumoniaeandChlamydia pneumoniaeinfections in children with pneumonia

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