2017
DOI: 10.17660/actahortic.2017.1155.4
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Castanea spp. hybrid clones in vitro conservation: synthetic seeds vs. slow growth storage

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Cited by 5 publications
(2 citation statements)
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“…One of the advantages of tissue culture is the capacity to preserve germplasm in vitro for long periods without the large investments in land, labour, water, fertiliser, and pesticide that would be required for plantation-or nursery-based germplasm archives [42,[51][52][53]178,179]. In vitro storage can also delay the maturation of valuable clones, especially if their shoots or callus are stored under minimal-growth or nil-growth conditions [39,40,42,[180][181][182].…”
Section: In Vitro Preservationmentioning
confidence: 99%
“…One of the advantages of tissue culture is the capacity to preserve germplasm in vitro for long periods without the large investments in land, labour, water, fertiliser, and pesticide that would be required for plantation-or nursery-based germplasm archives [42,[51][52][53]178,179]. In vitro storage can also delay the maturation of valuable clones, especially if their shoots or callus are stored under minimal-growth or nil-growth conditions [39,40,42,[180][181][182].…”
Section: In Vitro Preservationmentioning
confidence: 99%
“…Better results were obtained by Capuana and Di Lonardo [21] in the storage of shoot cultures of C. sativa; they achieved survival percentages greater than 80% after 48 months at 8 • C. A storage temperature of 4 • C significantly decreases the survival percentages, only recovering 56% of the cultures after 12 months storage, and 0% survival after 24 months. Gomes et al [97] compared two techniques (synthetic seeds and slow growth storage) for the preservation of hybrid clones produced from controlled crosses between C. sativa × C. crenata and C. sativa × C. mollissima. The best treatment was achieved with synseeds stored for six months with sterile distilled water, and with slow growth storage with 0.22 M sucrose.…”
Section: Medium-term Preservation (Slow Growth)mentioning
confidence: 99%