Carpesium abrotanoides (L.) Root as a Potential Source of Natural Anticancer Compounds: Targeting Glucose Metabolism and PKM2/HIF‐1α Axis of Breast Cancer Cells

Abstract

Carpesium abrotanoides L. (CA) is widely used as a medicinal plant in Asia. The biological activities of the extract from the roots of Carpesium abrotanoides L. (PCA) and its major components were analyzed in this study. PCA was separated and identified with mass spectrometry. Furthermore, we sought to elucidate the anticancer activity of PCA and its mechanisms. PCA exerted its anti-breast cancer activity through inhibiting the expression of glycolysis-related genes, such as glucose transporter 1, lactate dehydrogenase A, and hexokinase 2. Moreover, PCA downregulated the expression of pyruvate kinase M2 and altered its cellular translocation. We also demonstrated PCA is an inhibitor of the PKM2/hypoxia-inducible factor-1α axis, indicating that PCA is potentially useful as an anti-breast cancer agent.Practical Application: In this study, the extract from roots of Carpesium abrotanoides Linn. (PCA) was shown to have a noticeable anticancer effect against breast cancer in vitro, and PCA exerts the anticancer activity by regulating glucose metabolism and PKM2 expression. These findings indicate that PCA is a promising agent with practical applications in the development of functional food containing Carpesium abrotanoides L. root extracts.

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

“…Besides, transwell assay indicated that the abilities of migration and invasion were markedly inhibited in glioma cells treated with Sev ( Figure 1F and G). As glucose metabolism was associated with cancer progression, [21][22][23] we checked the levels of glucose and lactate in glioma cells. The data showed that the rates of glucose consumption and lactate production were gradually decreased in glioma cells with the growing concentrations of Sev ( Figure 1H-K).…”

Unravelling the Anticancer Mechanisms of Traditional Herbal Medicines with Metabolomics

“…• Carpesium abrotanoides Linnaeus. root extract reduced glycolysis by downregulating the expression of PKM2 and inhibited PKM2/HIF-1α in breast cancer cells [69].…”

Antiprostate Cancer Activity of Ineupatolide Isolated from Carpesium cernuum L.

“…Previous studies have shown that Carpesium cernuum L. contains a variety of chemical components, some of which exhibit different antitumor, anti-inflammatory, antioxidant, and immunomodulatory effects [ 11 – 13 ]. In addition, it has been reported that the primary chemical components of Carpesium cernuum L. are terpenoids and flavonoids [ 14 ].…”