<i>Candida</i> species detection in patients with chronic periodontitis: A systematic review and meta‐analysis

Slazhneva, E. S.; Tikhomirova, E.A.; Царев, В.Н.; Iordanishvili, A. K.; Лобода, Е. С.; Atrushkevich, V. G.

doi:10.1002/cre2.635

Cited by 12 publications

(12 citation statements)

References 58 publications

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“…This finding is consistent with a systematic review, which showed that Candida spp. may increase the risk of periodontal disease development by 1.76 times [ 47 ]. Candida albicans can interact with periodontopathic pathogens to develop mixed biofilms, suggesting that fungi may play an active role in the inflammatory destructive process during the onset and progression of periodontal diseases [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Characterization and Comparative Analysis of Removable Denture-Wearing and Non-Denture-Wearing Individuals in Healthy and Diseased Periodontal Conditions

Wong,

Hung,

Yip

et al. 2024

Microorganisms

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Removable denture wearers are at an increased risk of developing periodontal diseases due to biofilm deposition and microbial colonization on the denture surface. This study aimed to characterize and compare the metagenomic composition of saliva in denture wearers with different periodontal statuses. Twenty-four community-dwelling elders were recruited and grouped into denture wearers with active periodontitis (APD), non-denture wearers with active periodontitis (APXD), denture wearers with stable periodontal health conditions (SPCD), and non-denture wearers with stable periodontal health conditions (SPCXD). Saliva samples were collected and underwent Type IIB restriction-site-associated DNA for microbiome (2bRAD-M) metagenomic sequencing to characterize the species-resolved microbial composition. Alpha diversity analysis based on the Shannon index revealed no significant difference between groups. Beta diversity analysis using the Jaccard distance matrix was nearly significantly different between denture-wearing and non-denture-wearing groups (p = 0.075). Some respiratory pathogens, including Streptococcus agalactiae and Streptococcus pneumoniae, were detected as the top 30 species in saliva samples. Additionally, LEfSe analysis revealed a substantial presence of pathogenic bacteria in denture groups. In the cohort of saliva samples collected from community-dwelling elders, a remarkable abundance of certain opportunistic pathogens was detected in the microbial community.

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Section: Discussionmentioning

confidence: 99%

Metagenomic Characterization and Comparative Analysis of Removable Denture-Wearing and Non-Denture-Wearing Individuals in Healthy and Diseased Periodontal Conditions

Wong,

Hung,

Yip

et al. 2024

Microorganisms

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“…One aspect we should acknowledge also as a limitation is the lack of information regarding the periodontal health of the patients, which might have had an impact on the colonization of Candida sp. When the close association between C. albicans and chronic periodontitis is taken into consideration [72,73], the presence of periodontitis and poor oral health might alter the abundance of C. albicans and, due to morphogenetic similarity, of C. tropicalis in mild/moderate dysplasia, carcinoma in situ, and OSCC.…”

Section: Discussionmentioning

confidence: 99%

Real-Time PCR Detection of Candida Species in Biopsy Samples from Non-Smokers with Oral Dysplasia and Oral Squamous Cell Cancer: A Retrospective Archive Study

İlhan,

Vural,

Gürhan

et al. 2023

Cancers

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The impact of Candida sp. in the development of oral cancer remains uncertain and requires sensitive analytical approaches for clarification. Given the invasive capabilities of these microorganisms in penetrating and invading host tissues through hyphal invasion, this study sought to detect the presence of five Candida sp. in oral biopsy tissue samples from non-smoker patients. Samples were obtained from patients at varying stages of oral carcinogenesis, including dysplasia, carcinoma in situ, OSCC, and histologically benign lesions, and analyzed using Real-Time PCR. Oral tissue samples from 80 patients (46 males and 34 females) were included. Significantly higher C. albicans presence was detected in the mild/moderate dysplasia group compared to the healthy (p = 0.001), carcinoma in situ (p = 0.031) and OSCC groups (p = 0.000). Similarly, C. tropicalis carriage was higher in tissues with mild/moderate dysplasia compared to healthy (p = 0.004) and carcinoma in situ (p = 0.019). Our results showed a significant increase in the presence of C. albicans and C. tropicalis within the mild/moderate dysplasia group compared to other cohorts. Coexistence of these two microorganisms was observed, suggesting a potential transition from a commensal state to an opportunistic pathogen, which could be particularly linked to the onset of oral neoplasia.

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“…Во всех образцах преобладали представили таксономической группы Streptococcus. У обеих групп распространенность таксономической группы Streptococcus составила 23,86% (I группа) и 22,91% (II группа), что в среднем ниже нормального уровня Streptococcus и может косвенно свидетельствовать о снижении иммунного статуса [ 1 ] .…”

Section: терапияunclassified

“…Помимо пародонтальных патогенов, зубной налет содержит другие бактерии, а также грибы, роль которых активно изучается. Многие исследователи считают, что дрожжеподобные грибы, в частности Candida spp., являются одной из важных причин развития, прогрессирования и осложненного течения ХГП [ 1 ] -они условно-патогенны, колонизируют слизистую оболочку рта и кожу здоровых людей, а также считаются предупредительным признаком иммунного расстройства. Этот условно-патогенный микроорганизм длительное время может персистировать в разных нишах рта без клинических проявлений, однако при снижении иммунного статуса (например, у пациентов с сахарным диабетом или иммунодефицитом, у детей раннего возраста или у пожилых людей) или при изменении окружающей среды его факторы вирулентности могут вызывать заболевание.…”

Section: Introductionunclassified

Features of the oral microbiome in the association of periodontitis and candidiasis in the postcovid period

Цинеккер,

Модина,

Хусаинов

et al. 2023

Clinical Dentistry (Russia)

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Совершенствование диагностики и оказания стоматологической помощи пациентам с хроническим пародонтитом в сочетании с кандидозным стоматитом — актуальная цель многих исследований. Пандемия COVID-19, в том числе течение ее постковидного периода, несколько изменила взгляд специалистов на диагностику и лечение указанных заболеваний. Цель исследования — метагеномный анализ микробиоты пациентов с пародонтитом в сочетании с кандидозным стоматитом в постковидном периоде. В экспериментальную I группу вошли 12 пациентов в постковидном периоде, не вакцинированные, с диагнозом «кандидозный стоматит» (B37.0); во II группу (сравнения) — 11 пациентов, не инфицированных новой коронавирусной инфекцией, не вакцинированных (сбор материала проведен до пандемии COVID-19). Отбор биоматериала произведен из пародонтальных карманов после профессиональной гигиены рта. Культуральные и морфологические свойства грибов рода Candida изучены классическим способом, идентификация выполнена с помощью MALDI-TOF MS, метагеномный анализ — с использованием спектрофотометра Nanodrop ND-2000 (Wilmington), наборов для анализа Quant-iT dsDNA HS и секвенатора MiSeq (Illumina). Выявлены 117 филотипов на уровне родов, относящихся к 21 типам, выделены 34 наиболее многочисленных филотипа на уровне родов. У пациентов I группы при идентификации грибов рода Candida определены гарантированная идентификация рода и видовые штаммы Candida albicans DSM 11945 DSM (2,106), Candida albicans CBS 1905 NT CBS (2,037), Candida albicans DSM 3454 DSM (2,011), Candida inconspicua CBS 180T CBS (1,71); а также увеличенный средний индекс биораспределения Шеннона (6,26). При распределении полученных результатов на известные микробные пародонтальные комплексы выявлено сниженное количество относительного обилия пародонтопатогенов 1-го и 2-го порядка (в 3 и 1,38 раза соответственно) на фоне увеличения микроорганизмов — комменсаллов (Actynomyces, Corynebacterium, Leptotrichia) (в 2,9 раз), что может говорить о каскаде воспалительной реакции и смене микрофлоры рта на фоне снижения местного или общего иммунитета у пациента. Выявлены особенности микробиома пародонтального кармана у пациентов с пародонтитом и кандидозом в постковидный период, идентифицированы отдельные представители микробиома, описаны их особенности, что дает основания к применению различных методов лечения, в том числе с использованием малоинвазивной терапии, на фоне антимикотиков. Improvement of diagnostics and provision of dental care to patients with chronic periodontitis combined with candidal stomatitis is an actual goal of many studies. The COVID-19 pandemic, including the course of its post-candida period, has somewhat changed the view of specialists on the diagnosis and treatment of these diseases. The aim of study is metagenomic analysis of microbiota of patients with periodontitis combined with candidal stomatitis in the post-ovoid period. . The experimental group I included 12 patients in the post-ovarian period, unvaccinated, diagnosed with candidal stomatitis (B37.0); group II (comparison) included 11 patients not infected with a new coronavirus infection, unvaccinated (material collection was performed before the COVID-19 pandemic). Biomaterial was sampled from periodontal pockets after professional oral hygiene. Culture and morphological properties of fungi of the genus Candida were studied in the classical way, identification was performed using MALDI-TOF MS, metagenomic analysis was performed using Nanodrop ND-2000 spectrophotometer (Wilmington), Quant-iT dsDNA HS analysis kits and MiSeq sequencer (Illumina). . A total of 117 phylotypes at the genus level belonging to 21 types were identified, and the 34 most abundant phylotypes at the genus level were isolated. In group I patients, the identification of fungi of the genus Candida determined guaranteed genus identification and species strains of Candida albicans DSM 11945 DSM (2.106), Candida albicans CBS 1905 NT CBS (2.037), Candida albicans DSM 3454 DSM (2.011), Candida inconspicua CBS 180T CBS (1.71); and an increased mean Shannon biodistribution index (6.26). When distributing the obtained results to the known microbial periodontal complexes, a reduced number of relative abundance of periodontopathogens of the 1st and 2nd order (3 and 1.38 times, respectively) against the background of an increase in microorganisms-commensals (Actynomyces, Corynebacterium, Leptotrichia) (2.9 times) was revealed, which may indicate a cascade of inflammatory response and change of oral microflora against the background of decreased local or general immunity in the patient. . The peculiarities of periodontal pocket microbiome in patients with periodontitis and candidiasis in the postcovial period have been revealed, individual representatives of the microbiome have been identified, their features have been described, which gives grounds for the application of various methods of treatment, including minimally invasive therapy, against the background of antimycotics.

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Candida species detection in patients with chronic periodontitis: A systematic review and meta‐analysis

Cited by 12 publications

References 58 publications

Metagenomic Characterization and Comparative Analysis of Removable Denture-Wearing and Non-Denture-Wearing Individuals in Healthy and Diseased Periodontal Conditions

Metagenomic Characterization and Comparative Analysis of Removable Denture-Wearing and Non-Denture-Wearing Individuals in Healthy and Diseased Periodontal Conditions

Real-Time PCR Detection of Candida Species in Biopsy Samples from Non-Smokers with Oral Dysplasia and Oral Squamous Cell Cancer: A Retrospective Archive Study

Features of the oral microbiome in the association of periodontitis and candidiasis in the postcovid period

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