<i>Campylobacter</i>

Vandamme, Peter; Dewhirst, Floyd E.; Paster, Bruce J.; On, Stephen L. W.

doi:10.1002/9781118960608.gbm01071

Cited by 11 publications

(10 citation statements)

References 153 publications

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“…On the other hand, different selective supplements have been described for the isolation of Campylobacter species (Vandamme et al, ). However, it remains unknown whether these supplements could also be inhibiting the growth of EHH species.…”

Section: Resultsmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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This research aims to compare the culturing conditions for enterohepatic Helicobacter, evaluating culture media, incubation atmosphere and susceptibility to antimicrobials used to generate selective conditions. Four common media for the closely related genus Campylobacter (Columbia, Bolton, Brucella and CCDA agar), as well as the need for hydrogen in the microaerobic incubation atmosphere, were evaluated. Serial dilutions of 13 strains belonging to six species (H. apodemus, H. bilis, H. canicola, H. canis, H. equorum and Helicobacter sp.) were inoculated in each media and incubated at 37°C for 48 to 96 h using CampyGen (OXOID) and gaseous exchange (including hydrogen) in parallel. Columbia or Brucella agars were the most appropriate for culturing EHH (P < 0·05). However, there was no significant difference between the atmospheres evaluated (P = 0·13). In addition, minimal inhibitory concentration for six antibiotics showed that all isolates were resistant to trimethoprim, whereas for the rest of the antibiotics (cephalothin, cefoperazone, cefsulodin, teicoplanin and vancomycin) the inhibition range was between 8 and 64 μg ml−1. Our findings suggest that Columbia or Brucella media, regardless of the use of hydrogen, can be used for the EHH isolation. In addition, the concentration of antibiotics included in commercial campylobacteria supplements is suitable for EHH species recovery. Significance and Impact of the Study Enterohepatic Helicobacter (EHH) infections have been associated with several diseases in humans such as acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases. Although they are frequently detected in clinical samples by molecular methods, only occasionally they are isolated using culture conditions described for the taxonomic related pathogen Campylobacter sp. This is because the optimal conditions for the isolation of EHH have not yet been described, which results in an underestimation of the prevalence and clinical importance of these emerging pathogens. Therefore, this study provides insight for culturing EHH species.

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Section: Resultsmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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“…H2 gas has a great impact on C. concisus growth. In laboratory cultivation, C. concisus does not grow under microaerobic conditions but has a very slow growth under anaerobic conditions [26,27] . The presence of H2 gas enables C. concisus to grow under microaerobic conditions and markedly increases its growth under anaerobic conditions [26] .…”

Section: Campylobacter Speciesmentioning

confidence: 99%

“…Whether C. ureolyticus strains isolated from the oral cavity of patients with IBD have the zot gene and the pathogenicity of C. ureolyticus Zot are currently under investigation, which may reveal a common pathogenic mechanism shared by a number of Campylobacter species. Information was obtained from [14][15][16][17][18]23,27,32,33] . Campylobacter jejuni (C. jejuni) refers to C. jejuni subsp.…”

Section: Campylobacter Speciesmentioning

confidence: 99%

OralCampylobacterspecies: Initiators of a subgroup of inflammatory bowel disease?

Zhang¹

2015

WJG

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In recent years, a number of studies detected a significantly higher prevalence of Campylobacter species such as Campylobacter concisus (C. concisus) in intestinal biopsies and fecal samples collected from patients with inflammatory bowel disease (IBD) compared to controls. Most of these Campylobacter species are not of zoonotic origin but are human oral Campylobacter species. Bacterial species usually cause diseases in the location where they colonize. However, C. concisus and other oral Campylobacter species are associated with IBD occurring at the lower parts of the gastrointestinal tract, suggesting that these Campylobacter species may have unique virulence factors that are expressed in the lower parts of the gastrointestinal tract.

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“…These are Gram-negative, chemoorganotrophic, non-sporeforming epsilonproteobacteria. Depending on the species, these can be slender, spirally curved-, curved- or straight- rods; with a single polar flagellum, bipolar or multiple flagella, or no flagellum; and microaerobic or anaerobic bacteria ( Vandamme et al, 2015 ). At the time of writing this paper, the genus Campylobacter comprised 32 species and 13 subspecies with validly published names 1 .…”

Section: Introductionmentioning

confidence: 99%

Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

Rivera-Mendoza¹,

Martínez-Flores²,

Santamaría³

et al. 2020

Front. Microbiol.

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The genus Campylobacter groups 32 Gram-negative bacteria species, several being zoonotic pathogens and a major cause of human gastroenteritis worldwide. Antibiotic resistant Campylobacter is considered by the World Health Organization as a high priority pathogen for research and development of new antibiotics. Genetic elements related to antibiotic resistance in the classical C. coli and C. jejuni species, which infect humans and livestock, have been analyzed in numerous studies, mainly focused on local geographical areas. However, the presence of these resistance determinants in other Campylobacter species, as well as in C. jejuni and C. coli strains distributed globally, remains poorly studied. In this work, we analyzed the occurrence and distribution of antibiotic resistance factors in 237 Campylobacter closed genomes available in NCBI, obtained from isolates collected worldwide, in different dates, from distinct hosts and comprising 22 Campylobacter species. Our data revealed 18 distinct genetic determinants, genes or point mutations in housekeeping genes, associated with resistance to antibiotics from aminoglycosides, β-lactams, fluoroquinolones, lincosamides, macrolides, phenicols or tetracyclines classes, which are differentially distributed among the Campylobacter species tested, on chromosomes or plasmids. Three resistance determinants, the bla OXA−493 and bla OXA−576 genes, putatively related to β-lactams resistance, as well as the lnu(AN2) gene, putatively related to lincosamides resistance, had not been reported in Campylobacter; thus, they represent novel determinants for antibiotic resistance in Campylobacter spp., which expands the insight on the Campylobacter resistome. Interestingly, we found that some of the genetic determinants associated with antibiotic resistance are Campylobacter species-specific; e.g., the bla OXA−493 gene and the T86V mutation in gyrA were found only in the C. lari group, whereas genes associated with aminoglycosides resistance were found only in C. jejuni and C. coli. Additional analyses revealed how are distributed the

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Campylobacter

Cited by 11 publications

References 153 publications

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

OralCampylobacterspecies: Initiators of a subgroup of inflammatory bowel disease?

Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

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