Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

Rivera-Mendoza, Daniel; Martínez-Flores, Irma; Santamaría, Rosa I.; Lozano, Luis; Bustamante, Vı́ctor H.; Pérez-Morales, Deyanira

doi:10.3389/fmicb.2020.513070

Cited by 20 publications

(28 citation statements)

References 88 publications

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“…Indeed, a high prevalence of bla OXA– 61 and tet(O) has been found on C. jejuni isolated from human, chicken, and cattle in Spain in a study where SNPs were not screened ( Mourkas et al, 2019 ) and on Campylobacter spp. in a global study analyzing 237 genomes from the RefSeq NCBI database ( Rivera-Mendoza et al, 2020 ). Moreover, AR determinants of aminoglycoside resistance on C. coli and quinolone resistance on C. jejuni were mainly found on genomes associated with multidrug resistance, together with AR determinants for β-lactams and tetracyclines.…”

Section: Discussionmentioning

confidence: 99%

“…for intrinsic production of β-lactamases in the absence of selective pressure ( Cody et al, 2012 ). The bla OXA– 61 gene, chromosomally encoded ( Rivera-Mendoza et al, 2020 ), can generate many variants with few SNPs, differing from other bla oxa -encoded proteins in only one (e.g., bla OXA– 193 ) or two amino acids (e.g., bla OXA– 489 ). The bla OXA– 61 gene and aminoglycoside resistance genes are confined to C. jejuni and C. coli genomes within Campylobacter species, while gyrA T86I SNP was mostly presented on C. jejuni ( Rivera-Mendoza et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…The bla OXA– 61 gene, chromosomally encoded ( Rivera-Mendoza et al, 2020 ), can generate many variants with few SNPs, differing from other bla oxa -encoded proteins in only one (e.g., bla OXA– 193 ) or two amino acids (e.g., bla OXA– 489 ). The bla OXA– 61 gene and aminoglycoside resistance genes are confined to C. jejuni and C. coli genomes within Campylobacter species, while gyrA T86I SNP was mostly presented on C. jejuni ( Rivera-Mendoza et al, 2020 ). Unfortunately, ACT to ATT mutation on gyrA position 86, the most common on C. coli strains with resistance to quinolones ( Zirnstein et al, 2000 ), was not included on PointFinder database, which includes ACA to ATA mutation, the most prevalent on C. jejuni .…”

Section: Discussionmentioning

confidence: 99%

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Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories

2021

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Campylobacter spp. are the most frequent agent of human gastroenteritis worldwide, and the spread of multidrug-resistant strains makes the clinical treatment difficult. The current study presents the resistome analysis of 39,798 Campylobacter jejuni and 11,920 Campylobacter coli genomes available in public repositories. Determinants of resistance to β-lactams (Be) and tetracyclines (Te) were the most frequent for both species, with resistance to quinolones (Qu) as the third most important on C. jejuni and to aminoglycosides (Am) on C. coli. Moreover, resistance to Te, Qu, and Am was frequently found in co-occurrence with resistance to other antibiotic families. Geographical differences on clonal complexes distribution were found for C. jejuni and on resistome genotypes for both C. jejuni and C. coli species. Attending to the resistome patterns by isolation source, three main clusters of genomes were found on C. jejuni genomes at antimicrobial resistance gene level. The first cluster was formed by genomes from human, food production animals (e.g., sheep, cow, and chicken), and food (e.g., dairy products) isolates. The higher incidence of tet(O), associated with tetracycline resistance, and the gyrA (T86I) single-nucleotide polymorphism (SNP), associated with quinolone resistance, among genomes from this cluster could be due to the intense use of these antibiotics in veterinary and human clinical settings. Similarly, a high incidence of tet(O) genes of C. coli genomes from pig, cow, and turkey was found. Moreover, the cluster based on resistome patterns formed by C. jejuni and C. coli genomes of human, turkey, and chicken origin is in agreement with previous observations reporting chicken or poultry-related environments as the main source of human campylobacteriosis infections. Most clonal complexes (CCs) associated with chicken host specialization (e.g., ST-354, ST-573, ST-464, and ST-446) were the CCs with the highest prevalence of determinants of resistance to Be, Qu, and Te. Finally, a clear trend toward an increase in the occurrence of Te and Qu resistance determinants on C. jejuni, linked to the spread of the co-occurrence of the blaOXA–61 and tet(O)-tet(O/W/O) genes and the gyrA (T86I) SNP, was found from 2001 to date in Europe.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories

2021

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“…Campylobacter bacteria are capable of intrinsic production of β-lactamases in the absence of selective (antibiotic) pressure. The bla OXA-61 gene has been shown to be widely distributed among Campylobacter isolated from poultry but has also been identified in isolates from non-food producing animals and environments [ 47 , 48 ]. On the other hand, β-lactam antibiotics are known to have limited efficacy against Campylobacter , and resistance to this class of antimicrobials appears to be mediated by both intrinsic resistance and β-lactamase production [ 26 ].…”

Section: Mechanisms Of Transfer Of Resistance Based On Examples Of Va...mentioning

confidence: 99%

Antibiotic Resistance in Bacteria—A Review

et al. 2022

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Background: A global problem of multi-drug resistance (MDR) among bacteria is the cause of hundreds of thousands of deaths every year. In response to the significant increase of MDR bacteria, legislative measures have widely been taken to limit or eliminate the use of antibiotics, including in the form of feed additives for livestock, but also in metaphylaxis and its treatment, which was the subject of EU Regulation in 2019/6. Numerous studies have documented that bacteria use both phenotypis and gentic strategies enabling a natural defence against antibiotics and the induction of mechanisms in increasing resistance to the used antibacterial chemicals. The mechanisms presented in this review developed by the bacteria have a significant impact on reducing the ability to combat bacterial infections in humans and animals. Moreover, the high prevalence of multi-resistant strains in the environment and the ease of transmission of drug-resistance genes between the different bacterial species including commensal flora and pathogenic like foodborne pathoges (E. coli, Campylobacter spp., Enterococcus spp., Salmonella spp., Listeria spp., Staphylococcus spp.) favor the rapid spread of multi-resistance among bacteria in humans and animals. Given the global threat posed by the widespread phenomenon of multi-drug resistance among bacteria which are dangerous for humans and animals, the subject of this study is the presentation of the mechanisms of resistance in most frequent bacteria called as “foodborne pathoges” isolated from human and animals. In order to present the significance of the global problem related to multi-drug resistance among selected pathogens, especially those danger to humans, the publication also presents statistical data on the percentage range of occurrence of drug resistance among selected bacteria in various regions of the world. In addition to the phenotypic characteristics of pathogen resistance, this review also presents detailed information on the detection of drug resistance genes for specific groups of antibiotics. It should be emphasized that the manuscript also presents the results of own research i.e., Campylobacter spp., E. coli or Enetrococcus spp. This subject and the presentation of data on the risks of drug resistance among bacteria will contribute to initiating research in implementing the prevention of drug resistance and the development of alternatives for antimicrobials methods of controlling bacteria.

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“…Erythromycin is still a first-choice drug in clinical management of the Campylobacteriosis (Rivera- Mendoza et al, 2020). Therefore, it is of vital importance to follow the resistance level of this antimicrobial in Campylobacter isolates.…”

Section: Antimicrobial Resistance Of C Jejunimentioning

confidence: 99%

Prevalence, Detection of Resistance Genes and Antimicrobial Resistance of Campylobacter jejuni in Broilers in North Macedonia

Angelovski¹,

Popova²,

Blagoevska³

et al. 2021

VSRR

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Campylobacter jejuni is one of the most important food borne pathogens. Since the start of the 21 st century C. jejuni is the leading cause for food borne enteritis. Another point of attention is the change in the antimicrobial resistance of this microorganism towards some critical antimicrobials used in the human and veterinary medicine. In this study samples were taken from three points in the broiler meat production (farm, slaughter line and cold storage of the meat before shipping to the market). A total of 283 samples (cloacal swabs, caeca and carcass swabs) were analyzed for the presence of C. jejuni. The isolates of C. jejuni were confirmed with the conventional microbiological method and with the use of multiplex PCR method. Both methods confirmed the overall prevalence of C. jejuni of 39.2%. In the second part of the study 108 confirmed isolates of C. jejuni were analyzed for the presence of resistance genes (CmeB, Bla oxa-61 , tet(O), aph-3-1 and aadE). The analysis in the third part of the study was concentrated on the antimicrobial resistance of the C. jejuni isolates towards three important antimicrobials (ciprofloxacin, erythromycin and tetracycline). The PCR method used revealed highest prevalence for Bla oxa-61 (25%), followed by CmeB and tet(O) genes (19.4%) and aadE with 13.9%. The aph-3-1 gene was not detected in none of the C. jejuni isolates. C. jejuni isolates in this study showed the highest resistance towards ciprofloxacin (63%) and tetracycline (50%) while the resistance towards erythromycin was very low (5.6%).

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Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

Cited by 20 publications

References 88 publications

Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories

Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories

Antibiotic Resistance in Bacteria—A Review

Prevalence, Detection of Resistance Genes and Antimicrobial Resistance of Campylobacter jejuni in Broilers in North Macedonia

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