<i>C</i>‐phycocyanin from the thermophilic blue‐green alga<i>Mastigocladus laminosus</i>, isolation, characterization and subunit composition

Binder, Andres; Wilson, Karl A.; Zuber, Herbert

doi:10.1016/0014-5793(72)80030-9

Cited by 57 publications

(9 citation statements)

References 25 publications

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“…Absorption spectra of a and /3 subunits in this experiment varied with the different methods and techniques used . Similar red shifts have been observed in a and /3 subunits from biliproteins of blue-green algae (Binder et al 1972 ;Torjensen & Sletten 1972) and Rhodomonas lens (MacCoil & Berns 1979), and various values of X max have been reported by other investigators (Fujimori & Pecci 1971 ;Brooks & Gantt 1973 ;Gantt & Lipshultz 1974 ;Glazer 1981) . The spectroscopic behavior of the a and /3 subunits obviously represents a very complicated phenomenon .…”

Section: Resultssupporting

confidence: 81%

Spectroscopic behavior of biliprotein from Porphyra tenera upon denaturation with urea and sodium dodecyl sulfate (SDS)

1984

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Section: Resultssupporting

confidence: 81%

Spectroscopic behavior of biliprotein from Porphyra tenera upon denaturation with urea and sodium dodecyl sulfate (SDS)

1984

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“…The amino acid compositions were based on mol.wt. 30000, the weight of the 3S monomer for other Cphycocyanins determined by sedimentation equilibrium at pH3.9 (Kao et al, 1971), and agreed with the sum of the molecular weights of the two chains found by sodium dodecyl sulphate-polyacrylamide-gel electrophoresis in some but not all cases (Glazer & Cohen-Bazire, 1971; Bennett & Bogorad, 1971;O'Carra & Killilea, 1971;Binder et al, 1972;Torjesen & Sletten, 1972). Partial specific volumes were calculated (Cohn & Edsall, 1943) from these data to be 0.733 and 0.734ml/g for species Sy I and Sy III respectively.…”

Section: Resultssupporting

confidence: 64%

Comparison of the biliproteins from two strains of the thermophilic cyanophyte Synechococcus lividus

MacColl

Edwards

Mulks

et al. 1974

Biochemical Journal

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C-Phycocyanins from two thermophilic strains of Synechococcus lividus that grow within different temperature ranges have been shown to be unalike. The aggregation ability of these two C-phycocyanins in sedimentation-velocity experiments varied dramatically. Surprisingly, the aggregation properties of mesophilic C-phycocyanins were found to lie between those of the two thermophilic proteins. Under identical conditions at pH7.0, one thermophilic protein (Sy I) was composed of 17S and larger aggregates, whereas the other (Sy III) was an almost homogeneous 6S aggregate. Mesophilic C-phycocyanins have a mixture of 6S, 11S and less stable 17S aggregates under these conditions. Amino acid analysis, absorption spectra, immunochemistry and fluorescence polarization all indicated differences in the composition and properties of the thermophilic proteins, which suggest that they have different modes of adaptation to very high temperatures. Allophycocyanins from the two strains of S. lividus were also purified and studied, but unlike the C-phycocyanins no major differences were found between them. Allophycocyanin was homogeneous at pH6.0, with a sedimentation coefficient of 5.54S and mol.wt. 1.03x10(5), as determined by sedimentation-equilibrium measurements.

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“…The dialyzed phycocyanin was then placed in a 2.5 × 30 cm hydroxylapatite column and the two main fractions were pooled following a stepwise elution with phosphate buffers of increasing ionic strength at pH 7.0, as follows: The first fraction was eluted between 2.5 mM and 70 mM and represented c-phycocyanin. The fraction containing c-phycocyanin was purified by chromatography on a 1.5 × 20 cm DEAE Sephadex A-50, according to Binder et al [16,17]. …”

Section: Methodsmentioning

confidence: 99%

Stable Isolation of Phycocyanin from Spirulina platensis Associated with High-Pressure Extraction Process

Seo

Choi

Park³

et al. 2013

IJMS

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A method for stably purifying a functional dye, phycocyanin from Spirulina platensis was developed by a hexane extraction process combined with high pressure. This was necessary because this dye is known to be very unstable during normal extraction processes. The purification yield of this method was estimated as 10.2%, whose value is 3%–5% higher than is the case from another conventional separation method using phosphate buffer. The isolated phycocyanin from this process also showed the highest purity of 0.909 based on absorbance of 2.104 at 280 nm and 1.912 at 620 nm. Two subunits of phycocyanin namely α-phycocyanin (18.4 kDa) and β-phycocyanin (21.3 kDa) were found to remain from the original mixtures after being extracted, based on SDS-PAGE analysis, clearly demonstrating that this process can stably extract phycocyanin and is not affected by extraction solvent, temperature, etc. The stability of the extracted phycocyanin was also confirmed by comparing its DPPH (α,α-diphenyl-β-picrylhydrazyl) scavenging activity, showing 83% removal of oxygen free radicals. This activity was about 15% higher than that of commercially available standard phycocyanin, which implies that the combined extraction method can yield relatively intact chromoprotein through absence of degradation. The results were achieved because the low temperature and high pressure extraction effectively disrupted the cell membrane of Spirulina platensis and degraded less the polypeptide subunits of phycocyanin (which is a temperature/pH-sensitive chromoprotein) as well as increasing the extraction yield.

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C‐phycocyanin from the thermophilic blue‐green algaMastigocladus laminosus, isolation, characterization and subunit composition

Cited by 57 publications

References 25 publications

Spectroscopic behavior of biliprotein from Porphyra tenera upon denaturation with urea and sodium dodecyl sulfate (SDS)

Spectroscopic behavior of biliprotein from Porphyra tenera upon denaturation with urea and sodium dodecyl sulfate (SDS)

Comparison of the biliproteins from two strains of the thermophilic cyanophyte Synechococcus lividus

Stable Isolation of Phycocyanin from Spirulina platensis Associated with High-Pressure Extraction Process

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