Botrytis euroamericana, a new species from peony and grape in North America and Europe

Abstract

A novel species of Botrytis isolated from peony in Alaska, USA, and grape in Trento District, Italy, was identified based on morphology, pathogenicity, and sequence data. The grape and peony isolates share sequence homology in the glyceraldehyde-3-phosphate dehydrogenase (G3PDH), heat shock protein 60 (HSP60), DNA-dependent RNA polymerase subunit II (RPB2), and necrosis- and ethylene-inducing protein 1 and 2 (NEP1 and NEP2) genes that place them in a distinct group closely related to B. aclada, a globally distributed pathogen of onions. Genetic results were corroborated with morphological and pathogenicity trials that included two isolates of B. cinerea and two isolates of B. paeoniae from peony in Alaska and one isolate of B. aclada. The authors observed differences in colony and conidia morphology and ability to cause lesions on different host tissues that suggest that the grape and peony isolates represent a distinct species. Most notably, the grape and peony isolates did not colonize onion bulbs, whereas B. aclada readily produced lesions and prolific sporulation on onion tissue. The new species Botrytis euroamericana is described herein.

Identification and characterization of Botrytis medusae, a novel cryptic species causing grey mould on wine grapes in Australia

“…Several studies have shown that B. cinerea exists within a morphologically cryptic species complex (Giraud et al ., , ; Albertini et al ., ; Fournier et al ., , ; Walker et al ., ; Lorenzini & Zapparoli, ; Liu et al ., ; Garfinkel et al ., ). Distinguishing different Botrytis species in sympatry and defining their contributions to disease could assist in adapting control strategies (Walker, ).…”

“…Clade I includes the isolates from group I and II species B. californica , B. calthae , B. cinerea , B. eucalypti , B. fabae , B. pelargonii , B. pseudocinerea and B. sinoviticola . Recently, a new clade II Botrytis species, B. euroamericana , was found on grapevine in Italy (Lorenzini & Zapparoli, ; Garfinkel et al ., ).…”

“…Botrytis species have been previously divided into two clades, clade I and clade II. Clade I species exclusively infect eudicots, while clade II species infect monocots and eudicots (Staats et al, 2005) (Lorenzini & Zapparoli, 2014;Garfinkel et al, 2017).…”

Comparative genomics of plant pathogenic Botrytis species with distinct host specificity

“…The fungal genus Botrytis comprises ~ 35 species, which all interact with plants [1, 2]. With one exception, they are notorious pathogens with a necrotrophic infection behaviour, i.e.…”

Botrytis genus fungi as causal agents of legume diseases: A review

“…Pathogenicity tests showed the ability of B. aclada, B. euroamericana, and B. paeoniae to form lesions on faba bean leaves (Garfinkel et al, 2017). B. aclada is a specialized pathogen of onion (Allium cepa) but also could cause primary lesions on the non-host plant that were significantly smaller than B. cinerea, B. euroamericana, and B. paeoniae caused lesions.…”