Botrytis genus fungi as causal agents of legume diseases: A review

Brauna-Morževska, Elīna; Bankina, B.; Kaņeps, Jānis

doi:10.22616/rrd.25.2019.050

Cited by 7 publications

(3 citation statements)

References 42 publications

(46 reference statements)

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“…To our knowledge, this is the first report of the presence of B. caroliniana in Europe. Other species, such as B. fabiopsis or the new species B. euroamericana, have been observed in Europe; however, information on their geographical presence is scarce [36,37]. B. caroliniana EXF-17025 (No.…”

Section: Identification Of the Isolated Fungal Strains From Vineyards...mentioning

confidence: 99%

A Pipeline to Investigate Fungal–Fungal Interactions: Trichoderma Isolates against Plant-Associated Fungi

Dourou

Porta

2023

JoF

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Soil fungi play essential roles in ecosystems, forming complex interaction networks with bacteria, yeasts, other fungi, or plants. In the framework of biocontrol strategies, Trichoderma-based fungicides are at the forefront of research as an alternative to synthetic ones. However, the impact of introducing new microbial strain(s) on the soil microbiome of a habitat is not well-explored. Aiming to identify a quantitative method to explore the complex fungal interactions, we isolated twelve fungi from three Italian vineyards and identified three strains of the Trichoderma genus in addition to nine more plant-associated fungi of different genera. Investigating in dual nucleation assay fungal–fungal interactions, we recognised two types of interaction: neutral or antagonistic. All three Trichoderma strains displayed a slight inhibitory behaviour against themselves. Trichoderma strains showed a mutually intermingling growth with Aspergillus aculeatus and Rhizopus arrhizus but antagonistic behaviour against the plant pathogens Alternaria sp., Fusarium ramigenum, and Botrytis caroliniana. Yet, in some cases, antagonistic behaviour by Trichoderma fungi was also observed against plant-promoting fungi (e.g., Aspergillus piperis and Penicillium oxalicum). Our study highlights the importance of studying the interactions between fungi, aiming to clarify better the impact of fungal-based biological fungicides in the soil communities, and offers a pipeline for further applications.

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Section: Identification Of the Isolated Fungal Strains From Vineyards...mentioning

confidence: 99%

A Pipeline to Investigate Fungal–Fungal Interactions: Trichoderma Isolates against Plant-Associated Fungi

Dourou

Porta

2023

JoF

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“…These findings were supported by subsequent research (Fan et al, 2015;Elad et al, 2016). B. fabiopsis has been found in Latvia on faba bean (Brauna-Morževska et al, 2019). DNA analysis showed that B. cinerea comprised two distinct populations leading to the separation of B. pseudocinerea, which is similar in morphology but genetically distinct from B. cinerea (Plesken et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…DNA analysis showed that B. cinerea comprised two distinct populations leading to the separation of B. pseudocinerea, which is similar in morphology but genetically distinct from B. cinerea (Plesken et al, 2015). Thus, at least four species of Botrytis have been associated with the chocolate spot disease of faba bean leading to hypothesize that the causal agents of a chocolate spot on faba bean in northern Europe might be more complex than the traditional view (Brauna-Morževska et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Botrytis four species are associated with chocolate spot disease of faba bean in Latvia

Bankina

Stoddard

Kaņeps

et al. 2021

Zemdirbyste-Agriculture

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Faba bean (Vicia faba L.) is gaining importance as a crop in northern Europe. In this region, the most important disease of faba bean is chocolate spot disease, attributed to the pathogen Botrytis fabae. However, other Botrytis species have been found to contribute to the disease. Hence, it was decided to isolate fungi from faba bean plants showing symptoms of chocolate spot disease in Latvia, identify the Botrytis species using the DNA sequences of three definitive genes, evaluate the morphological diversity of the isolates in vitro and, finally, to determine the pathogenicity of the isolates in a detached-leaf test. In addition to B. fabae, B. cinerea, B. pseudocinerea and B. fabiopsis were all identified. Phylogenetic analysis of the DNA sequences put all the obtained 44 isolates unequivocally into clusters with known examples of each species. Every species showed wide diversity in its in vitro colour, texture and growing pattern of mycelium, production of sclerotia and pigmentation of the growing medium with much overlap between species showing that this method is not adequate for species discrimination. B. fabae produced the largest lesions on infected leaves, followed closely by B. pseudocinerea and B. cinerea, while B. fabiopsis produced much smaller lesions. The results show that chocolate spot disease of faba bean is attributable to Botrytis four species in northern Europe. This knowledge needs to be considered when controlling the disease by genetic or agronomic means.

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