<i>Borrelia lusitaniae</i>and Green Lizards (<i>Lacerta viridis</i>), Karst Region, Slovakia

Majláthová, Viktória; Majláth, Igor; Derdáková, Markéta; Víchová, Bronislava; Peťko, Branislav

doi:10.3201/eid1212.060784

Cited by 59 publications

(55 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Western Slovakia, high prevalence of B. garinii and B. afzelii in I. ricinus was reported (Hanincová et al 2000), but also B. valaisiana and B. burgdorferi s.s. were found there. B. lusitaniae as a dominant species was detected locally in the Slovak karst (Majláthová et al 2006). In Bohemia, B. afzelii was the predominant species (Derdáková et al 2003).…”

Section: Discussionmentioning

confidence: 95%

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

et al. 2008

Self Cite

View full text Add to dashboard Cite

The paper analyses the results of serological examinations of domestic, farm and free-living animals from different regions of Slovakia, Southern Moravia, Southern Bohemia and Southern Poland using ELISA, indirect hemagglutination assay (IHA) and Western blot (WB). In Slovakia, significantly higher seroprevalence was recorded in dogs (33.5%) than in horses (26.5%), cattle (22.5%), sheep (16.6%) and rodents (17.8%) by using a mixture of Borrelia garinii, B. afzelii, B. burgdorferi sensu stricto (s.s.) antigens in ELISA. Seroprevalence in horses was significantly higher than in sheep and rodents, and seroprevalence in cattle was significantly higher than in rodents. By using IHA in free-living species, the highest seropositivity rates were detected in fallow deer (40.7%) compared with moufflons (16.6%), pheasants (8.0%) and pigeons (1.2%). When testing sera of horses, dogs and cattle from Slovakia by using different Slovak B. burgdorferi sensu lato (s.l.) isolates as antigens in ELISA, significantly higher seroprevalence of anti-Borrelia IgG antibodies and consistency of positive and negative findings was detected in comparison when American isolates were used. In WB analyses using the Eurocarpathian antigens, dog sera from Eastern Slovakia and Southern Moravia showed statistically insignificant differences in sensitivity and consistency of positive and negative findings. By using different methods and antigens in the same group of dog sera, significant differences in seroprevalence were only found in IHA with a mixture of Euroamerican B.b.s.l and WB CB26 B.b.s.s. In addition to other factors, the complexity of the standardization of the assay system with regard to the genetic and geographical heterogeneity of B. burgdorferi s.l. isolates used as antigens is also discussed.

show abstract

Section: Discussionmentioning

confidence: 95%

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent studies have reported on findings of Borrelia burgdorferi s.l. in lacertid lizards and their ticks Ixodes ricinus collected in Slovakia and Hungary (Földvári et al 2009;Majláthová et al 2006). In this light, the importance of tortoises and their ticks in maintaining natural foci of Q-fever should not be undervalued.…”

Section: Discussionmentioning

confidence: 96%

Tortoise tick Hyalomma aegyptium as long term carrier of Q fever agent Coxiella burnetii—evidence from experimental infection

et al. 2010

View full text Add to dashboard Cite

The experimental study investigated the ability of tortoise tick Hyalomma aegyptium to play a role in forming and maintaining natural foci of Q fever. We tested the competence of H. aegyptium larvae to acquire Coxiella burnetii infection from mammals, serve as a C. burnetii vector between mammalian hosts, and be a long-term carrier of C. burnetii, including interstadial transmission. H. aegyptium larvae were allowed to feed on guinea pigs experimentally infected with C. burnetii. Engorged larvae molted to nymphs, some of which were preserved in 96% ethanol and later examined by polymerase chain reaction (PCR) using C. burnetii-specific primers (CBCOS, CBCOE). Prevalence of C. burnetii among these nymphs was 5.6% (n=235). Remaining nymphs then fed on other, C. burnetii-negative guinea pigs; and according to results of both, micro-agglutination reaction, and ELISA, they successfully transmitted C. burnetii to those new hosts. Detached engorged nymphs molted to adults, which were kept alive long term and then placed in 96% ethanol 383 days post-infection. Thereafter, they were examined by PCR in the same manner as were the nymphs. Prevalence of C. burnetii among adult H. aegyptium was 28.9% (n=90). According to our results, tortoise-specific ticks have indisputable potential in the epidemiology of Q fever natural foci.

show abstract

“…The PCR products of the 18S rDNA were further analysed by restriction fragment length polymorphism (RFLP), according to previously described protocol (Majláthová et al 2006). For each positive sample, 13 µl amplified DNA was digested at 65°C overnight in a solution containing 5U of Tru1I (300 U/ml) and 1× buffer R (Fermentas).…”

Section: Rflp Analysismentioning

confidence: 99%

Apicomplexan parasites of red foxes (Vulpes vulpes) in northeastern Poland

Karbowiak

Majláthová

Hapunik

et al. 2010

Acta Parasitologica

View full text Add to dashboard Cite

Molecular detection of apicomplexan parasites in splenic samples of red foxes collected from northeastern Poland was conducted by PCR amplification of a fragment of the 18S rRNA spanning the V4 gene region of Apicomplexa. Positive PCR products were further analysed by restriction fragment length polymorphism (RFLP) and sequencing to identify species. One hundred and eleven red foxes (Vulpes vulpes) were acquired from 15 localities in the Mazovian Province and 27 foxes were acquired from the Mazurian Lakeland. Apicomplexan 18S rDNA was detected in 15.9% of 138 fox spleens examined. Three apicomplexan species were identified: Hepatozoon canis was detected in 11.6% of the spleen samples, Toxoplasma gondii was detected in 3.6% of the spleen samples and a Babesia sp. was sequenced from 1 sample (0.7%). This data represent the first record of H. canis, T. gondii and a B. sp. from naturally infected red foxes in Poland. Infected foxes may act as sylvatic reservoirs of these apicomplexan parasites as well as serving as a source of infection for arthropod definitive hosts and vectors.

show abstract

Borrelia lusitaniaeand Green Lizards (Lacerta viridis), Karst Region, Slovakia

Abstract: TOC summary line: The green lizard is implicated in the transmission cycle of B. lusitaniae.

Cited by 59 publications

References 35 publications

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

Tortoise tick Hyalomma aegyptium as long term carrier of Q fever agent Coxiella burnetii—evidence from experimental infection

Apicomplexan parasites of red foxes (Vulpes vulpes) in northeastern Poland

Contact Info

Product

Resources

About