Boehmeria niveaStimulates Glucose Uptake by Activating Peroxisome Proliferator-Activated Receptor Gamma in C2C12 Cells and Improves Glucose Intolerance in Mice Fed a High-Fat Diet

Abstract

We examined the antidiabetic property of Boehmeria nivea (L.) Gaud. Ethanolic extract of Boehmeria nivea (L.) Gaud. (EBN) increased the uptake of 2-[N-(nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose in C2C12 myotubes. To examine the mechanisms underlying EBN-mediated increase in glucose uptake, we examined the transcriptional activity and expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), a pivotal target for glucose metabolism in C2C12 myotubes. We found that the EBN increased both the transcriptional activity and mRNA expression levels of PPAR-γ. In addition, we measured phosphorylation and expression levels of other targets of glucose metabolism, such as AMP-activated protein kinase (AMPK) and protein kinase B (Akt/PKB). We found that EBN did not alter the phosphorylation or expression levels of these proteins in a time- or dose-dependent manner, which suggested that EBN stimulates glucose uptake through a PPAR-γ-dependent mechanism. Further, we investigated the antidiabetic property of EBN using mice fed a high-fat diet (HFD). Administration of 0.5% EBN reduced the HFD-induced increase in body weight, total cholesterol level, and fatty liver and improved the impaired fasting glucose level, blood insulin content, and glucose intolerance. These results suggest that EBN had an antidiabetic effect in cell culture and animal systems and may be useful for preventing diabetes.

Laxative and antioxidant effects of ramie (Boehmeria nivea L.) leaf extract in experimental constipated rats

“…It is thought that RLE rich in dietary fiber may shorten intestinal transit time, leading to the decreased number of fecal pellets in the colon. In addition, these results were similar to those of previous reports (Attaluri et al, 2011;Han et al, 2017;Kakino et al, 2010;Kim, Lee, et al, 2013;Kim, Sung, et al, 2013;Kim et al, 2018;Méité et al, 2010;Sathyanathan et al, 2013;Seo et al, 2013). Méité et al (2010) reported that Mareya micrantha (Benth.)…”

“…These findings were also similar to Wintola et al (2010). Food intake and water consumption are considered important factors for the assessment of constipation symptoms and therapeutic effects (Kim, Lee, et al, 2013;Kim, Sung, et al, 2013). In our study, food intake and water consumption increased in response to RLE treatment after constipation induction.…”

“…Effects of ramie leaf extract on body weight gain, food intake, and water consumption in rats Various medicinal foods and plants containing many bioactive compounds have recently emerged as novel therapeutic materials for the treatment or prevention of constipation (Aslam & Janbaz, 2019;Attaluri et al, 2011;Kakino et al, 2010;Kim, Lee, et al, 2013;Kim, Sung, et al, 2013;Langmead & Rampton, 2001;Méité et al, 2010;Sathyanathan et al, 2013;Seo et al, 2013).…”

Extract of Boehmeria nivea Suppresses Mast Cell-Mediated Allergic Inflammation by Inhibiting Mitogen-Activated Protein Kinase and Nuclear Factor-κB

“…Consistent with previous studies, we found that Boehmeria nivea leaves contained abundant polyphenol components. Recent studies have shown that BNE exerts anti-inflammatory [ 6 ], antioxidant [ 15 ], anti-hepatitis B virus [ 5 ], anti-colitis [ 7 ], anti-obesity [ 20 ], and anti-diabetic effects [ 21 ]. Based on these known effects, we assumed that BNE might play a role in suppressing IgE-mediated mast cell inflammation and could be used for treating allergic diseases in the future.…”

Effects of Lonicera japonica Thunb. on Type 2 Diabetes via PPAR-γ Activation in Rats

“…In the type 2 diabetes, there are crucial molecules that could be therapeutic targets for the treatment of type 2 diabetes. Peroxisome proliferator‐activated receptor gamma (PPAR‐ γ ) is responsible for glucose uptake and regulation of fatty acid, and PPAR‐ γ agonists have been wildly used for the treatment of diabetes (Kim et al , ). In addition, insulin receptor subunit‐1 (IRS‐1) is a trigger molecule to transmit the insulin signaling to downstream molecules (Draznin, ).…”