Extract of Boehmeria nivea Suppresses Mast Cell-Mediated Allergic Inflammation by Inhibiting Mitogen-Activated Protein Kinase and Nuclear Factor-κB

Lim, Ji Ye; Lee, Ji‐Hyun; Lee, Bori; Kim, Mi Ae; Lee, Young-Mi; Kim, Dae‐Ki; Choi, Jin Kyeong

doi:10.3390/molecules25184178

Cited by 12 publications

(12 citation statements)

References 37 publications

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“…ROS signaling is necessary for mast cell activation (38). NF-kB/MAPK signaling can mediate allergic inflammation promoted by mast cell activation (39). The effect of ROS/MAPK/NF-kB signaling on allergic inflammation merits further study.…”

Section: Discussionmentioning

confidence: 99%

MiR-154-5p-MCP1 Axis Regulates Allergic Inflammation by Mediating Cellular Interactions

Kim

Kwon

et al. 2021

Front. Immunol.

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In a previous study, we have demonstrated that p62, a selective receptor of autophagy, can regulate allergic inflammation. In the present study, microRNA array analysis showed that miR-154-5p was increased by antigen (DNP-HSA) in a p62-dependent manner in rat basophilic leukemia cells (RBL2H3). NF-kB directly increased the expression of miR-154-5p. miR-154-5p mediated in vivo allergic reactions, including passive cutaneous anaphylaxis and passive systemic anaphylaxis. Cytokine array analysis showed that antigen stimulation increased the expression of MCP1 in RBL2H3 cells in an miR-154-5p-dependent manner. Reactive oxygen species (ROS)-ERK-NF-kB signaling increased the expression of MCP1 in antigen-stimulated RBL2H3 cells. Recombinant MCP1 protein induced molecular features of allergic reactions both in vitro and in vivo. Anaphylaxis-promoted tumorigenic potential has been known to be accompanied by cellular interactions involving mast cells, and macrophages, and cancer cells. Our experiments employing culture medium, co-cultures, and recombinant MCP1 protein showed that miR-154 and MCP1 mediated these cellular interactions. MiR-154-5p and MCP1 were found to be present in exosomes of RBL2H3 cells. Exosomes from PSA-activated BALB/C mouse induced molecular features of passive cutaneous anaphylaxis in an miR-154-5p-dependent manner. Exosomes from antigen-stimulated RBL2H3 cells enhanced both tumorigenic and metastatic potentials of B16F1 melanoma cells in an miR-154-5p-dependent manner. Exosomes regulated both ROS level and ROS mediated cellular interactions during allergic inflammation. Our results indicate that the miR-154-5p-MCP1 axis might serve as a valuable target for the development of anti-allergy therapeutics.

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Section: Discussionmentioning

confidence: 99%

MiR-154-5p-MCP1 Axis Regulates Allergic Inflammation by Mediating Cellular Interactions

Kim

Kwon

et al. 2021

Front. Immunol.

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“…Moreover, quercetin is known to ameliorate the pathogenic process of asthma by decreasing IL-4 and IFN-γ synthesis and by regulating Th1/Th2 balance [137]. Recent evidence showed that the administration of polyphenol-rich ethanolic extract of Boehmeria nivea (caffeic acid, catechin, epicatechin, β-sissterol, rutin, luteolin-7glucoside, naringin, hesperidin, chlorogenic acid, and tangeretin) reduces allergic response in mice by suppressing mast cell mediated inflammation, decreasing TNF-α, IL-1β, IL-6, Th2, extracellular-signal-regulated kinase (ERK) and Mitogen-Activated Protein Kinase (MAPK) expression [138]. In murine asthma models, it was found that sesamin (rich in flavonoids) reduced allergic inflammation induce by asthma and airway hyperresponsiveness (AHR), making them an effective adjunct for the treatment of asthma [139].…”

Section: Polyphenols In Allergic Asthma and Rhinitismentioning

confidence: 99%

Immunomodulatory Effects of Dietary Polyphenols

et al. 2021

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Functional and nutraceutical foods provide an alternative way to improve immune function to aid in the management of various diseases. Traditionally, many medicinal products have been derived from natural compounds with healing properties. With the development of research into nutraceuticals, it is becoming apparent that many of the beneficial properties of these compounds are at least partly due to the presence of polyphenols. There is evidence that dietary polyphenols can influence dendritic cells, have an immunomodulatory effect on macrophages, increase proliferation of B cells, T cells and suppress Type 1 T helper (Th1), Th2, Th17 and Th9 cells. Polyphenols reduce inflammation by suppressing the pro-inflammatory cytokines in inflammatory bowel disease by inducing Treg cells in the intestine, inhibition of tumor necrosis factor-alpha (TNF-α) and induction of apoptosis, decreasing DNA damage. Polyphenols have a potential role in prevention/treatment of auto-immune diseases like type 1 diabetes, rheumatoid arthritis and multiple sclerosis by regulating signaling pathways, suppressing inflammation and limiting demyelination. In addition, polyphenols cause immunomodulatory effects against allergic reaction and autoimmune disease by inhibition of autoimmune T cell proliferation and downregulation of pro-inflammatory cytokines (interleukin-6 (IL-6), IL-1, interferon-γ (IFN-γ)). Herein, we summarize the immunomodulatory effects of polyphenols and the underlying mechanisms involved in the stimulation of immune responses.

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“…Antihistamines and steroids are effective drugs in the treatment of allergic diseases, but serious side effects hinder their long-term use ( Lim et al, 2020 ). TMDCD is a Chinese herbal compound optimized based on the compound of MXGSD and GuoMinKang, and also is a summary of clinical experience on allergic asthma by professor Wang Qi from Beijing university of Chinese medicine.…”

Section: Introductionmentioning

confidence: 99%

Tuo-Min-Ding-Chuan Decoction Alleviate Ovalbumin-Induced Allergic Asthma by Inhibiting Mast Cell Degranulation and Down-Regulating the Differential Expression Proteins

Qin

Jiang

et al. 2021

Front. Pharmacol.

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Allergic asthma is a stubborn chronic inflammatory disease, and is considered a co-result of various immune cells, especially mast cells, eosinophils and T lymphocytes. At present, the treatment methods of allergic asthma are limited and the side effects are obvious. Traditional Chinese medicine has been used to treat diseases for thousands of years in China. One such example is the treatment of allergic asthma, which take the characteristics of less adverse reactions and obvious curative effect. Tuo-Min-Ding-Chuan Decoction (TMDCD) is a traditional Chinese medicine compound for the treatment of allergic asthma optimized from Ma-Xing-Gan-Shi Decoction (MXGSD), which was put forward in Treatise on Febrile Diseases by Zhang Zhongjing in the Eastern Han Dynasty. The compound shows a significant clinical effect, but the mechanism of its influence on the immune system is still unclear. The purpose of this study was to observe whether TMDCD could alleviate the symptoms of ovalbumin (OVA) challenged allergic asthma mice, and to explore its immune regulatory mechanism, especially on mast cell (MC) degranulation. The results showed TMDCD could not only reduce the airway hyperresponsiveness (AHR), inflammatory cell infiltration and mucus secretion in the lung tissue of OVA challenged mice, but also decrease the levels of total IgE, OVA-specific IgE, histamine and LTC4 in serum. We found that TMDCD can downregulate the expression of Fractalkine, Tryptase ε, IL-25, CCL19, MCP-1, OX40L, Axl, CCL22, CD30, G-CSF, E-selectin, OPN, CCL5, P-selectin, Gas6, TSLP in OVA challenged mice serum by using mouse cytokines antibody array. It has been reported in some literatures that these differentially expressed proteins are related to the occurrence of allergic asthma, such as tryptase ε, MCP-1, CCL5, etc. can be released by MC. And the results of in vitro experiments showed that TMDCD inhibited the degranulation of RBL-2H3 cells stimulated by DNP-IgE/BSA. Taken together, we made the conclusion that TMDCD could reduce the infiltration of inflammatory cells in lung tissue and alleviate airway remodeling in mice with allergic asthma, showed the effects of anti-inflammatory and antiasthmatic. TMDCD could also reduce the levels of IgE, histamine, LTC4, Tryptase ε, and other MC related proteins in the serum of allergic asthma mice, and the in vitro experiments showed that TMDCD could inhibit IgE mediated degranulation and histamine release of RBL-2H3 cells, proved its anti allergic effect.

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Extract of Boehmeria nivea Suppresses Mast Cell-Mediated Allergic Inflammation by Inhibiting Mitogen-Activated Protein Kinase and Nuclear Factor-κB

Cited by 12 publications

References 37 publications

MiR-154-5p-MCP1 Axis Regulates Allergic Inflammation by Mediating Cellular Interactions

MiR-154-5p-MCP1 Axis Regulates Allergic Inflammation by Mediating Cellular Interactions

Immunomodulatory Effects of Dietary Polyphenols

Tuo-Min-Ding-Chuan Decoction Alleviate Ovalbumin-Induced Allergic Asthma by Inhibiting Mast Cell Degranulation and Down-Regulating the Differential Expression Proteins

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